International Journal of Stem Cells

  • 제12권2호
  • /
  • Pages.360-366
  • /
  • 2019
  • /
  • 2005-3606(pISSN)
  • /
  • 2005-5447(eISSN)
한국줄기세포학회 (Korean Society for Stem Cell Research)
권호 표지
DOI QR코드

DOI QR Code

MBP-FGF2-Immobilized Matrix Maintains Self-Renewal and Myogenic Differentiation Potential of Skeletal Muscle Stem Cells

  • Sah, Jay Prakash (Soonchunhyang Institute of Medi-bio Science, Soon Chun Hyang University) ;
  • Hao, Nguyen Thi Thu (Soonchunhyang Institute of Medi-bio Science, Soon Chun Hyang University) ;
  • Kim, Yunhye (Soonchunhyang Institute of Medi-bio Science, Soon Chun Hyang University) ;
  • Eigler, Tamar (Department of Molecular Cell Biology, Weizmann Institute of Science) ;
  • Tzahor, Eldad (Department of Molecular Cell Biology, Weizmann Institute of Science) ;
  • Kim, Sang-Heon (Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology) ;
  • Hwang, Yongsung (Soonchunhyang Institute of Medi-bio Science, Soon Chun Hyang University) ;
  • Yoon, Jeong Kyo (Soonchunhyang Institute of Medi-bio Science, Soon Chun Hyang University)
  • 투고 : 2018.12.06
  • 심사 : 2018.12.20
  • 발행 : 2019.07.31
⟨ 이전 논문 다음 논문 ⟩

초록

The robust capacity of skeletal muscle stem cells (SkMSCs, or satellite cells) to regenerate into new muscles in vivo has offered promising therapeutic options for the treatment of degenerative muscle diseases. However, the practical use of SkMSCs to treat muscle diseases is limited, owing to their inability to expand in vitro under defined cultivation conditions without loss of engraftment efficiency. To develop an optimal cultivation condition for SkMSCs, we investigated the behavior of SkMSCs on synthetic maltose-binding protein (MBP)-fibroblast growth factor 2 (FGF2)-immobilized matrix in vitro. We found that the chemically well-defined, xeno-free MBP-FGF2-immobilized matrix effectively supports SkMSC growth without reducing their differentiation potential in vitro. Our data highlights the possible application of the MBP-FGF2 matrix for SkMSC expansion in vitro.

키워드

과제정보

This research was supported by the Global Research Development Program grant (2016K1A4A3914725) and research grant (2016R1A2B4012956) from the National Research Foundation of Korea (NRF) to JK Yoon and the research grant (HI17C1193) from Korea Health Industry Development Institute (KHIDI) to Y Hwang.

참고문헌

  1. Morgan JE, Partridge TA. Muscle satellite cells. Int J Biochem Cell Biol 2003;35:1151-1156 https://doi.org/10.1016/S1357-2725(03)00042-6
  2. Yin H, Price F, Rudnicki MA. Satellite cells and the muscle stem cell niche. Physiol Rev 2013;93:23-67 https://doi.org/10.1152/physrev.00043.2011
  3. Hill M, Wernig A, Goldspink G. Muscle satellite (stem) cell activation during local tissue injury and repair. J Anat 2003;203:89-99 https://doi.org/10.1046/j.1469-7580.2003.00195.x
  4. Seale P, Rudnicki MA. A new look at the origin, function, and "stem-cell" status of muscle satellite cells. Dev Biol 2000;218:115-124 https://doi.org/10.1006/dbio.1999.9565
  5. Kuang S, Rudnicki MA. The emerging biology of satellite cells and their therapeutic potential. Trends Mol Med 2008;14:82-91 https://doi.org/10.1016/j.molmed.2007.12.004
  6. Charville GW, Cheung TH, Yoo B, Santos PJ, Lee GK, Shrager JB, Rando TA. Ex vivo expansion and in vivo self-renewal of human muscle stem cells. Stem Cell Reports 2015;5:621-632 https://doi.org/10.1016/j.stemcr.2015.08.004
  7. Thomas K, Engler AJ, Meyer GA. Extracellular matrix regulation in the muscle satellite cell niche. Connect Tissue Res 2015;56:1-8 https://doi.org/10.3109/03008207.2014.947369
  8. Bjornson CR, Cheung TH, Liu L, Tripathi PV, Steeper KM, Rando TA. Notch signaling is necessary to maintain quiescence in adult muscle stem cells. Stem Cells 2012;30:232-242 https://doi.org/10.1002/stem.773
  9. Boldrin L, Neal A, Zammit PS, Muntoni F, Morgan JE. Donor satellite cell engraftment is significantly augmented when the host niche is preserved and endogenous satellite cells are incapacitated. Stem Cells 2012;30:1971-1984 https://doi.org/10.1002/stem.1158
  10. Gilbert PM, Havenstrite KL, Magnusson KE, Sacco A, Leonardi NA, Kraft P, Nguyen NK, Thrun S, Lutolf MP, Blau HM. Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture. Science 2010;329:1078-1081 https://doi.org/10.1126/science.1191035
  11. Han WM, Anderson SE, Mohiuddin M, Barros D, Nakhai SA, Shin E, