한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제34권4호
  • /
  • Pages.361-366
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  • 2019
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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다슬기(Semisulcospira libertine)의 미생물 오염도 평가 및 해감 제거공정에 따른 저감화 효과

Investigation of Microbial Contamination in Semisulcospira libertine and Evaluation of Its Reduction Effects by Sediment Removal Treatment

  • 최만석 (경상대학교 해양산업연구소) ;
  • 전은비 (경상대학교 해양산업연구소) ;
  • 최승호 (한국쓰리엠 주식회사) ;
  • 방현조 (한국쓰리엠 주식회사) ;
  • 박신영 (경상대학교 해양산업연구소)
  • 투고 : 2019.04.29
  • 심사 : 2019.06.13
  • 발행 : 2019.08.30
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초록

본 연구에서는 다슬기 중의 자연균총의 정량적 오염도 분석과 해감 제거 공정(지하수, 상수)에 따른 저감화 효과를 조사하였다. 다슬기 중의 일반세균, 대장균군과 대장균, 진균 및 황색포도상구균의 정량적 검출을 위해 표준평판법을 사용하여 각각 plate count agar (PCA), potato dextrose agar (PDA), 대장균군/대장균용 3M Petrifilm 및 황색포도상구균용 3M Petrifilm에 도말하여 집락을 계수하였다. 지하수와 상수 처리에 따른 이들 균의 저감화 정도 역시 동일한 방법으로 수행되었다. 다슬기 원료 중의 미생물 오염도 분석시, 일반세균, 대장균군 및 진균은 각각 6.40, 2.70 및 $6.79{\log}_{10}CFU/g$로 조사되었다. 대장균과 황색포도상구균은 검출되지 않았다 (검출 한계: < $1{\log}_{10}CFU/g$). 그러나, 지하수로 해감 제거한 다슬기의 경우, 분석된 모든 미생물의 정량적 오염도가 원료 다슬기에 비해 높게 검출되었다. 특히 원료 다슬기에서 불검출이었던 대장균도 $2.46{\log}_{10}CFU/g$ 검출되었다. 상수로 해감 제거한 다슬기의 일반세균은 유의적으로 저감화 되지 않았으며(p<0.05), 대장균군은 유의적으로 증가하는 경향을 나타내었다(p>0.05). 진균만이 약간의 저감화(0.2 log 이하)를 유의적으로 보였다(p>0.05). 본 연구결과 지하수와 상수를 이용해서 해감 제거하는 공정만으로는 다슬기의 미생물학적 안전성을 확보하기 곤란한 것으로 판단된다.

In this study, microbial contamivation semisulcospira libertine and effect of sedimentation treatment of major bacterial and fungal pathogens were investigated. The total aerobic bacteria, coliforms, Escherichia coli, Staphylococcus aureus, and yeast and mold present in raw and water-dipped Semisulcospira libertine were enumerated using the standard plate count methods on using the standard plate method on potato dextrose agar (PDA), 3M Petrifilm for coliforms / E. coli, 3M Petrifilm for S. aureus, and plate count agar (PCA), respectively. In analysis of microbial contamination of raw Semisulcospira libertine, the total aerobic bacteria, coliforms, and yeast and mold were monitored as 6.40, 2.70, and $6.79{\log}_{10}CFU/g$, respectively. Both E. coli and S. aureus were not detected (detection limit: 10 CFU/g). However, Semisulcospira libertine dipped in ground water for 3 hours had higher contamination levels of all natural indigenous microorganisms than raw Semisulcospira libertine. Especially, E. coli was detected as $2.46{\log}_{10}CFU/g$ in the ground water-dipped Semisulcospira libertine. The total aerobic bacteria in the ground water-dipped Semisulcospira libertine was not significantly reduced (p>0.05) compared to that in the raw Semisulcospira libertine. Moreover, coliforms were significantly increased (p>0.05) in all water-dipped Semisulcospira libertine. Only fungi were slightly reduced (less than 0.2 log) (p>0.05) in the tap water-dipped Semisulcospira libertine by comparison with the raw Semisulcospira libertine. The results of this study suggest that the use of chemical sterilizing agents and other physical methods in the washing stage will be necessary for the microbial reduction in raw Semisulcospira libertine because the use of sediment removal treatment by ground or tap water did not affect the microbiological safety of the raw Semisulcospira libertine.

