Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지
DOI QR코드

DOI QR Code

Morphology, Histology, and Histochemistry of the Olfactory Organ in Korean Endemic Fish, Rhodeus uyekii (Pices, Cyprinidae)

한국고유종 각시붕어 Rhodeus uyekii 후각기관의 형태 및 조직, 세포화학적 특성 연구

  • Kim, Hyun Tae (Department of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University) ;
  • Yun, Seung Woon (Department of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University) ;
  • Lee, Yong Joo (Jeonju National University of Education) ;
  • Park, Jong Young (Department of Biological Science and Institute for Biodiversity Research, College of Natural Sciences, Chonbuk National University)
  • 김현태 (전북대학교 자연과학대학 생명과학과, 전북대학교 부설 생물다양성연구소) ;
  • 윤승운 (전북대학교 자연과학대학 생명과학과, 전북대학교 부설 생물다양성연구소) ;
  • 이용주 (전주교육대학교 과학교육과) ;
  • 박종영 (전북대학교 자연과학대학 생명과학과, 전북대학교 부설 생물다양성연구소)
  • Received : 2019.07.03
  • Accepted : 2019.08.14
  • Published : 2019.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The olfactory organ of the Korean endemic fish, Rhodeus uyekii, a mussel-spawning species, was researched anatomically, histologically and histochemically, for looking into a relation to the physical and chemical condition of its habitat and ecological habit, using stereo microscopy, light microscopy and scanning electron microscopy. The external structure of the olfactory organ consists of the semicircular-shaped anterior nostril with arched shape at its forward position, posterior nostrils and the nasal flap. Within the olfactory chamber, it has the rosette structure with 14 to 15 lamellae which is largely divided into the sensory and non-sensory regions. The sensory region has the olfactory receptor neurons, the supporting cells, the basal cells, the lymphatic cells, and the plasma cells, while the non-sensory region has the stratified epithelial cells, the mucous cells with sulfomucin and 1 type of unidentified cell. In particular, the arched feature in the anterior nostril and the mucous cell of sulfomucin were unique.

한국 고유종 각시붕어 Rhodeus uyekii의 후각기관과 서식처의 물리 및 화학적 특성 그리고 생태적 습성과의 연관성을 알아보기 위해 실체현미경, 광학현미경, 주사전자현미경을 이용하여 해부 및 조직, 세포화학적 특성을 조사하였다. 후각기관의 외부형태는 반원의 전비공과 후비공, 비경계판으로 구성되는데, 특히 전비공의 앞쪽에는 아치형의 조직이 돌출되어 있다. 비강 내에는 14~15개의 후판으로 구성되는 로제트 구조를 보유하며 후판은 감각상피와 비감각상피로 구분된다. 감각상피는 후감각뉴런, 지지세포, 기저세포, 림프구, 형질세포로 구성되는 반면에 비감각세포는 층상상피세포, 점액세포, 비감각섬모세포, 미확인세포가 1종류 확인된다. 점액세포의 세포화학적 특성은 산성 및 중성의 황점액소(sulfomucine)로 나타났다.

