Fig. 1. Polyacrylamide gel showing the RFLP patterns of RNA2 fragment of the Strawberry mottle virus isolates amplified with 2SMoV1108F-2SMoV2386R primers digested with BfaI, FauI, HaeIII, HincI, and TaqI restriction enzymes of strawberry samples collected in 2015 in Bulgaria and Poland. Lanes: L – Thermo Scientific GeneRuler 100 bp Plus DNA Ladder, fragment sizes in base pairs (from top to bottom): 3000, 2000, 1500, 1200, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100. Samples: 1. Unkn-1108, 2. Granda-1108, 3. Markat-1108, 4. Pink-1108, 5. karkas-1108, 6. Pegat-1108, 7. Granat-1108.
Fig. 2. Phylogenic analysis of the RNA2 fragment sequences of the SMoV isolates detected in Poland and nine representative SMoV strains. The SMoV isolates characterized during this study are indicated in bold. Phylogenetic tree was constructed by the maximum likelihood algorithm (MEGA 5.2) and the Kimura 3-parameter nucleotide substitution model in using 1,000 bootstrap replicates. Only bootstrap values higher than 60 are shown. The bar indicates the genetic distance as Kimura units. RNA2 sequence of Satsuma dwarf virus (GenBank accession number: AB009959) was used as the external group.
Table 1. Diversity of the RFLP profiles after digestion of RNA2 fragment of SMoV isolates by BfaI, FauI, HincI, TaqI, and HaeIII enzymes. Designation by A or B or C letters indicates the presence of different restriction patterns for the specific enzyme
참고문헌
- Babini, A. R., Cieslinska, M., Karesova, R., Thompson, J. R. and Cardoni, M. 2004. Occurrence and identification of strawberry viruses in five European countries. Acta Hort. 656:39-43. https://doi.org/10.17660/actahortic.2004.656.4
- Bhagwat, B., Dickison, V., Ding, X., Walker, M., Bernardy, M., Bouthillier, M., Creelman, A., DeYoung, R., Li, Y., Nie, X., Wang, A., Xiang, Y. and Sanfacon, H. 2016. Genome sequence analysis of five Canadian isolates of strawberry mottle virus reveals extensive intra-species diversity and a longer RNA2 with increased coding capacity compared to a previously characterized European isolate. Arch. Virol. 161:1657-1663. https://doi.org/10.1007/s00705-016-2799-6
- Boom, R., Sol, C. J. A., Salimans, M. M. M., Jansen, C. L., Wertheim-van Dillen, P. M. E. and van der Noordaa, J. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495-503. https://doi.org/10.1128/JCM.28.3.495-503.1990
- Cieslinska, M. 2004. Detection of Strawberry mottle virus (SMoV) using RT-PCR-comparison of two RNA extraction methods. J. Fruit Ornam. Plant Res. 12:17-22.
- Cieslinska, M. and Stankiene, J. 2005. The reaction of herbaceous plant species to infection with Strawberry mottle virus isolates occurring in Poland and Lithuania. Phytopathol. Pol. 37:91-97.
- Cieslinska, M. and Zawadzka, B. 1991. Occurrence of virus diseases on strawberries in Poland. Phytopathol. Pol. 12:19-22.
- Cieslinska, M. and Zawadzka, B. 1996. The spread of strawberry mottle virus and its effect on the yield of three strawberry cultivars. J. Fruit Ornam. Plant Res. 4:131-139.
- Frazier, N. W. and Sylvester, E. S. 1960. Half-lives of transmissibility of two aphid-borne viruses. Virology 12:233-244. https://doi.org/10.1016/0042-6822(60)90197-5
- Koloniuk, I., Franova, J., Sarkisova, T. and Přibylova, J. 2018. Complete genome sequences of two divergent isolates of strawberry crinkle virus coinfecting a single strawberry plant. Arch. Virol. 163:2539-2542. https://doi.org/10.1007/s00705-018-3860-4
- Malinowski, T. 1997. Silicacapture-reverse transcriptionpolymerase chain reaction (SC-RT-PCR): application for the detection of several plant viruses. In: Diagnosis and identification of plant pathogens, eds. by H. W. Dehne, G. Adam, M. Diekmann, J. Frahm, A. Mauler-Machnik, and P. van Halteren, pp. 445-448. Springer, Dordrecht, Netherlands.
- Mellor, F. C. and Krczal, H. 1987. Strawberry mottle. In: Virus diseases of small fruits, ed. by R. H. Converse, pp. 10-16. USDA ARS Handbook No. 631, Washington, D.C., USA.
- Sanfacon, H., Wellink, J., Le Gall, O., Karasev, A., van der Vlugt, R. and Wetzel, T. 2009. Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus. Arch. Virol. 154:899-907. https://doi.org/10.1007/s00705-009-0367-z
- Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4:Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599. https://doi.org/10.1093/molbev/msm092
- Thompson, J. R., Leone, G., Lindner, J. L., Jelkmann, W. and Schoen, C. D. 2002. Characterization and complete nucleotide sequence of Strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses. J. Gen. Virol. 83:229-239. https://doi.org/10.1099/0022-1317-83-1-229
- Thompson, J. R. and Jelkmann, W. 2003. The detection and variation of Strawberry mottle virus. Plant Dis. 87:385-390. https://doi.org/10.1094/PDIS.2003.87.4.385
- Thompson, J. R., Wetzel, S., Klerks, M. M., Vaskova, D., Schoen, C. D., Spak, J. and Jelkmann, W. 2003. Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control. J. Virol. Methods 111:85-93. https://doi.org/10.1016/S0166-0934(03)00164-2