Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of a probiotic mixture on egg quality and egg production in laying hens

복합생균제가 산란계의 생산성, 계란품질 및 악취저감에 미치는 영향

  • Shin, JS (College of Animal Life Sciences, Kangwon National University) ;
  • Um, KH (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, JY (College of Animal Life Sciences, Kangwon National University) ;
  • Choi, YS (College of Animal Life Sciences, Kangwon National University) ;
  • Park, HJ (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, HS (College of Animal Life Sciences, Kangwon National University) ;
  • Park, BS (College of Animal Life Sciences, Kangwon National University)
  • 신종서 (강원대학교 동물생명과학대학) ;
  • 엄경환 (강원대학교 동물생명과학대학) ;
  • 이정윤 (강원대학교 동물생명과학대학) ;
  • 최윤서 (강원대학교 동물생명과학대학) ;
  • 박희진 (강원대학교 동물생명과학대학) ;
  • 이혜선 (강원대학교 동물생명과학대학) ;
  • 박병성 (강원대학교 동물생명과학대학)
  • Received : 2019.08.05
  • Accepted : 2019.09.21
  • Published : 2019.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of the present study was to evaluate effects of probiotic mixture (PM) including Bacillus subtillus, Streptomyces galilaeus and Sphingobacteriaceae on egg production, egg quality, and eliminating odor in laying hens. A total of 240 Hy-Line Brown laying hens (50 wks of age) were divided into four treatment groups (60 replicates of birds, each) in completely randomized design. Groups were assigned to four experimental diets: CON (a basal diet of no PM additive as control), basal diet supplemented with either CP3 (0.3% commercial probiotics), PM3 (0.3% PM), or PM5 (0.5% PM 0.5). Data of egg production and egg quality were obtained during 6 weeks of experimental period. Egg production, Haugh unit, shell thickness, breaking strength, yolk color, and albumin high were significantly increased in CP3 and PM3 compared to those in CON and CP3. Ammonia levels and numbers of E. coli, total aerobic bacteria, Coliform, and Salmonella in feces were significantly increased in order of CON>CP3>PM3>PM5. The present study indicates that addition of 0.3% probiotic mixture including B. subtillus, S. galilaeus and Sphingobacteriaceae to diets may improve egg production, egg quality, and eliminate fecal ammonia of laying hens.

본 연구는 계란 생산성, 계란품질 및 악취저감에 관한 Bacillus subtillus, Streptomyces galilaeus 및 Sphingobacteriaceae로 구성된 복합생균제(PM, probiotic mixture)의 효과를 조사하였다. 산란계 Hy-Line Brown 240수를 4처리구로 구분하여 6주 동안 사육하였다. 처리구는 복합생균제를 함유하지 않은 대조구(CON, control), 일반생균제(CP3, commercial probiotics 0.3%), 복합생균제 3(PM3, 복합생균제 0.3%), 복합생균제 5(PM5, 복합생균제 0.5%)로 구분하였다. 산란율, 계란 호우유니트, 난각두께, 난각강도, 난황색 및 농후난백 높이는 PM3, PM5가 CON 및 CP3에 비해서 높았다. 계분으로부터 암모니아, E. coli, 총호기성균, Coliform, Salmonella 균수는 CON>CP3>PM3>PM5 순서로 증가하였다. 본 결과는 B. subtillus, S. galilaeus 및 Sphingobacteriaceae로 구성된 복합생균제 0.3%가 산란율, 계란품질 개선 및 분에서 암모니아 발생을 낮추어 양계장 악취저감 효과를 갖는다는 점을 보여준다.

