Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Detection of Human Anti-Trypanosoma cruzi Antibody with Recombinant Fragmented Ribosomal P Protein

  • Kim, Yeong Hoon (Department of Ophthalmology, College of Medicine, The Catholic University of Korea) ;
  • Yang, Zhaoshou (Department of Parasitology, College of Medicine, The Catholic University of Korea) ;
  • Lee, Jihoo (GenBody Inc.) ;
  • Ahn, Hye-Jin (Department of Parasitology, College of Medicine, The Catholic University of Korea) ;
  • Chong, Chom-Kyu (GenBody Inc.) ;
  • Maricondi, Wagner (WAMA Diagnostica, Aldo Germano Clein) ;
  • Dias, Ronaldo F. (WAMA Diagnostica, Aldo Germano Clein) ;
  • Nam, Ho-Woo (Department of Parasitology, College of Medicine, The Catholic University of Korea)
  • Received : 2019.04.15
  • Accepted : 2019.07.15
  • Published : 2019.08.31
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Abstract

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and is endemic in many Latin American countries. Diagnosis is based on serologic testing and the WHO recommends two or more serological tests for confirmation. Acidic ribosomal P protein of T. cruzi showed strong reactivity against positive sera of patients, and we cloned the protein after fragmenting it to enhance its antigenicity and solubility. Twelve positive sera of Chagas disease patients were reacted with the fragmented ribosomal P protein using western blot. Detection rate and density for each fragment were determined. Fragments F1R1, F1R2, and F2R1 showed 100% rate of detection, and average density scoring of 2.00, 1.67, and 2.42 from a maximum of 3.0, respectively. Therefore, the F2R1 fragment of the ribosomal P protein of T. cruzi could be a promising antigen to use in the diagnosis of Chagas disease in endemic regions with high specificity and sensitivity.

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References

  1. Rassi A Jr, Rassi A, Marin-Neto JA. Chagas disease. Lancet 2010; 375: 1388-1402. https://doi.org/10.1016/S0140-6736(10)60061-X
  2. Hotez PJ, Bottazzi ME, Franco-Paredes C, Ault SK, Periago MR. The neglected tropical diseases of Latin America and the Caribbean: a review of disease burden and distribution and a roadmap for control and elimination. PLoS Negl Trop Dis 2008; 2: e300. https://doi.org/10.1371/journal.pntd.0000300
  3. Mathers CD, Ezzati M, Lopez AD. Measuring the burden of neglected tropical diseases: the global burden of disease framework. PLoS Negl Trop Dis 2007; 1: e114. https://doi.org/10.1371/journal.pntd.0000114
  4. Prata A. Clinical and epidemiological aspects of Chagas disease. Lancet Infect Dis 2001; 1: 92-100. https://doi.org/10.1016/S1473-3099(01)00065-2
  5. World Health Organization. Chagas disease (American trypanosomiasis) [Internet]; [cited 2019 Mar 20]. Available from: https://www.who.int/news-room/fact-sheets/detail/chagas-disease-(american-trypanosomiasis).
  6. World Health Organization. Second WHO consultation on the development of a WHO reference panel for the control of Chagas diagnostic tests [Internet]; [cited 2019 Mar 20]. Available from: https://www.who.int/bloodproducts/ref_materials/chagas_dev_ref_materials/en/
  7. Frasch AC, Cazzulo JJ, Aslund L, Pettersson U. Comparison of genes encoding Trypanosoma cruzi antigens. Parasitol Today 1991; 7: 148-151. https://doi.org/10.1016/0169-4758(91)90284-U
  8. Houghton RL, Benson DR, Reynolds LD, McNeill PD, Sleath PR, Lodes MJ, Skeiky YA, Leiby DA, Badaro R, Reed SG. A multi-epitope synthetic peptide and recombinant protein for the detection of antibodies to Trypanosoma cruzi in radioimmunoprecipitation-confirmed and consensus-positive sera. J Infect Dis 1999; 179: 1226-1234. https://doi.org/10.1086/314723
  9. Gomez Barroso JA, Aguilar CF. Chagas disease: a homology model for the three-dimensional structure of the Trypanosoma cruzi ribosomal P0 antigenic protein. Eur Biophys J 2014; 43: 361-366. https://doi.org/10.1007/s00249-014-0967-8
  10. Bocharov EV, Sobol AG, Pavlov KV, Korzhnev DM, Jaravine VA, Gudkov AT, Arseniev AS. From structure and dynamics of protein L7/L12 to molecular switching in ribosome. J Biol Chem 2004; 279: 17697-17706. https://doi.org/10.1074/jbc.M313384200