The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein

  • Choi, Go-Woon (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Oh, June-Pyo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Ju, Hye-Kyoung (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Hu, Wen-Xing (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Boram (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo, Eun-Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Park, Jong-Seok (Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Domier, Leslie L (Department of Crop Sciences, University of Illinois at Urbana-Champaign) ;
  • Hammond, John (United States Department of Agriculture-Agricultural Research Service, Floral and Nursery Plants Research Unit) ;
  • Song, Kihak (Department of Urology, Chungnam National University School of Medicine) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 투고 : 2018.12.10
  • 심사 : 2019.06.10
  • 발행 : 2019.10.01
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초록

In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.

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참고문헌

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