Keywords

References

  1. A. C. Ouwehand, S. Salminen, E. Isolauri, "Probiotics: an overview of beneficial effects", Antonie van Leeuwenhoek, Vol.82, pp. 279-289, (2002). https://doi.org/10.1023/A:1020620607611
  2. F. W. Edens, "An alternative for antibiotic se in poultry: probiotics", Brazilian Journal of Poultry Science, Vol.5, No.2 pp. 75-97, (2003). https://doi.org/10.1590/S1516-635X2003000200001
  3. G. R. Ross, C. Gusils, R. Oliszewski, S. C. De Holgado, S. N. Gonzalez, "Effects of probiotic administration in swine", J. Biosci. Bioengin, Vol.109, No.6 pp. 545-549, (2010). https://doi.org/10.1016/j.jbiosc.2009.11.007
  4. T. Balevi, U. S. Ucan, B. Cosun, V. Kurtogu, I. S. Cetingul, "Effect of dietary probiotic on performance and humoral immune response in layer hens", Brit. Poult. Sci, Vol.42, No.4 pp. 456-461, (2001). https://doi.org/10.1080/00071660120073133
  5. C. Toms, F. Powrie, "Control of intestinal inflammation by regulatory T cells", Microbes Infection, Vol.3, No.11 pp. 929-935, (2001). https://doi.org/10.1016/S1286-4579(01)01454-X
  6. A. Sullivan, C. E. Nord, "Probiotics and gastrointestinal diseases", J. Int. Med, Vol.257, No.1 pp. 78-92, (2005). https://doi.org/10.1111/j.1365-2796.2004.01410.x
  7. S. E. Higgins, J. P. Higgins, A. D. Wolfenden, S. N. Henderson, A. Torres-Rodriguez, G. Tellez, B. Hargis, "Evaluation of a Lactobacillus-based probiotic culture for the reduction of Salmonella enteritidis in neonatal broiler chicks", Poult. Sci, Vol.87, No.1 pp. 27-31, (2008). https://doi.org/10.3382/ps.2007-00210
  8. J. S. Kim, S. L. Ingale, Y. W. Kim, K. H. Kim, S. Sen, M. H. Ryu, J. D. Lohakare, I. K. Kwon, B. J. Chae, "Effect of supplementation of multi-microbe probiotic product on growth performance, apparent digestibility, cecal microbiota and small intestinal morphology of broilers", J. Anim. Physiol. Anim. Nutr, Vol.96, No.4 pp. 618-626, (2012). https://doi.org/10.1111/j.1439-0396.2011.01187.x
  9. S. Forssten, M. Evans, D. Wilson, A. C. Ouwehand, "Influence of a probiotic mixture on antibiotic induced microbiota disturbances", World J. Gastroenterol, Vol.20, No.33 pp. 11878-11885, (2014). https://doi.org/10.3748/wjg.v20.i33.11878
  10. D. H. Nguyen, C. M. Nyachoti, I. H. Kim, "Evaluation of effect of probiotics mixture supplementation on growth performance, nutrient digestibility, faecal bacterial enumeration, and noxious gas emission in weaning pigs", Italian J. Anim. Sci, Vol.18, No.1 pp. 466-473, (2019). https://doi.org/10.1080/1828051X.2018.1537726
  11. S. M. Kabir, "The role of probiotics in the poultry industry". Int. J. Molecul. Sci, Vol.10, No.8 pp. 3531-3546, (2009) https://doi.org/10.3390/ijms10083531
  12. H. H. Giang, T. Q. Viet, B. Ogle, J. E. Lindberg, "Effects of different probiotic complexes of lactic acid bacteria on growth performance and gut environment of weaned piglets", Livestock Sci, Vol.133, No.1-3 pp. 182-184, (2010). https://doi.org/10.1016/j.livsci.2010.06.059
  13. NRC, Guide for the care and use of laboratory animals. Eighth Ed. The national academies press. Washington D.C. USA. (2011a).
  14. NRC. Nutrients requirements of poultry. 9th rev. National Academy Press, Washington DC, (2001b).
  15. S. O. Park, B. S. Park, "Effects of dietary inuloprebiotics on egg production and on the microbial ecology and blood lipid profile of laying hens", J. Life Sci, Vol.22, No.7 pp. 880-888, (2012). https://doi.org/10.5352/JLS.2012.22.7.880
  16. S. O. Park, J. Hwangbo, B. S. Park, H. C. Choi, "Effects of inverse lighting and extreme heat diet on short chain fatty acid and blood lipid profile in extreme heat stress-exposed broilers", J. Korean Oil Chemists Society, Vol.30, No.3 pp. 400-410, (2013) https://doi.org/10.12925/jkocs.2013.30.3.400
  17. C. L. Xu, C. Ji, Q. Ma, K. Hao, Z. Y. Jin, K. Li, "Effects of a dried Bacillus subtilis culture on egg quality", Poult. Sci, Vol.85, No.2 pp. 364-368, (2006). https://doi.org/10.1093/ps/85.2.364
  18. M. Gnanadesigan, S. Isabella, P. Saritha, L. Ramkumar, N. Manivannan, R. Ravishankar, "Quality evaluation of egg composition and productivity of layers in EM (Effective Microorganisms) treatments: A field report", Egyptian J. Basic Appl. Sci, Vol.1, No.3-4 pp. 161-166, (2014). https://doi.org/10.1016/j.ejbas.2014.06.003
  19. L. V. Hooper, J. I. Gordon, "Commensal host-bacterial relationships in the gut", Science, Vol.292, No.5519 pp. 1115-1118, (2001). https://doi.org/10.1126/science.1058709