키워드

참고문헌

  1. Kim, Y.H., Lee, T.K., Cha, Y.S.: Studies of the nutritive component of black snail (Semisulcospiria libertina). J. Agric. Life Sci., 16, 101-105 (1985).
  2. Shim, T.H., Han, K.S., Lee, T.J., Cheong, E.H., Lee, H.K.: Composition of lipid and amino acid in Semisulcospiria lgorrschei tissue. J. Food Hyg. Saf., 9, 81-87 (1994).
  3. Kim, I.H.: An excellent means and principles of medical herbs. p. 29. In: Naturopathic medicine medical herbs. Kim, Y.S. (ed). Insandongcheon press., Hamyang, Korea (1999).
  4. Kown, O.K., Park, G.M., Lee, J.S.: Coloured shells of Korea. Academy Publishing Company, Seoul, Korea p. 445 (1993).
  5. Song, J.H, Kang, J.H.: The current status and tasks of Marsh snail restocking project based on economic performance evaluation. J. Fish. Mar. Sci. Educ., 28, 450-455 (2016). https://doi.org/10.13000/JFMSE.2016.28.2.450
  6. NIFS (National institute of fisheries science): Studies on Semisulcospira libertine culture technology. Final Report (2003).
  7. Mishra, R., Srdikar.: Depuration of meretrix casta. Indian. J. Anim. Sci., 59, 1360 (1990).
  8. Sangrungruang, K., Sahavacharin, S.: Deouration of some economically important bivalves in Thailand. Asean. Food J., 4, 101-106 (1990).
  9. Hong, S.P, Kim, D.S, Kim, Y.M.: Effect of water temperature, salt and $MgCl_2$ concentration on sand ejection characteristics of short Neck clam, Luditapes philippinarum, Korean J. Fish. Aquat. Sci., 30, 114-118 (1997).
  10. Kim, Y.K., Moon, H.S., Lee, M.H., Park, M.J., Lim, C.W., Park, H.Y., Park, J.I., Yoon, H.D., Kim, D.H.: Biological activities of seven Melania snails in Korea. Korean J. Fish. Aquat. Sci. 42, 434-441 (2009). https://doi.org/10.5657/kfas.2009.42.5.434
  11. Lim, C.W., Kim, Y.K., Kim, D.H., Park, J.I., Lee, M.H., Park, H.Y., Jang, M.S.: Comparison of quality characteristics of Melania snails in Korea. Korean J. Fish. Aquat. Sci. 42, 555-560 (2009). https://doi.org/10.5657/kfas.2009.42.6.555
  12. Moon, S.K., Kim, I.S., Lim, C.W., Yoon, N.Y., Jeong, B.Y.: Proximate and fatty acid compositions of commercial domestic and imported Melania Snails Semisulscospira sp. Korean J. Fish. Aquat. Sci., 48, 977-981 (2015). https://doi.org/10.5657/KFAS.2015.0977
  13. Lee, M.H., Kim, Y.K., Moon, H.S., Kim, Y.A., Yoon, N.Y., Lim, C.W., Park, H.Y., Kim, D.H.: Antioxidant activities of five Melania snails of the genus Semisulcospira in Korea. Korean J. Fish. Aquat. Sci., 43, 188-194 (2010). https://doi.org/10.5657/kfas.2010.43.3.188
  14. Pyo, S.E.: Anti-diabetic activation of enzymatic Semisulcospira coreama hydrolysates. MS thesis. Silla University Graduate School. Busan, Korea (2016).
  15. KFDA (Korean Food and Drug Administration): Comparatives studies on foreign countries' standards for the treatment of contaminants in food raw materials and efficient uses of raw materials. Final Report (2016).
  16. Solberg, M., Buckalew, J.J., Chen, C.C., Schaffner, D.W., O'Neil, K., Mcdowell, J., Post, L.S., Boderck, M.: Microbiological safety assurance system for foodservice facilities. Food Technol., 44, 68-73 (1990).
  17. Donnelly, C.W., Briggs, E.H.: Psychrotrophic growth and thermal inactivation of Listeria monocytogenes as function of milk composition. J. Food Prot., 49, 994-998 (1986). https://doi.org/10.4315/0362-028X-49.12.994
  18. Bibek, R. and Arun, B.: Fundamental food microbiology 4th ED. Bioscience Publishing Company, Gyunggi-do, pp. 17-28, 291-295 (2013).
  19. Lee, J.D, Jung, Y.K, Joo, W.H.: Mycological research. Sejong Book Publishing Company, Seoul, Korea pp. 107-144 (1994).
  20. Bruno, D.W., Wood, B.P.: Saprolegnia and other oomycetes. pp. 599-659. In: Fish Diseases and Disorders. Wood, P. T. K., Bruno, D. W. (eds). Wallingford., UK (1999).
  21. Song, K.C., Mok, J.S., Kang, C.S., Chang, D.S.: Sand elimination in shortnecked clam, Ruditapes philippinarum, harvested from western coast of Korea. Korean J. Fish. Aquat. Sci., 34, 179-183 (2001).
  22. Song, K.C., Mok, J.S., Kang, C.S., Chang, D.S.: Sand Elimination and Microbial Depuration in surf clam, Mactra veneriformis, Harvested from Western Coast of Korea. Korean J. Fish. Aquat. Sci., 34, 184-189 (2001).
  23. Kim, H.S.: Microbial hazard analysis of manufacturing processes for Takju. MS thesis. Chung-Ang University Graduate School. Seoul, Korea (2016).
  24. Kim, S.R, Oh, K.W, Lee, M.H., Jung, C.S., Lee, S.H., Park, S.J., Park, J.H., Ryu, K.W., Kim, B.S., Kim, D.H., Yun, J.C., Chung, D.H.: Effect of electrolyzed water combined with ultrasound and organic acids to reduce Salmonella Typhimurium, Staphylococcus aureus, Bacillus cereus on Perilla Leaves. J. Food Hyg. Saf., 27, 264-270 (2012). https://doi.org/10.13103/JFHS.2012.27.3.264
  25. Kim, C., Hung, Y.C., Brachett, R.E.: Efficacy of electrolyzed oxidizing(EO) and chemically modified water on different types of food-borne pathogens. Int. J. Food Microbiol., 61,199-207 (2000). https://doi.org/10.1016/S0168-1605(00)00405-0
  26. Zhou, B., Feng, H., Luo, Y.: Ultrasound enhanced sanitizer efficacy in reduction of Escherichia coli O157:H7 population on spinach leaves. J. Food Sci., 74, 308-313 (2009). https://doi.org/10.1111/j.1750-3841.2009.01247.x
  27. Choi, J.I., Kim, H.J., Kim, J.H., Ahn, D.H., Chun, B.S., Lee, J.W.: Application of gamma ray irradiation to the microbiological safety of dried seafood products. Korean J. Fish. Aquat. Sci., 43, 169-173 (2010). https://doi.org/10.5657/kfas.2010.43.2.169