The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Long non-coding RNA T-cell leukemia/lymphoma 6 serves as a sponge for miR-21 modulating the cell proliferation of retinoblastoma through PTEN

  • Tao, Sisi (Department of Science and Education, Changsha Hospital for Maternal & Child Health Care) ;
  • Wang, Weidong (Department of Science and Education, Changsha Hospital for Maternal & Child Health Care) ;
  • Liu, Pengfei (Department of Orthopedics, Shaoyang County People's Hospital) ;
  • Wang, Hua (Department of Ophthalmology, The First Affiliated Hospital of Human Normal University/Hunan Provincial People's Hospital) ;
  • Chen, Weirong (Health Management Center, Changsha Hospital for Maternal & Child Health Care)
  • Received : 2018.08.11
  • Accepted : 2019.04.15
  • Published : 2019.11.01
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Abstract

Retinoblastoma (Rb) is one of the most common eye malignancies occur in childhood. The crucial roles of non-coding RNAs, particularly long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), have been widely reported in Rb progression. In the present study, we found the expression of lncRNA T-cell leukemia/lymphoma 6 (TCL6) was significantly downregulated in Rb tissues and cell lines. Knockdown of lncRNA TCL6 promoted cell proliferation while reduced cell apoptosis in Rb cells. Moreover, lncRNA TCL6 serves as a sponge for miR-21, a previously-reported oncogenic miRNA in Rb, by direct targeting to negatively regulated miR-21 expression, therefore modulating Rb proliferation through miR-21. TCL6 overexpression inhibited Rb cell proliferation while miR-21 overexpression exerted an opposing effect; the effect of TCL6 overexpression was partially attenuated by miR-21 overexpression. PTEN/PI3K/AKT signaling pathway was involved in lncRNA TCL6/miR-21 axis modulating Rb cell proliferation. Taken together, lncRNA TCL6 serves as a tumor suppressor by acting as a sponge for miR-21 to counteract miR-21-mediated PTEN repression.

Keywords

References

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