  20. H. Tlaskalova-Hogenova, R. Stepankova, T. Hudcovic, L. Tuckova, B. Cukrowska, R. Lodinova-Zadnikova, H. Kozakova, P. Rossmann, J. Bartova, D. Sokol, D. P. Funda, D. Borovska, Z. Rehakova, J. Sinkora, J. Hofman, P. Drastich, A. Kokesova, "Commensal bacteria (normal microflora), mucosal immunity and chronic inflammatory and autoimmune diseases", Immun. Letters, Vol.93, No.2-3 pp. 97-108, (2004). https://doi.org/10.1016/j.imlet.2004.02.005
  21. C. Alexopoulos, I. E. Georgoulakis, A. Tzivara, S. K. Kritas, A. Siochu, S. C Kyriakis, "Field evaluation of the efficacy of a probiotic containing Bacillus licheniformis and Bacillus subtilis spores, on the health status and performance of sows and their litters", J. Anim. Physiol. Anim. Nutr, Vol.88, No.11-12 pp. 381-392, (2004). https://doi.org/10.1111/j.1439-0396.2004.00492.x
  22. H. M. Jayaprakasha, Y. C. Yoon, H. D. Paik, "Probiotic Functional Dairy Foods and Health Claims", Food Sci. Biotech, Vol.14, No.4 pp. 523-528, (2005).
  23. M. Aalaei, A. Khatibjoo, M. Zaghari, K. Taherpour, M. Soltani, "Comparison of single-and multi-strain probiotics effects on broiler breeder performance, egg production, egg quality and hatchability", Brit. Poult. Sci, Vol.59, No.5 pp. 531-538, (2018). https://doi.org/10.1080/00071668.2018.1496400
  24. Y. C. Chen, T. C. Chen, "Effects of commercial probiotic or prebiotic supplementation on production, size and quality of hens egg", Poult. Sci, Vol.82, No.1 pp.330-(2003).
  25. V. Garcia, P. Catala-Gregori, F. Hernandez, M. D. Megias, J. Madrid, "Effect of formic acid and plant extracts on growth, nutrient digestibility, intestine mucosa morphology, and meat yield of broilers", J. Appl. Poult. Res, Vol.16, No.4 pp. 555-562, (2007). https://doi.org/10.3382/japr.2006-00116
  26. R. D. Rolfe, "The role of probiotic cultures in the control of gastrointestinal health", J. Nutr, Vol.130, No.2 pp. 396S-402S, (2000). https://doi.org/10.1093/jn/130.2.396S
  27. W. F. Zhang, D. F. Li, W. Q. Lu, G. F. Yi, "Effects of isomalto-oligosaccharides on broiler performance and intestinal microflora", Poult. Sci, Vol.82, No.4 pp. 657-663(2003). https://doi.org/10.1093/ps/82.4.657
  28. S. Devaraj, S. Vega-Lopez, N. Kaul, F. Schonlau, P. Rohdewald, I. Jialal, "Supplementation with a pine bark extract rich in polyphenols increases plasma antioxidant capacity and alters the plasma lipoprotein profile", Lipids, Vol.37, No.10 pp. 931-934, (2002). https://doi.org/10.1007/s11745-006-0982-3
  29. E. Tako, R. P. Glahn, R. M. Welch, X. Lei, K. Yasuda, D. D. Miller, "Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine", Brit. J. Nutr, Vol.99, No.3 pp. 472-480, (2008). https://doi.org/10.1017/S0007114507825128
  30. B. S. Park, "Bifidogenic effects of inuloprebiotics in broiler chickens", J. Life Sci, Vol.18, No.12 pp. 1693-1699, (2008). https://doi.org/10.5352/JLS.2008.18.12.1693
  31. J. Ahn, I. U. Grun, A. Mustapha, "Effects of plant extracts on microbial growth, color change, and lipid oxidation in cooked beef", Food microbiology, Vol.24, No.1 pp. 7-14, (2007). https://doi.org/10.1016/j.fm.2006.04.006
  32. C. Forte, G. Acuti, E. Manuali, P. Casagrande Proietti, S. Pavone, M. Trabalza-Marinucci, L. Moscati, A. Onofri, C. Lorenzetti, M. P. Franciosini, "Effects of two different probiotics on microflora, morphology, and morphometry of gut in organic laying hens", Poult. Sci, Vol.95, No.11 pp. 2528-2535, (2013). https://doi.org/10.3382/ps/pew164
  33. M. Rodrigues, P. C. Pozza, M. S. S. Pozza, M. Possamai, L. D. G. Bruno, E. Richart, M. O. Wochner, J. M. Pereira Junior, "Effects of inulin and a probiotic mixture on nutrient digestibility and nitrogen balance in piglets", Arch. Zootec, Vol.62, No.238 pp. 255-264, (2013). https://doi.org/10.4321/S0004-05922013000200011
  34. W. Yanbo, X. Zirong, "Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities", Anim. Feed Sci. Technol, Vol.127, No.3-4 pp. 283-292, (2006). https://doi.org/10.1016/j.anifeedsci.2005.09.003
  35. H. H. Giang, T. Q. Viet, B. Ogle, J. E. Lindberg, "Effects of supplementation of probiotics on the performance, nutrient digestibility and faecal microflora in growing-finishing pigs". AAJAS, Vol.24, No.5 pp. 655-661, (2011).
  36. P. A. Adhikari, W. K. Kim, "Overview of prebiotics and probiotics: focus on performance, gut health and immunity-a review", Annals Anim. Sci, Vol.17, No.4 pp. 949.966, (2017). https://doi.org/10.1515/aoas-2016-0092
  37. J. J. Mallo, J. Rioperez, P. Honrubia, "The addition of Enterococcus faecium to diet improves piglet's intestinal microbiota and performance", Livestock Sci, Vol.133, No.1-3 pp. 176-178, (2010). https://doi.org/10.1016/j.livsci.2010.06.057