Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antidiabetic Effect of Ethanol Extract on Astragali Radix

황기 에탄올 추출물의 항 당뇨 효과

  • Kim, Ok-Kyung (Department of Food Science and Nutrition, Dae Jin University)
  • 김옥경 (대진대학교 과학기술대학 식품영양학과)
  • Received : 2019.08.19
  • Accepted : 2019.09.27
  • Published : 2019.09.30
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Abstract

This study was carried to investigate the antidiabetic effect of ethanol extract of Astragali Radix(A.R) in Streptozotocin(STZ) induced diabetic rats. Diabetes was induced by intravenous injection of STZ at a dose of 45mg/kg dissolved in citrate buffer. The ethanol extract of A. R was orally administrated once a day for 7 days at a dose of 1,000mg/kg. The contents of serum glucose, triglyceride(TG), total cholesterol were significantly decreased in A.R treated group compared to the those of STZ-control group. The content of hepatic glycogen and activities of glucokinase(GK) and glucose-6-phosphate dehydrogenase(G-6-PDH) were significantly increased, and activity of glucose-6-phoshatase(G-6-Pase) was significantly decreased in A.R treated group compared to the those of STZ-control group, These results indicated that ethanol extract of A.R would have antidiabetic effect in STZ-induced diabetic rats.

Streptozotocin(STZ)을 45mg/kg.b.w의 용량으로 흰쥐의 미정맥에 투여 한 후 당뇨병이 유발된 당뇨 흰쥐에게 1일 1회 7일간 1,000mg/kg의 용량으로 황기에탄올 추출물을 투여 후 glucose함량과 당대사에 관여하는 효소인 glucose-6-phosphatase(G-6-Pase), glucose-6-phosphate dehydrogenase(G-6-PDH, glucokinase(GK)활성과 glycogen 함량, triglyceride(TG), total cholesterol등의 지질대사에 관여하는 물질들을 측정한 결과 황기 에탄올 추출물 투여군이 glucose, TG, total cholesterol등의 함량과 G-6-Pase 활성의 유의적인 감소를 나타내었으며 glycogen 함량과 G-6-PDH, GK의 활성이 유의적인 증가를 나타내었다. 이와 같이 황기 에탄올 추출물이 항당뇨 개선효과를 갖는 유효성분을 함유하고 있음을 알 수 있었다.

