Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Antioxidant, anti-cholinesterase, and inflammation inhibitory activities of fruiting bodies of Phallus impudicus var. impudicus L.

말뚝버섯 자실체의 항산화, 항콜린에스테라제 및 염증 저해 활성

  • Yoon, Ki Nam (Department of Clinical Laboratory Science, Ansan University) ;
  • Lee, Tae Soo (Division of Life Sciences, Incheon National University)
  • Received : 2019.08.30
  • Accepted : 2019.09.23
  • Published : 2019.09.30
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Abstract

Phallus impudicus var. impudicus L. is an edible mushroom that has long been used as folk medicine in China. The aim of this study was to evaluate the antioxidant, anti-cholinesterase, and inflammation inhibitory activities of a methanol extract of fruiting bodies of P. impudicus var. impudicus L. The extract exhibited good 1,1-diphenyl-2-picrylhydrazyl scavenging activity, excellent ferrous ion chelating activity, and moderate hydroxyl radical scavenging activity compared with BHT at 2.0 mg/ml. However, the reducing power of the extract was significantly lower than that the BHT positive control. Although the inhibitory activities of methanol extract on acetylcholinesterase and butyryl cholinesterase were significantly lower than the galanthamine positive control at the concentration tested, the inhibition of acetylcholinesterase and butyryl cholinesterase was 52.83% and 55.17%, respectively, at 1.0 mg/ml. The methanol extract also demonstrated excellent inhibition of inflammation-related activities, such as production of nitric oxide in lipopolysaccharide-induced RAW 264.7 macrophage cells and acute edema induced by administration of carrageenan on the hind paw of rats. The collective results suggest that the fruiting body of P. impudicus var. impudicus L. might be a good source of antioxidant, anti-cholinesterase, and anti-inflammation compounds.

본 연구에서는 말뚝버섯의 자실체 메탄올 추출물의 항산화, cholinesterase 저해 및 항염증 효과를 탐색하였다. DPPH 라디칼 소거능, hydroxyl radical 소거능, 철 이온 제거능 및 환원력 등의 항산화 효과를 측정한 결과 DPPH 라디칼 소거능, hydroxyl radical 소거능 및 환원력은 양성대조군으로 사용한 BHT에 비해 낮았으나 실험에 시용한 2.0 mg/ml의 농도에서 50% 이상의 저해효과를 나타내었고 철 이온 제거능은 BHT에 비해 높게 나타나서 다른 종류의 식의약용 버섯에 비해 항산화 효과가 우수하였다. 치매환자의 기억력 감퇴와 관련된 acetylcholinesterase와 butyrylcholinesterase의 저해실험에서 말뚝버섯 자실체의 메탄올 추출물은 실험에 사용한 전 농도 범위에서 양성대조군인 galanthamine에 비해 유의하게 낮았지만 1.0 mg/ml의 농도에서 50% 이상의 저해 효과를 나타냈다. In vitro 항염증 실험에서 RAW 264.7 대식세포에 서로 다른 농도의 메탄올 추출물을 처리한 후 염증 유발물질인 LPS를 처리하여 RAW 264.7 세포가 생성한 NO의 양을 측정한 결과 추출물을 투여한 실험군의 NO 농도가 LPS만 단독으로 처리한 양성대조군에 비해 유의하게 낮았고 처리한 메탄올 추출물의 농도가 증가함에 따라 생성된 NO의 양은 유의하게 감소하는 경향을 나타났다. 또한 in vivo 항염증 실험에서 먼저 각기 다른 농도의 메탄올 추출물을 생쥐의 뒷발에 주사한 후 추가로 기염제인 carrageenan을 주사하여 흰쥐 뒷발에 유도된 부종 (edema)이 추출물에 의해 저해되는 정도와 염증 치료제로 처방되는 indomethacin을 양성대조군으로 하여 실험을 진행하였다. 실험 결과 흰쥐에 주사한 말뚝버섯 자실체의 메탄올 추출물의 농도가 증가함에 따라 흰쥐 뒷발에 유도된 부종의 용적도 농도 의존적으로 유의하게 감소하는 것이 관찰되어 말뚝버섯 자실체에는 염증을 저해하는 성분이 함유되어 있는 것으로 사료되었다. 따라서 말뚝버섯 자실체의 메탄올 추출물에는 항산화, acetylcholinesterase과 butyrylcholinesterase의 저해 및 항염증 효과를 나타내는 유용 성분이 함유되어 있어 앞으로 의약용 기초 소재로서의 이용 연구가 필요하다고 사료된다.

Keywords

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