Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Analysis of the Concentration of Metal Elements in Freshwater Fish Otolith Using LA-ICP/MS

LA-ICP/MS를 이용한 담수 어류 이석 내 금속 원소 농도 분석

  • Park, Hyun Woo (Environmental Measurement and Analysis Center, National Institute of Environmental Research) ;
  • Yoon, Suk-Hee (Environmental Measurement and Analysis Center, National Institute of Environmental Research) ;
  • Park, Jaeseon (Environmental Measurement and Analysis Center, National Institute of Environmental Research) ;
  • Lim, Bo-Ra (Environmental Measurement and Analysis Center, National Institute of Environmental Research) ;
  • Lee, Hyeri (Environmental Measurement and Analysis Center, National Institute of Environmental Research) ;
  • Choi, Jong Woo (Environmental Measurement and Analysis Center, National Institute of Environmental Research)
  • 박현우 (국립환경과학원 환경측정분석센터) ;
  • 윤숙희 (국립환경과학원 환경측정분석센터) ;
  • 박재선 (국립환경과학원 환경측정분석센터) ;
  • 임보라 (국립환경과학원 환경측정분석센터) ;
  • 이혜리 (국립환경과학원 환경측정분석센터) ;
  • 최종우 (국립환경과학원 환경측정분석센터)
  • Received : 2019.11.04
  • Accepted : 2019.11.25
  • Published : 2019.12.31
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Abstract

In this study, the possibility of a follow-up study on environmental pollution in domestic freshwater was identified by analyzing fish otoliths using Laser ablation-inductively coupled mass spectrometry (LA-ICP/MS). Fish otolith are known to be affected by the environment in which fish live. As a result, research on this subject is active in many countries; however, this is not the case in Korea. Therefore, in this study, the possibility of tracing environmental pollution using fish otoliths was identified by analyzing the components of metal elements used as indicators for environmental pollution. For the component analysis of metallic elements LA-ICP/MS, which can shorten analysis time by reducing the pretreatment process, was used. Sampling was conducted by dividing the research and the background area and carp, a freshwater species, was selected as the experimental fish species subject. Based on the established LA-ICP/MS conditions, the concentration of the metallic elements in the fish otoliths collected in the research area was 2202.9 mg kg-1, 2.03 times higher than the 1,086.3 mg kg-1 in the background area. All elements except for Li and U, were found to be higher in the research area than in the background area. Compared with the sediment measuring net analysis data, the distribution tendency of Zn, Pb, and Cu in sediment metal element concentrations in the two regions and distribution of metal element concentration in fish otoliths were similarly shown. These results confirm that fish otoliths can be used to track environmental pollutants, such as in sediments.

본 연구는 Laser ablation ICP/MS (이하 LA-ICP/MS)를 이용한 환경오염 추적연구를 위하여 어류 내 이석을 분석하였다. 어류의 이석은 어류가 서식하는 환경에 영향을 받는 것으로 알려져 있어 국외에서는 이를 활용한 연구가 활발하나 국내에서는 이에 대한 연구가 미비한 실정이다. 그래서 본 연구에서는 환경오염의 지표로 사용되는 금속 원소의 성분 분석을 통하여 어류 이석을 이용한 환경오염 추적 가능성을 파악하고자 하였다. 또한 금속 원소의 성분 분석을 위해서는 전처리 과정을 줄여 분석 시간을 단축시킬 수 있는 것으로 알려진 LA-ICP/MS를 이용하였다. 시료채취는 연구지역과 배경지역으로 나누어 실험을 진행하였고, 실험 어종은 담수종인 잉어를 선정하였다. LA-ICP의 분석 최적 조건을 정립하기 위하여 이석 표준물질인 FEBS-1을 이용하여 9개 금속 원소(Li, Mg, Mn, Cu, Zn, Sr, Ba, Pb, U)의 정확도와 정밀도를 확인하였다. 정립한 조건을 이용하여 실제시료를 분석한 결과, 연구지역에서 채집한 어류 이석 내 금속 원소 성분의 총 농도가 2202.9 mg kg-1으로 배경 지역의 1086.3 mg kg-1보다 2.03배 높게 측정되었다. 원소별로는 Li과 U을 제외한 모든 원소가 연구 지역이 배경지역보다 높게 나타났다. 그리고 퇴적물 측정망 분석 자료와 비교한 결과, Zn, Pb, Cu가 두 지역의 퇴적물 금속 원소 농도 분포와 어류 이석 내 금속 원소 농도 분포 경향이 유사하게 나타났다. 이러한 결과로 보아 어류 내 이석은 퇴적물과 같이 환경오염원을 추적하는 데 활용할 수 있다는 것을 확인하였다.

Keywords

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