Journal of Animal Science and Technology

  • 제61권5호
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  • Pages.245-253
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  • 2019
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  • 2672-0191(pISSN)
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  • 2055-0391(eISSN)
한국축산학회 (Korean Society of Animal Sciences and Technology)
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Natural and synthetic pathogen associated molecular patterns modulate galectin expression in cow blood

  • 투고 : 2019.01.23
  • 심사 : 2019.09.05
  • 발행 : 2019.09.30
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초록

Pathogen-associated Molecular Patterns (PAMPs) are highly conserved structural motifs that are recognized by Pathogen Recognition receptors (PRRs) to initiate immune responses. Infection by these pathogens and the immune response to PAMPS such as lipopolysaccharide (LPS), Peptidoglycan (PGN), bacterial oligodeoxynucleotides [CpG oligodeoxynucleotides 2006 (CpG ODN2006) and CpG oligodeoxynucleotides 2216 (CpG ODN2216)], and viral RNA Polyinosinic-Polycytidylic Acid (Poly I:C), are associated with infectious and metabolic diseases in animals impacting health and production. It is established that PAMPs mediate the production of cytokines by binding to PRRs such as Toll-like receptors (TLR) on immune cells. Galectins (Gal) are carbohydrate-binding proteins that when expressed play essential roles in the resolution of infectious and metabolic diseases. Thus it is important to determine if the expression of galectin gene (LGALS) and Gal secretion in blood are affected by exposure to LPS and PGN, PolyI:C and bacterial CpG ODNs. LPS increased transcription of LGALS4 and 12 (2.5 and 2.02 folds respectively) and decreased secretion of Gal 4 (p < 0.05). PGN increased transcription of LGALS-1, -2, -3, -4, -7, and -12 (3.0, 2.3, 2.0, 4.1, 3.3, and 2.4 folds respectively) and secretion of Gal-8 and Gal-9 (p < 0.05). Poly I:C tended to increase the transcription of LGALS1, LGALS4, and LGALS8 (1.78, 1.88, and 1.73 folds respectively). Secretion of Gal-1, -3, -8 and nine were significantly increased in treated samples compared to control (p < 0.05). CpG ODN2006 did not cause any significant fold changes in LGALS transcription (FC < 2) but increased secretion of Gal-1, and-3 (p < 0.05) in plasma compared to control. Gal-4 was however reduced in plasma (p < 0.05). CpG ODN2216 increased transcription of LGALS1 and LGALS3 (3.8 and 1.6 folds respectively), but reduced LGALS2, LGALS4, LGALS7, and LGALS12 (-1.9, -2.0, -2.0 and; -2.7 folds respectively). Secretion of Gal-2 and -3 in plasma was increased compared to control (p < 0.05). Gal-4 secretion was reduced in plasma (p < 0.05). The results demonstrate that PAMPs differentially modulate galectin transcription and translation of galectins in cow blood.