Keywords

References

  1. An, C.M. 1995. Effects of photoperiod and water temperature on the reproductive cycle of the spring-spawning bitterling, Rhodeus uyekii. Korean J. Ichthyol., 7: 43-55. (in Korean)
  2. Atta, K.I. 2013. Morphological, anatomical and histological studies on the olfactory organs and eyes of teleost fish: Anguilla anguilla in relation to its feeding habits. J. Basic Appl. Zool., 66: 101-108. https://doi.org/10.1016/j.jobaz.2013.10.002
  3. Bhute, Y.V. and V.V. Baile. 2007. Organization of the olfactory system of the Indian Major Carp Labeo rohita (Hamilton): a scanning and transmission electron microscopic study. J. Evol. Biochem. Physiol., 43: 342-349. https://doi.org/10.1134/S002209300703009X
  4. Bone, Q. and R. Moore. 2008. Biology of fishes. (3rd ed.) Taylor & Francis Group Pub., Milton Park, Abingdon, New York. 478p.
  5. Chae, B.S. 2001. Elongation of the ovipositor in Korean rose bitterling, Rhodeus uyekii (Pisces: Cyprinidae). Korean J. Ichthyol., 13: 111-116. (In Korean)
  6. Doving, K.B., M. Dubois-Dauphin, A. Holley and F. Jourdan. 1977. Functional anatomy of the olfactory organ of fish and the ciliary mechanism of water transport. Acta Zool., 58: 245-255. https://doi.org/10.1111/j.1463-6395.1977.tb00260.x
  7. Fanta, E. and C.A.O. Ribeiro. 2000. Microscopic morphology and histochemistry of the digestive system of a tropical fresh-water fish Trichomycterus brasiliensis (Lütken) (Siluroidei, Trichomycteridae). Revista Brasil. Zool., 17: 953-971. https://doi.org/10.1590/S0101-81752000000400007
  8. Frenkel, E.S. and K. Ribbeck. 2015. Salivary mucins protect surfaces from colonization by cariogenic bacteria. Appl. Environ. Microbiol., 81: 332-338. https://doi.org/10.1128/AEM.02573-14
  9. Ghosh, S.K. and P. Chakrabarti. 2013. Studies on the morphology of the olfactory organ in the freshwater teleost, Labeo bata (Hamilton). Mesopot. J. Mar. Sci., 28: 163-174.
  10. Ghosh, S.K. and P. Chakrabarti. 2014. Structural characterization of the olfactory epithelium of freshwater olive barb, Puntius sarana (Hamilton, 1822). Int. J. Aquat. Biol., 2: 147-154.
  11. Gurr, G.T. 1956. A practical manual of medical and biological staining techniques. Interscience, New York, pp. 1-99.
  12. Hansen, A., H.P. Zippel, P.W. Sorensen and J. Caprio. 1999. Ultrastructure of the olfactory epithelium in intact, axotomized, and bulbectomized goldfish, Carassius auratus. Micros. Res. Tech., 45: 325-338. https://doi.org/10.1002/(SICI)1097-0029(19990515/01)45:4/5<325::AID-JEMT16>3.0.CO;2-V
  13. Hansen, A. and B.S. Zielinski. 2005. Diversity in the olfactory epithelium of bony fishes: development, lamellar arrangement, sensory neuron cell types and transduction components. J. Neurocytol., 34: 183-208. https://doi.org/10.1007/s11068-005-8353-1
  14. Hara, T.J. 1986. Role of olfaction in fish behaviour. In: Pitcher, T.J. (ed.), The Behaviour of Teleost Fishes. Springer, US, pp. 152-176.
  15. Hara, T.J. 1994. Olfaction and gustation in fish: an overview. Acta Physiol. Scand., 152: 207-217. https://doi.org/10.1111/j.1748-1716.1994.tb09800.x
  16. Jeon, H.B. and H.Y. Suk. 2012. Male colors and female mate preference in Korean rosy bitterling, Rhodeus uyekii (Cyprinidae: Acheilognathinae). Korean J. Ichthyol., 24: 263-271. (in Korean)
  17. Kasumyan, A.O. 2004. The olfactory system in fish: structure, function, and role in behavior. J. Ichthyol., 44: S180.
  18. Kim, B.S., E.J. Kang, H. Jang and I.S. Park. 2012. Morphometric traits and cytogenetic analysis in induced cross and reciprocal hybrid between Rhodeus uyekii and R. notatus. Korean J. Ichthyol., 24: 151-159. (in Korean)
  19. Kim, C.H., W.O. Lee, Y.J. Kang and J.M. Baek. 2010. Occurrence of a natural intergeneric hybrid, Rhodeus uyekiixAcheilognathus signifer (Pisces; Cyprinidae) from Jojongcheon Bukhan River, Korea. Korean J. Ichthyol., 22: 225-229. (In Korean)
  20. Kim, D.M., H.B. Jeon and H.Y. Suk. 2014. Tanakia latimarginata, a new species of bitterling from the Nakdong River, South Korea (Teleostei: Cyprinidae). Ichthyol. Explor. Freshwaters, 25: 59-68.
  21. Kim, H.S., J.G. Ko, W.S. Choi and J.Y. Park. 2015. Population ecology of Korean rose bitterling, Rhodeus uyekii (Pisces: Acheilognathinae) in the Bong-seocheon, Mankyeonggang (river), Korea. Korean J. Ichthyol., 27: 78-85. (in Korean)
  22. Kim, H.T. 2018. A comparative anatomy and histology of the olfactory organ of 17 fishes in South Korea. Ph. D. dissertation, Chonbuk national university, South Korea, 175pp.
