Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Lactic acid bacterial inoculant effects on the vitamin content of alfalfa and Chinese leymus silage

  • Jia, Tingting (Department of grassland production and utilization, College of Grassland Science and Technology, China Agricultural University) ;
  • Sun, Zhiqiang (Department of grassland production and utilization, College of Grassland Science and Technology, China Agricultural University) ;
  • Gao, Run (Department of grassland production and utilization, College of Grassland Science and Technology, China Agricultural University) ;
  • Yu, Zhu (Department of grassland production and utilization, College of Grassland Science and Technology, China Agricultural University)
  • Received : 2019.02.15
  • Accepted : 2019.04.20
  • Published : 2019.12.01
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Abstract

Objective: Information regarding the vitamin content of silage is limited. This study investigated the changes in the vitamin content of alfalfa and Chinese leymus silages with or without a lactic acid bacterial inoculant. Methods: Alfalfa at the early flowering stage and Chinese leymus at the full-bloom stage were harvested. The treatments for each forage type were control (deionized water only) and $1{\times}10^6$ colony-forming units Lactobacillus plantarum (LP)/g fresh matter. After 45 days of ensiling, all silages were sampled for evaluating the vitamin content, fermentation quality and chemical composition. Results: The LP inoculant decreased the pH value and ammonia nitrogen content of the alfalfa and Chinese leymus silages and significantly (p<0.05) increased the lactic acid, acetic acid concentrations and Flieg's points. Prior to ensiling, the levels of five B-group vitamins (thiamin, riboflavin, niacin, pantothenic acid, and pyridoxine) and ${\alpha}$-tocopherol in alfalfa were significantly (p<0.01) higher than those in Chinese leymus. Ensiling decreased the levels of the five B-group vitamins in both alfalfa and Chinese leymus while increasing the ${\alpha}$-tocopherol content of Chinese leymus. The thiamin, riboflavin, niacin and pantothenic acid levels in the LP-treated silage were significantly (p<0.05) lower than those in the untreated silage for the alfalfa and Chinese leymus. The ${\alpha}$-tocopherol content in the LP-treated alfalfa silage was significantly (p<0.05) higher than that in the untreated alfalfa silage. There was no significant (p>0.05) difference in pyridoxine content between the untreated and LP-treated silages for both forages. Conclusion: With or without LP inoculation, the levels of the five B-group vitamins (thiamin, riboflavin, niacin, pantothenic acid, and pyridoxine) in alfalfa and Chinese leymus decreased after 45 days of ensiling, while the ${\alpha}$-tocopherol content of Chinese leymus increased. The LP inoculant improved the fermentation quality of both the alfalfa and Chinese leymus silages but increased the thiamin, riboflavin, niacin, and pantothenic acid loss in the two forages after fermentation.

