The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification of Differentially Up-regulated Genes in Apple with White Rot Disease

  • Kang, Yeo-Jin (Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University) ;
  • Lee, Young Koung (Cold Spring Harbor Laboratory) ;
  • Kim, In-Jung (Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University)
  • Received : 2018.10.09
  • Accepted : 2019.06.27
  • Published : 2019.10.01
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Abstract

Fuji, a major apple cultivar in Korea, is susceptible to white rot. Apple white rot disease appears on the stem and fruit; the development of which deteriorates fruit quality, resulting in decreases in farmers' income. Thus, it is necessary to characterize molecular markers related to apple white rot resistance. In this study, we screened for differentially expressed genes between uninfected apple fruits and those infected with Botryosphaeria dothidea, the fungal pathogen that causes white rot. Antimicrobial tests suggest that a gene expression involved in the synthesis of the substance inhibiting the growth of B. dothidea in apples was induced by pathogen infection. We identified seven transcripts induced by the infection. The seven transcripts were homologous to genes encoding a flavonoid glucosyltransferase, a metallothionein-like protein, a senescence-induced protein, a chitinase, a wound-induced protein, and proteins of unknown function. These genes have functions related to responses to environmental stresses, including pathogen infections. Our results can be useful for the development of molecular markers for early detection of the disease or for use in breeding white rotresistant cultivars.

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References

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