  23. Kim, H.T., Y.J. Lee and J.Y. Park. 2016. An anatomical and histochemical study of the olfactory organ in rice-fish Oryzias sinensis (Pisces: Adrianichthyidae) in South Korea. Korean J. Ichthyol., 28: 223-228.
  24. Kim, H.T., Y.J. Lee and J.Y. Park. 2018a. Functional anatomy and histology of the olfactory organ in Korean Eel Goby, Odontamblyopus lacepedii (Pisces: Gobiidae). Appl. Microsc., 48: 11-16. https://doi.org/10.9729/AM.2018.48.1.11
  25. Kim, H.T., Y.J. Lee, H.S. Kim and J.Y. Park. 2018b. Structure and histological characters of the olfactory organ in Korean endemic fish, Microphysogobio yaluensis (Cypriniformes, Cyprinidae). Korean J. Ichthyol., 30: 161-166. (in Korean) https://doi.org/10.35399/ISK.30.3.5
  26. Kim, I.J. 1997. A osteological study of Rhodeus uyekii. Korean J. Ichthyol., 9: 130-140. (in Korean)
  27. Kim, I.S. and J.Y. Park. 2002. Freshwater fishes of Korea. Kyo-Hak Publishing Co. Ltd., Korea, pp. 66-67. (in Korean)
  28. Kim, J.G. and J.Y. Park. 2016. A comparative study on the visual cells in two Korean bittering fishes. Korean J. Ichthyol., 28: 67-71. (in Korean)
  29. Lahnsteiner, F. and R.A. Patzner. 1990. The spermatic duct of blenniid fish (Teleostei, Blenniidae): fine structure, histochemistry and function. Zoomorphology, 110: 63-73. https://doi.org/10.1007/BF01632813
  30. Lauder, G.V. 1982. Patterns of evolution in the feeding mechanism of actinopterygian fishes. American Zool., 22: 275-285. https://doi.org/10.1093/icb/22.2.275
  31. Lavis, L.D. 2011. Histochemistry: live and in color. J. Histochem. Cytochem., 59: 139-145. https://doi.org/10.1369/0022155410395760
  32. Masson, P. 1929. Some histological methods. Trichrome stainings and their preliminary technique. J. Tech. Methods, 12: 75-90.
  33. Nakanishi, T., U. Fischer, J.M. Dijkstra, S. Hasegawa, T. Somamoto, N. Okamoto and M. Ototake. 2002. Cytotoxic T cell function in fish. Dev. Comp. Immunol., 26: 131-139. https://doi.org/10.1016/S0145-305X(01)00055-6
  34. Ojha, P.P. and A.S. Kapoor. 1973. Structure and function of the olfactory apparatus in the freshwater carp, Labeo rohita (Ham. Buch.). J. Morphol., 140: 77-85. https://doi.org/10.1002/jmor.1051400107
  35. Reid, S.D. 1995. Adaptation to and effects of acid water on the fish gill. In: Biochemistry and Molecular Biology of Fishes. Elsevier, Amsterdam, pp. 213-227.
  36. Samajdar, I. and D. Mandal. 2016. Histological organization and ultrastructures of the apical surface of the olfactory epithelium of a carp, Labeo bata (Hamilton). Int. J. Pure Appl. Zool., 4: 134-141.
  37. Smith, D.G. and K.E. Hartel. 1999. Margaritiferidae mollusca: Unionoida: possible hosts for Rhodeus (Pisces: Cyprinidae). Pol. Arch. Hydrobiol., 46: 277-281.
  38. Suzuki, N. and S.R. Jeon. 1988. Hybridization experiments in Acheilognathine fishes (Cyprinidae), an intrageneric hybrid between Rhodeus uyekii and R. ocellatus smithi. Korean J. Lim., 21: 129-142. (in Korean)
  39. Thornhill, R.A. 1967. The ultrastructure of the olfactory epithelium of the lamprey Lampetra fluviatilis. J. Cell Sci., 2: 591-602. https://doi.org/10.1242/jcs.2.4.591
  40. Varute, A.T. and S.K. Jirge. 1971. Histochemical analysis of mucosubstances in oral mucosa of mouthbreeding cichlid fish and seasonal variations in them. Histochem., 25: 91-102. https://doi.org/10.1007/BF00303950
  41. Wang, X., G.L. Hao, B.Y. Wang, C.C. Gao, Y.X. Wang, L.S. Li and J.D. Xu. 2019. Function and dysfunction of plasma cells in intestine. Cell Biosci., 9: 26. https://doi.org/10.1186/s13578-019-0288-9
  42. Whiteley, A.R. 2007. Trophic polymorphism in a riverine fish: morphological, dietary, and genetic analysis of mountain whitefish. Biol. J. Linn. Soc., 92: 253-267. https://doi.org/10.1111/j.1095-8312.2007.00845.x
  43. Yamamoto, M. 1982. Comparative morphology of the peripheral olfactory organs in teleosts. In: Hara, T.J. (ed.), Chemoreception in Fishes. Elsevier, Amsterdam, pp. 39-59.
  44. Zeiske, E., J. Kux and R. Melinkat. 1976. Development of the olfactory organ of oviparous and viviparous cyprinodonts (Teleostei). Zeit. Zool. Syst. Evolut., 14: 34-40. https://doi.org/10.1111/j.1439-0469.1976.tb00516.x
  45. Zeiske, E., B. Theisen and H. Breucher. 1992. Structure, development, and evolutionary aspects of the peripheral olfactory system. In: Hara, T.J. (ed.), Fish Chemoreception. Springer, Netherlands, pp. 13-39.