Keywords

References

  1. J. H. Shin, S. J. Lee, J. K. Seo, H. J. Lee, J. C. Ju, N. J. Sung, "Effect of a Combined Exteact of Orostachyas japonicus with Medicinal Plants on the Lipid Composition of the Liver and Kidney from streprozotocin-induced Diabetic Rats", J. Ko. Soc. Food Sci Nutr. Vol.41, No.1, pp.510 (2012). https://doi.org/10.3746/jkfn.2012.41.4.510
  2. P. Daisy, K. Saipriya, "Biochemical analysis of cassia fistula aqueous extract and phytochemically sythesized gold nanoparticles as hypoglycemic treatment for diabetes mellitus." Int. J. nanomedicine, Vol.7, No.1, pp.1189 (2012).
  3. S. H. Choi., J. R. Park, "Lipid Modulatory Functions of Cysteine Compounds found in genus Allium plants in diabetic mice". Ko. J. Food Nutr. Vol.23, pp.361 (2010).
  4. T. Sun, Y. H. Chang, Uy J. Q, Uy, "Effect of Fu-zheng therapy in the management of diseases" Chin. Med. J. Vol 61, No.7, pp.97-101 (1981).
  5. JungYak Desajon. Sanghae science Pub. Sohakkwan, Tokyo, Vol.1, p.121 (1985).
  6. M. thomoda, N. Shimizu, N. Ohara, R. Gonda, S. Ishii and H. A. Otsuki "Reticuloendothelial system activiting Glycan from Roots of Astraglaus membranaceus". Phytochemistry, Vol,31, No1, pp.63-66 (1992). https://doi.org/10.1016/0031-9422(91)83006-7
  7. Y. D. Zang, Y. L. Wang, J. P. Shen and D.X. Li "Hypotensive and antiinflammatory effects of Astraglaus saponin" 1. Acta Pharm. Scin. Vol.19, No.8, pp.333-337 (1984).
  8. H. Hikino, S. Funayama and K.Endo, "hytotensive principles of Astraglaus and hedysarum roots". Planta Med. Vol.30, No.10, pp.297-302 (1976). https://doi.org/10.1055/s-0028-1097733
  9. E. J. Kim., O. J. O., S. K. Lee, and K. S. Yang. "Inhibitory effect of Astragali Radix on COX-2 activity", Kor. J. Pharmacogn, Vol.32, No.4, pp.311-315 (2001).
  10. J. Y. Lee, H. Y. Park, M. H. Teom, D. H. Kim and H. K. Kim. "The protective Effects of Astragali Radix against UV Induced cellular damage in Human Keratinocytes" Kor. J. Pharmacogn, Vol.39, No.4, pp.300-304 (2008).
  11. M. Hirotani, Y. zhow, Y. rui, T, Furuya, "Cycloartane triterpene Glycosides from the hexiry Root Cultures of Astraglaus mongholicus", Phytochemistry, Vol.37, No.5, pp.63-66 (1994).
  12. I. Kitagawa, H. K. Wang, M. Saito and M. Yoshikawa. "Saponin and Sapogenol XXX VI, chemical constituents the root of Astraglaus membranaceus Bunce(3) Astragalosides III, V and VI", Chem. Pharm. Bull. Vol.31, No.10, pp.709-715 (1983) https://doi.org/10.1248/cpb.31.709
  13. O. K. Kim, "Antidiabetic and antioxidative effects of Lycii fructus in streptorotocin Induced Diabetic Rats". Kor. J. Pharmacogn, Vol.40, Vol.2, pp.128-136 (2009).
  14. J. R, Williamson, K. Chang, M. Franges, K. S, Hasan, "Perspectives in diabetic hyperglycemic pseudohypoxia and diabetic complications". Diabetes, Vol. 42, No.8, pp.801 (1993).
  15. J. A, Manuel, M. J, Ayber, A. N, Riera, A. Grau, S. S. Sanchez, "Hypoglycemic effect of the water extract of Smallantus sonchifoius(yacon) leaves in normal and diabetic rats". Journal of Ethnopharmacology, Vol.74, No.10, pp.125 (2001). https://doi.org/10.1016/S0378-8741(00)00351-2
  16. X. Yan, S. Masashiro, M. Kameyama, Y. Sada, Y. Nakanishi, T. Nagata, "Extraction and Identification of antioxidants in the roots of yacon (Smallanthus sonchifolius)". J. Agric. Food Chem., Vol.47, No.6, pp.4711 (1999). https://doi.org/10.1021/jf981305o
  17. T. Sumio, K.Ito, A.Yoshimura, N.Noguchi, T. Ishida, "The constituents relate to anti-oxidative and ${\alpha}$-glucosidase inhibitory activities in yacon aerial part extract". yakugaku zasshi, Vol.126, No.4, pp.665 (2006). https://doi.org/10.1248/yakushi.126.665
  18. N. M. O,mera, R. bevery, O. collins, P. B, Johnson, A. H, Tomkin, G. H, Chol, "Metabolism in alloxan-induced diabetic rabbits". Diabetes, Vol.39, No.10, pp.626 (1990). https://doi.org/10.2337/diab.39.5.626
  19. S. Y. Cho, J. Y. Park, E. M, Park, M. S, Choi, M. K. Lee, S. M. Jeon, M. K, Jang, M. J, Kim, Y. B, Park, "Alternation of hepatic antioxidant enzyme activities and lipid profile in streptozotocin-induced diabetic rats by supplementation of dandelion water extract". Clin. Chim. Acta., Vol.317, No.10, pp.109 (2002). https://doi.org/10.1016/S0009-8981(01)00762-8
  20. B. M. Rinkind, "Diet plasma cholesterol and coronary heart disease", J. Nutrition, Vol.119, No.15, pp.1100 (1989). https://doi.org/10.1093/jn/119.8.1100
  21. R. B. Goldberg, "Lipid disorders in diabetes". Diabetes Care,, Vol.4, No.2, pp.561 (1981). https://doi.org/10.2337/diacare.4.5.561
  22. K. M. West, M. M. S, Ahuja, P. H. Bennett, "The role of circulating glucose and triglyceride concentration and their interaction with other "risk factor" as determinants of asterial disease in nine diabetic population samples from WHO multinational study". Diabetes Care, Vol.6, No.3, pp.361 (1983). https://doi.org/10.2337/diacare.6.4.361
  23. M. D. Meglasson, P. T. Burch, D. K. Berner, H. Najafi, F.M, Matschinsky, "Identification of glucokinase as an alloxan-sensitive glucose sensor of the pancreatic-cell". Diabetes Vol.35, No.2, pp.1163 (1986). https://doi.org/10.2337/diab.35.10.1163
  24. R. Ghosh, B. Mukherjee, M. A, Chatterjee, "A novel effect of selenium on streptozotocin induced diabetic mice". Diabetes Res., Vol.25, No.5, pp165 (1994).
  25. S. Himeno, A. Takekawa, N. Imura, "Special difference in hydroperoxide scavenging enzyme with special reference to glutathione peroxidase in guinea-pigs". Comp. Bodchem. Physiol. B., Vol.104, No.9, pp.27 (1993). https://doi.org/10.1016/0305-0491(93)90334-2