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참고문헌

  1. LeBlanc SJ, Lissemore KD, Kelton DF, Duffield TF, Leslie KE. Major advances in disease prevention in dairy cattle. J Dairy Sci. 2006;89:1267-79. https://doi.org/10.3168/jds.S0022-0302(06)72195-6
  2. Bannerman DD. Pathogen-dependent induction of cytokines and other soluble inflammatory mediators during intramammary infection of dairy cows. J Anim Sci. 2009;87 Suppl 13:10-25. https://doi.org/10.2527/jas.2008-1187
  3. Sarukhan A, Martinez-Florensa M, Escoda-Ferran C, Carrasco E, Carreras E, Lozano F. Pattern recognition by CD6: a scavenger-like lymphocyte receptor. Curr Drug Targets 2016;17:640-50. https://doi.org/10.2174/1389450116666150316224308
  4. Schaefer TM, Fahey JV, Wright JA, Wira CR. Innate immunity in the human female reproductive tract: antiviral response of uterine epithelial cells to the TLR3 agonist poly (I:C). J Immunol. 2005;174:992-1002. https://doi.org/10.4049/jimmunol.174.2.992
  5. Nichani AK, Mena A, Popowych Y, Dent D, Townsend HG, Mutwiri GK, et al. In vivo immunostimulatory effects of CpG oligodeoxynucleotide in cattle and sheep. Vet Immunol Immunopathol. 2004;98:17-29. https://doi.org/10.1016/j.vetimm.2003.10.001
  6. Griebel PJ, Brownlie R, Manuja A, Nichani A, Mookherjee N, Popowych Y, et al. Bovine toll-like receptor 9: a comparative analysis of molecular structure, function and expression. Vet Immunol Immunopathol. 2005;108:11-6. https://doi.org/10.1016/j.vetimm.2005.07.012
  7. Asiamah EK, Adjei-Fremah S, Osei B, Ekwemalor K, Worku M. An extract of Sericea Lespedeza modulates production of inflammatory markers in pathogen associated molecular pattern (PAMP) activated ruminant blood. J Agr Sci. 2016;8:1.
  8. Asiamah EK, Adjei-Fremah S, Ekwemalor K, Worku M. Nystatin modulates genes in immunity and wingless signaling pathways in cow blood. J Mol Biol Res. 2017;7:1. https://doi.org/10.5539/jmbr.v7n1p1
  9. Adjei-Fremah S, Ekwemalor K, Asiamah E, Ismail H, Worku M. Transcriptional profiling of the effect of lipopolysaccharide (LPS) pretreatment in blood from probiotics-treated dairy cows. Genom Data 2016;10:15-8. https://doi.org/10.1016/j.gdata.2016.08.016
  10. Werling D, Jungi TW. TOLL-like receptors linking innate and adaptive immune response. Vet Immunol Immunopathol. 2003;91:1-12. https://doi.org/10.1016/S0165-2427(02)00228-3
  11. Plaizier JC, Krause DO, Gozho GN, McBride BW. Subacute ruminal acidosis in dairy cows: the physiological causes, incidence and consequences. Vet J. 2008;176:21-31. https://doi.org/10.1016/j.tvjl.2007.12.016
  12. Tilman D, Cassman KG, Matson PA, Naylor R, Polasky S. Agricultural sustainability and intensive production practices. Nature 2002;418:671-7. https://doi.org/10.1038/nature01014
  13. Rabinovich GA, Baum LG, Tinari N, Paganelli R, Natoli C, Liu FT, et al. Galectins and their ligands: amplifiers, silencers or tuners of the inflammatory response? Trends Immunol. 2002;23:313-20. https://doi.org/10.1016/S1471-4906(02)02232-9
  14. van Kooyk Y, Rabinovich GA. Protein-glycan interactions in the control of innate and adaptive immune responses. Nat Immunol. 2008;9:593-601. https://doi.org/10.1038/ni.f.203
  15. Vasta GR. Galectins as pattern recognition receptors: structure, function, and evolution. Adv Exp Med Biol. 2012;946:21-36. https://doi.org/10.1007/978-1-4614-0106-3_2
  16. Sato S, St-Pierre C, Bhaumik P, Nieminen J. Galectins in innate immunity: dual functions of host soluble ${\beta}$-galactoside-binding lectins as damage-associated molecular patterns (DAMPs) and as receptors for pathogen-associated molecular patterns (PAMPs). Immunol Rev. 2009;230:172-187. https://doi.org/10.1111/j.1600-065X.2009.00790.x
  17. Wang M, Wang L, Huang M, Yi Q, Guo Y, Gai Y, et al. A galectin from Eriocheir sinensis functions as pattern recognition receptor enhancing microbe agglutination and haemocytes encapsulation. Fish Shellfish Immunol. 2016;55:10-20. https://doi.org/10.1016/j.fsi.2016.04.019
  18. Mishra BB, Li Q, Steichen AL, Binstock BJ, Metzger DW, Teale JM, et al. Galectin-3 functions as an alarmin: pathogenic role for sepsis development in murine respiratory tularemia. PloS one 2013;8:e59616. https://doi.org/10.1371/journal.pone.0059616
  19. LeBlanc SJ, Duffield TF, Leslie KE, Bateman KG, Keefe GP, Walton JS, et al. Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. J Dairy Sci. 2002;85:2223-6. https://doi.org/10.3168/jds.S0022-0302(02)74302-6
  20. Oliver SP, Murinda SE, Jayarao BM. Impact of antibiotic use in adult dairy cows on antimicrobial resistance of veterinary and human pathogens: a comprehensive review. Foodborne pathog Dis. 2011;8:337-55. https://doi.org/10.1089/fpd.2010.0730
