생태와환경 (Korean Journal of Ecology and Environment)

  • 제52권4호
  • /
  • Pages.385-393
  • /
  • 2019
  • /
  • 2288-1115(pISSN)
  • /
  • 2288-1123(eISSN)
한국수생태학회 (The Korean Society of Limnology)
권호 표지
DOI QR코드

DOI QR Code

Classification and Distribution of Chironomidae (Diptera) using DNA Barcoding at Urban Streams in Gwangju, South Korea

  • 투고 : 2019.10.15
  • 심사 : 2019.12.11
  • 발행 : 2019.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Chironomid communities are indicators of water pollution because of their ability to thrive under freshwater conditions. However, it is difficult to distinguish between chironomid larvae based on morphology. DNA barcoding, based on nucleotide sequences of marker genes, can be used to identify chironomid larvae. Samples of chironomid larvae were collected from Gwangju Stream and Pungyeongjeong Stream, tributaries of the Yeongsan River in South Korea. We identified 3 subfamilies, 13 genera, 16 species, and 1 cryptic species. There were 7 genera and 10 species from the subfamily Chironominae, 5 genera and 5 species from subfamily Orthocladiinae, 1 genus and 1 species from subfamily Tanipodinae, and the cryptic chironomid species of the family Chironomidae. There were 21 individuals from, 7 species and 1 cryptic species from the Gwangju Stream and 24 individuals, belonging to 10 species from the Pungyeongjeong Stream. The only species detected in both streams was Cricotopus bicinctus. The relationship between water quality and the species detected was difficult to explain, but the number of species showed a tendency to increase at sites where water quality was poor. Additional investigations and studies are needed to understand the relationship between water quality and the chironomid species occurring in these two streams.

