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The Korean Society of Phycology (한국조류학회(藻類))
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Intraspecific variations in macronutrient, amino acid, and fatty acid composition of mass-cultured Teleaulax amphioxeia (Cryptophyceae) strains

  • Lee, Bae Ik (Aquaculture Research Department, National Institute of Fisheries Science) ;
  • Kim, Shin Kwon (Aquaculture Research Department, National Institute of Fisheries Science) ;
  • Kim, Jong Hyeok (Dinorena Co. Ltd.) ;
  • Kim, Hyung Seop (Department of Marine Biotechnology, Kunsan National University) ;
  • Kim, Jong Im (Department of Biology, Chungnam National University) ;
  • Shin, Woongghi (Department of Biology, Chungnam National University) ;
  • Rho, Jung-Rae (Department of Marine Biotechnology, Kunsan National University) ;
  • Yih, Wonho (Department of Marine Biotechnology, Kunsan National University)
  • Received : 2019.02.28
  • Accepted : 2019.06.04
  • Published : 2019.06.15
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Abstract

To compare the nutritional quality of TPG (Teleaulax / Plagioselmis / Geminigera) clade species of cryptomonads with that of RHO (Rhodomonas / Rhinomonas / Storeatula) clade species 6 Teleaulax amphioxeia (TA) and 1 Rhinomonas sp. strains were mass-cultured in newly designed 500-L photobioreactors to the end of exponential growth phase. Intraspecific variations (IVs) in terms of one standard deviation among the 6 TA strains in the compositions of the three macronutrients were 41.5 (protein), 89.8 (lipid), and 15.6% (carbohydrate) of the mean. When harvested from stationary growth phase mean compositions of essential amino acids (EAAs, 47.3%) and non-EAAs (52.7%) of the 2 TA strains, CR-MAL07 and CR-MAL08-2, were similar to those of a Chroomonas strain. The IVs between the 2 TA strains in the composition of EAAs (10.3 and 2.4) and non-EAAs (8.5 and 2.1% of the mean) were rather smaller than those of saturated fatty acids (30.3 and 26.1) and unsaturated fatty acids (UFAs, 12.0 and 12.5% of the mean) in f/2-Si and urea-based compound fertilizer (UCF) culture media, respectively. Mean compositions of eicosapentaenoic acid (EPA, 17.9%) and docosahexaenoic acid (DHA, 12.7%) of total fatty acids of the 2 TA strains were higher than those that of a Chroomonas strain. EPA and DHA compositions exhibited similar level of IVs between the 2 TA strains in f/2-Si (14.6 and 11.0) and UCF media (12.6 and 13.5% of the mean). Thus, the nutritional quality in terms of amino acids, UFAs, EPA, and DHA in a TPG clade species, T. amphioxeia was comparable to those of RHO clade species with notable IVs. Practically, biotechnological targets for TPG clade cryptomonad strains might be subspecies or clone level.

Keywords

Acknowledgement

Grant : Mass-production of multifunctional aquaculture live-feeds in renovative photobioreactors

Supported by : National Institute of Fisheries Science (NIFS), KIMST (Korea Institute of Marine Science and Technology), NRF

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