Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification and Characterization of the Replication Region of Virulence Plasmid pEIB202 in Edwardsiella piscicida

  • Chang, Xinyue (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Teng, Chengli (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Wu, Haizhen (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Ye, Jiang (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Wang, Qiyao (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Zhang, Huizhan (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
  • Received : 2019.04.15
  • Accepted : 2019.07.09
  • Published : 2019.08.28
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Abstract

Edwardsiella piscicida is the causative agent of edwardsiellosis, which has caused enormous economic losses worldwide. In our previous research, an attenuated live vaccine known as WED and based on the virulent strain E. piscicida EIB202 can effectively protect turbots against edwardsiellosis via intraperitoneal injection, while vaccination by immersion exhibits a weaker effect. During the development of the immersion vaccine, we surprisingly found the counts of ${\Delta}pEIB202/EIB202$ colonized on zebrafish were 100 times lower than those of EIB202. However, pEIB202 carries 53 predicted ORFs and has several copies in E. piscicida EIB202, impeding the study of its function. Thus, the replication region is located at a 1,980 bp fragment (from 18,837 to 20,816 bp), containing a transcriptional repressor and a replication protein. Moreover, the minimal replication plasmid, named pRep-q77, has low copies in both E. coli and E. piscicida, but is more stable in E. piscicida than in E. coli. This work lays a foundation for further examination of the function of the virulence plasmid pEIB202.

Keywords

References

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