Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Relationship between ganglioside expression and anti-cancer effects of a plant-derived antibody in breast cancer cells

  • Ju, Won Seok (Department of Biological science, College of Natural Sciences, Wonkwang University) ;
  • Song, Ilchan (Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University) ;
  • Park, Se-Ra (Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University) ;
  • Seo, Sang Young (Department of Biological science, College of Natural Sciences, Wonkwang University) ;
  • Cho, Jin Hyoung (Department of Biological science, College of Natural Sciences, Wonkwang University) ;
  • Min, Sung-Hun (Center of Reproductive Medicine, Good Moonhwa Hospital) ;
  • Kim, Dae-Heon (Department of Biology, Sunchon National University) ;
  • Kim, Ji-Su (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Sun-Uk (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Soon Ju (Department of Biological science, College of Natural Sciences, Wonkwang University) ;
  • Ko, Kisung (Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University) ;
  • Choo, Young-Kug (Department of Biological science, College of Natural Sciences, Wonkwang University)
  • Received : 2019.08.29
  • Accepted : 2019.09.04
  • Published : 2019.09.30
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Abstract

Production of therapeutic monoclonal antibodies (mAbs) using a plant platform has been considered an alternative to the mammalian cell-based production system. A plant-derived mAb CO17-1AK ($mAb^P$ COK) can specifically bind to various types of cancer cell lines. The target protein of $mAb^P$ COK is the epithelial cell adhesion molecule (EpCAM) highly expressed in human epithelial cancer cells, including breast and colorectal cancer cells. It has been hypothesized that its overexpression supports tumor growth and metastasis. A ganglioside is extended well beyond the surfaces of the various cell membranes and has roles in cell growth, inflammation, differentiation, and carcinogenesis. However, the regulation of EpCAM gene expression in breast cancers and the role of gangliosides in oncogenesis are unclear. Here, the purpose of this study was to determine the effects of $mAb^P$ COK on human breast cancer cell proliferation, apoptosis, and ganglioside expression patterns. Our results show that treatment with $mAb^P$ COK suppressed the growth of breast cancer cells and induced apoptotic cell death. It also upregulated the expression of metastasis-related gangliosides in breast cancer cells. Thus, treatment with $mAb^P$ COK may have chemo-preventive therapeutic effects against human breast cancer.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF), KRIBB, System and Synthetic Agrobiotech Center

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