Keywords

References

  1. Jensen SK, Johannsen AKB, Hermansen JE. Quantitative secretion and maximal secretion capacity of retinol, ${\beta}$-carotene and ${\alpha}$-tocopherol into cows' milk. J Dairy Res 1999;66:511-22. https://doi.org/10.1017/S0022029999003805
  2. Keuth S, Bisping B. Formation of vitamins by pure cultures of tempe moulds and bacteria during the tempe solid substrate fermentation. J Appl Bacteriol 1993;75:427-34. https://doi.org/10.1111/j.1365-2672.1993.tb02798.x
  3. Elgersma A, Soegaard K, Jensen SK. Vitamin contents in forage herbs. Aspects Appl Biol 2012;115:75-80.
  4. McDowell LR. Vitamins in animal nutrition: comparative aspects to human nutrition. San Diego, CA, USA: Academic Press; 1989.
  5. Booth VH. The ${\alpha}$-tocopherol content of forage crops. J Sci Food Agric 1964;15:342-4. https://doi.org/10.1002/jsfa.2740150512
  6. Hidiroglou M, Batra TR, Roy GL. Changes in plasma ${\alpha}$-tocopherol and selenium of gestating cows fed hay or silage. J Dairy Sci 1994;77:190-5. https://doi.org/10.3168/jds.S0022-0302(94)76941-1
  7. Ballet N, Robert JC, Williams PEV. Vitamins in forages. In: Givens DI, Owen E, Axford RFE, Omed HM, editors. Forage evaluation in ruminant nutrition. Oxon, UK: CABI Publishing; 2000. p. 399-431 (Chapter 19)
  8. Shingfield KJ, Salo-Vaananen P, Pahkala E, et al. Effect of forage conservation method, concentrate level and propylene glycol on the fatty acid composition and vitamin content of cows' milk. J Dairy Res 2005;72:349-61. https://doi.org/10.1017/S0022029905000919
  9. Liu QH, Shao T, Bai YF. The effect of fibrolytic enzyme, Lactobacillus plantarum and two food antioxidants on the fermentation quality, alpha-tocopherol and beta-carotene of high moisture napier grass silage ensiled at different temperatures. Anim Feed Sci Technol 2016;221:1-11. https://doi.org/10.1016/j.anifeedsci.2016.08.020
  10. Certel M, Erbas M, Uslu MK, Erbas MO. Effects of fermentation time and storage on the water-soluble vitamin contents of tarhana. J Sci Food Agric 2007;87:1215-8. https://doi.org/10.1002/jsfa.2810
  11. Ochanda SO, Akoth OC, Mwasaru MA, Kagwiria OJ, Mutiso MF. Effects of malting and fermentation treatments on group B-vitamins of red sorghum, white sorghum and pearl millets in Kenya. J Appl Biosci 2010;34:2128-34.
  12. Broderick GA, Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 1980;63:64-75. https://doi.org/10.3168/jds.S0022-0302(80)82888-8
  13. Playne MJ, McDonald P. The buffering constituents of herbage and of silage. J Sci Food Agric 1966;17:264-8. https://doi.org/10.1002/jsfa.2740170609
  14. Murphy RP. A method for the extraction of plant samples and the determination of total soluble carbohydrates. J Sci Food Agric 1958;9:714-7. https://doi.org/10.1002/jsfa.2740091104
  15. Association of Official Analytical Chemists (AOAC). Protein (crude) in animal feed and pet food 984.13. In: Cunniff PA, editor. Official Methods of Analysis of Official Analytical Chemists International, 16th edn volume I chapter 4. Arlington, VA, USA: AOAC International; 1995.
  16. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  17. Kilic A. Silo feed (instruction, education and application proposals). Izmir, Turkey: Bilgehan Press; 1986.
  18. IBM SPSS Statistics for Windows, version 19.0; Armonk, NY, USA; IBM Corp: 2010.
  19. Papadopoulos YA, Mckersie BD. A comparison of protein degradation during wilting and ensiling of six forage species. Can J Plant Sci 1983;63:903-12. https://doi.org/10.4141/cjps83-114
  20. Mckersie BD, Buchanan-Smith J. Changes in the levels of proteolytic enzymes in ensiled alfalfa forage. Can J Plant Sci 1982;62:111-6. https://doi.org/10.4141/cjps82-017
  21. Beaudet V, Gervais R, Graulet B, et al. Effects of dietary nitrogen levels and carbohydrate sources on apparent ruminal synthesis of some B vitamins in dairy cows. J Dairy Sci 2016;99:2730-9. https://doi.org/10.3168/jds.2015-10521
  22. Schwab EC, Schwab CG, Shaver RD, et al. Dietary forage and nonfiber carbohydrate contents influence B-vitamin intake, duodenal flow, and apparent ruminal synthesis in lactating dairy cows. J Dairy Sci 2006;89:174-87. https://doi.org/10.3168/jds.S0022-0302(06)72082-3
  23. Rao RV, Basu KP. Effect of dahi fermentation on the thiamine, riboflavin and nicotinic acid contents of milk. Indian J Dairy Sci 1952;5:1-8.
  24. Alm L. Effect of fermentation on B-vitamin content of milk in Sweden. J Dairy Sci 1982;65:353-9. https://doi.org/10.3168/jds.S0022-0302(82)82199-1
  25. Shahani KM, Chandan RC. Nutritional and healthful aspects of cultured and culture-containing dairy foods. J Dairy Sci 1979;62:1685-94. https://doi.org/10.3168/jds.S0022-0302(79)83481-5
  26. Capozzi V, Russo P, Duenas MT, Lopez P, Spano G. Lactic acid bacteria producing B-group vitamins: a great potential for functional cereals products. Appl Microbiol Biotechnol 2012;96:1383-94. https://doi.org/10.1007/s00253-012-4440-2
  27. Lindqvist H, Nadeau E, Jensen SK. Alpha-tocopherol and ${\beta}$-carotene in legume-grass mixtures as influenced by wilting, ensiling and type of silage additive. Grass Forage Sci 2012;67:119-28. https://doi.org/10.1111/j.1365-2494.2011.00827.x
  28. Tani Y, Tsumura H. Screening for tocopherol-producing microorganisms and ${\alpha}$-tocopherol production by Euglena gracilis Z. Agric Biol Chem 1989;53:305-12. https://doi.org/10.1080/00021369.1989.10869324

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