  21. Asiamah EK. Ex vivo effects of water extracts of sericea lespedeza on cow, sheep and goat blood [Ph.D. dissertation]. Greensboro, NC: North Carolina Agricultural and Technical State University; 2015.
  22. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2- ${\Delta}{\Delta}CT$ method. methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  23. Asiamah EK, Adjei-Fremah S, Ekwemalor K, Sordillo L, Worku M. Parity and periparturient period affects galectin gene expression in Holstein cow blood. J Appl Biotechn. 2018;6:20.
  24. Vasta GR. Roles of galectins in infection. Nat Rev Microbiol. 2009;7:424-38. https://doi.org/10.1038/nrmicro2146
  25. Kawai T, Akira S. The role of pattern-recognition receptors in innate immunity: update on toll-like receptors. Nat Immunol. 2010;11:373-84. https://doi.org/10.1038/ni.1863
  26. Adib-Conquy M, Cavaillon JM. Stress molecules in sepsis and systemic inflammatory response syndrome. FEBS Lett. 2007;581:3723-33. https://doi.org/10.1016/j.febslet.2007.03.074
  27. Ekwemalor K, Asiamah E, Osei B, Ismail H, Worku M. Evaluation of the effect of probiotic administration on gene expression in goat blood. J Mol Biol Res. 2017;7:88. https://doi.org/10.5539/jmbr.v7n1p88
  28. Huflejt ME, Leffler H. Galectin-4 in normal tissues and cancer. Glycoconjugate J. 2003;20:247-55. https://doi.org/10.1023/B:GLYC.0000025819.54723.a0
  29. Paclik D, Danese S, Berndt U, Wiedenmann B, Dignass A, Sturm A. Galectin-4 controls intestinal inflammation by selective regulation of peripheral and mucosal T cell apoptosis and cell cycle. PLoS One 2008;3:e2629. https://doi.org/10.1371/journal.pone.0002629
  30. Yang RY, Havel PJ, Liu FT. Galectin-12: a protein associated with lipid droplets that regulates lipid metabolism and energy balance. Adipocyte 2012;1:96-100. https://doi.org/10.4161/adip.19465
  31. Gonzalez MI, Rubinstein N, Ilarregui JM, Toscano MA, Sanjuan NA, Rabinovich GA. Regulated expression of galectin-1 after in vitro productive infection with herpes simplex virus type I: implications for T cell apoptosis. Int J Immunopathol Pharmacol. 2005;18:615-23. https://doi.org/10.1177/039463200501800402
  32. Rabinovich GA, Toscano MA, Jackson SS, Vasta GR. Functions of cell surface galectin-glycoprotein lattices. Curr Opin Struct Biol. 2007;17:513-20. https://doi.org/10.1016/j.sbi.2007.09.002
  33. Garner OB, Aguilar HC, Fulcher JA, Levroney EL, Harrison R, Wright L, et al. Endothelial galectin-1 binds to specific glycans on nipah virus fusion protein and inhibits maturation, mobility, and function to block syncytia formation. PLoS pathog. 2010;6:e1000993. https://doi.org/10.1371/journal.ppat.1000993
  34. Zhou Y, Guo M, Wang X, Li J, Wang Y, Ye L, et al. TLR3 activation efficiency by high or low molecular mass poly I: C. Innate Immun. 2013;19:184-92. https://doi.org/10.1177/1753425912459975
  35. Miettinen J. Activation of innate immune response in human macrophages by Herpes simplex virus-1 and crystallized monosodium urate [Ph.D. dissertation]. Helsinki, Finland: University of Helsinki; 2014.
  36. Thurston TL, Wandel MP, von Muhlinen N, Foeglein A, Randow F. Galectin-8 targets damaged vesicles for autophagy to defend cells against bacterial invasion. Nature 2012;482:414-8. https://doi.org/10.1038/nature10744
  37. Wu J, Chen ZJ. Innate immune sensing and signaling of cytosolic nucleic acids. Ann Rev Immunol. 2014;32:461-88. https://doi.org/10.1146/annurev-immunol-032713-120156
  38. Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, et al. Erratum a toll-like receptor recognizes bacterial DNA. Nature 2001;409:646. https://doi.org/10.1038/35054604
  39. Arthur JS, Ley SC. Mitogen-activated protein kinases in innate immunity. Nat Rev Immunol. 2013;13:679-92. https://doi.org/10.1038/nri3495
  40. Krug A, Rothenfusser S, Hornung V, Jahrsdorfer B, Blackwell S, Ballas ZK, et al. Identification of CpG oligonucleotide sequences with high induction of IFN-${\alpha}/{\beta}$ in plasmacytoid dendritic cells. Eur J Immunol. 2001;31:2154-63. https://doi.org/10.1002/1521-4141(200107)31:7<2154::AID-IMMU2154>3.0.CO;2-U
  41. Rothenfusser S, Hornung V, Krug A, Towarowski A, Krieg AM, Endres S, et al. Distinct CpG oligonucleotide sequences activate human ${\gamma}\;{\delta}$ T cells via interferon-${\alpha}/-{\beta}$. Eur J Immunol. 2001;31:3525-34. https://doi.org/10.1002/1521-4141(200112)31:12<3525::AID-IMMU3525>3.0.CO;2-5
  42. Worku M, Morris A. Binding of different forms of lipopolysaccharide and gene expression in bovine blood neutrophils. J Dairy Sci. 2009;92:3185-93. https://doi.org/10.3168/jds.2008-1905

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