키워드

참고문헌

  1. Aagaard, K., J.O. Solem, T. Bongard and O. Hanssen. 2004. Studies of aquatic insects in the Atna River 1987-2002. Hydrobiologia 521: 87-105. https://doi.org/10.1023/B:HYDR.0000026352.40631.37
  2. Armitage, P.D. 1995. Chironomidae as food, p. 425-428. In: The Chironomidae: the biology and ecology of non-biting midge (Armitage, P., P.S. Cranston and L.C.V. Pinder, eds.). Chapman and Hall, London, UK.
  3. Carew, M.E., V. Pettigrove and A.A. Hoffmann. 2003. Identifying Chironomids (Diptera: Chironomidae) for biological monitoring with PCR-RFLP. Bulletin of Entomological Research 93: 483-490. https://doi.org/10.1079/BER2003268
  4. Di Veroli, A., F. Santoro, M. Pallottini, R. Selvaggi, F. Scardazza, D. Cappelletti and E. Goretti. 2014. Deformities of chironomid larvae and heavy metal pollution: From laboratory to field studies. Chemosphere 112: 9-17. https://doi.org/10.1016/j.chemosphere.2014.03.053
  5. Ekrem, T. and E. Willassen. 2004. Exploring Tanytarsini relationships (Diptera: Chrionomidae) using mitochondrial COII gene sequences. Insect Systematics and Evolution 35: 263-276. https://doi.org/10.1163/187631204788920248
  6. Ekrem, T., E. Willassen and E. Stru. 2007. A comprehensive DNA sequence library is essential for identification with DNA barcodes. Molecular Phylogenetics and Evolution 43: 530-542. https://doi.org/10.1016/j.ympev.2006.11.021
  7. Folmer, O., M. Black, W. Hoeh, R. Lutz and R. Vrijenjoek. 1994. DNA primers for amplication of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294-299.
  8. Hebert, P.D.M., S. Ratnasingham, E.V. Zakharov, A.C. Telfer, V. Levesque-Beaudin, M.A. Milton, S. Pedersen, P. Jannetta and J.R. deWaard. 2016. Counting animal species with DNA barcodes: Canadian insects. Philosophical Transactions of the Royal Society B 371(1702): 20150333. https://doi.org/10.1098/rstb.2015.0333
  9. Kim, J.Y., J.H. Lee and H.I. Ree. 2001. Seasonal population dynamics of chironomids midges (Diptera: Chironomidae) emerging from reclaimed rice fields in Seosan, Korea in 1997-1999. Korean Journal of the Entomology 31(4): 225-232.
  10. Kim, B.S., Y.K. Park, S.S. Hong, Y.J. Yang, K.H. Park, M.H. Jeong, S.R. Kim, K.H. Park, W.H. Yeh, D.H. Kim, M.K. Hong, Y.J. Ahn and J.S. Shin. 2009. Comparison of acute toxicity of molinate on two aquatic insects, Chironomus riparius and Cloeon dipterum in different larval stages. The Korean Journal of Pesticide Science 13(4): 256-261.
  11. Kim, S.M., K.H. Song, H.I. Ree and W. Kim. 2012. A DNA barcode library for Korean Chironomidae (Insecta: Diptera) and indexes for defining barcode gap. Molecules and Cells 33: 9-17. https://doi.org/10.1007/s10059-012-2151-2
  12. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111-120. https://doi.org/10.1007/BF01731581
  13. Kuncham, R., T. Thayumanavan and G.V.S. Reddy. 2016. Phylogenetic relationship of some Indian chironomids based on mitochondrial DNA cytochrome oxidase I. Advances in Bioresearch 7(4): 46-51.
  14. Lindergaard, C. 1995. Classification of water-bodies and pollution, p. 385-404. In: The Chironomidae: the biology and ecology of non-biting midge (Armitage, P., P.S. Cranston, and L.C.V. Pinder, eds.). Chapman and Hall, London, UK.
  15. Makarevich, I.F., E.V. Berezikov, V.P. Guryev and A.G. Blinov. 2000. Molecular phylogeny of the Chironomus genus deduced from nucleotide sequences of two muclear genes, ssp160 and the globin 2b gene. Molecular Biology 34: 606-612. https://doi.org/10.1007/BF02759569
  16. Matt, N.K., L.M. Bryant and S. Vink. 2017. Differential gene expression of Australian Cricotopus draysoni (Diptera: Chironomiae) populations reveals seasonal association in detoxification gene regulation. Nature 7: 14263.
  17. Matsuhashi, T., R. Matsuda, T. Mano and M.C. Yoshida. 1999. Microevolution of the mitochondrial DNA control region in the Hapanese brown bear (Ursus arctos) population. Molecular Biology and Evolution Society 16: 676-684. https://doi.org/10.1093/oxfordjournals.molbev.a026150
  18. MOE (Ministry of Environment). 2015. The standard method on water pollution, National Institute of Environmental Research. Incheon, Korea.
  19. MOE (Ministry of Environment). 2016. Biomonitoring survey and assessment manual, National Institute of Environmental Research. Incheon, Korea.
  20. Pfenninger, M., C. Nowak, C. Kley, D. Steinke and B. Streit. 2007. Utility of DNA taxonomy and barcoding for the inference of larval community structure in morphologically cryptic Chironomus (Diptera) species. Molecular Ecology 16: 1957-1968. https://doi.org/10.1111/j.1365-294X.2006.03136.x
  21. Sharley, D.J., V. Pettigrove and Y.M. Parson. 2004. Molecular Identification of Chironomus spp. (Diptera) for biomonitoring of aquatic ecosystems. Australian Journal of Entomology 43(4): 359-365. https://doi.org/10.1111/j.1440-6055.2004.00417.x
  22. Surber, E.W., 2011. Cricotopus bicinctus, a midgefly resistant to electroplating wastes. Transactions of the American Fisheries Society 88: 111-116. https://doi.org/10.1577/1548-8659(1959)88[111:cbamrt]2.0.co;2
  23. Verneaux, V. and J. Verneaux. 2002. Assessing lake functioning using the macrobenthic community with special reference to Chironomidae (Diptera). A subalpine lake (Lake Annecy) as an example. Archiv fuer Hydrobiologie 154: 61-78. https://doi.org/10.1127/archiv-hydrobiol/154/2002/61
  24. Wright, J.F. 1984. The chironomid larvae of a small chalk stream in Berkshire, England. Ecological Entomology 9: 231-238. https://doi.org/10.1111/j.1365-2311.1984.tb00718.x
  25. Yoon, T.J., M.J. Baek, J.M. Hwang, H.J. Kang, W.Y. Choi, J.M. Hur and Y.J. Bae. 2011. Analysis of molecular operational taxonomic unit (MOTU) in Chironomids (Chironomidae, Diptera) for use in indicator organisms for water quality assessment. Korean Journal of Nature Conservation 5(2): 77-82.
  26. Zhang, D.X. and G.M. Hewitt. 1997. Assessment of the universality and utility of a set of conserved mitochondrial primers in insects. Insect Molecular Biology 6: 143-150. https://doi.org/10.1111/j.1365-2583.1997.tb00082.x