International Journal of Stem Cells

  • 제12권2호
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  • Pages.340-346
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  • 2019
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  • 2005-3606(pISSN)
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  • 2005-5447(eISSN)
한국줄기세포학회 (Korean Society for Stem Cell Research)
권호 표지
DOI QR코드

DOI QR Code

Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons

  • Lim, Mi-Sun (Research and Development Center, Jeil Pharmaceutical Company) ;
  • Ko, Seung Hwan (Graduate School of Biomedical Science & Engineering, Hanyang University) ;
  • Kim, Min Sung (School of Mechanical & Aerospace Engineering, Seoul National University) ;
  • Lee, Byungjun (School of Mechanical & Aerospace Engineering, Seoul National University) ;
  • Jung, Ho-Sup (Center for Food and Bioconvergence, Department of Food Science and Biotechnology, Seoul National University) ;
  • Kim, Keesung (Research Institute of Advanced Materials, Seoul National University) ;
  • Park, Chang-Hwan (Graduate School of Biomedical Science & Engineering, Hanyang University)
  • 투고 : 2018.11.29
  • 심사 : 2019.03.10
  • 발행 : 2019.07.31
⟨ 이전 논문 다음 논문 ⟩

초록

The concept of cellular reprogramming was developed to generate induced neural precursor cells (iNPCs)/dopaminergic (iDA) neurons using diverse approaches. Here, we investigated the effects of various nanoscale scaffolds (fiber, dot, and line) on iNPC/iDA differentiation by direct reprogramming. The generation and maturation of iDA neurons (microtubule-associated protein 2-positive and tyrosine hydroxylase-positive) and iNPCs (NESTIN-positive and SOX2-positive) increased on fiber and dot scaffolds as compared to that of the flat (control) scaffold. This study demonstrates that nanotopographical environments are suitable for direct differentiation methods and may improve the differentiation efficiency.

키워드

과제정보

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI16C1013 to Keesung Kim and Chang-Hwan Park) and Basic Science Research Program (NRF2016R1D1A1B03931915 to Mi-Sun Lim).

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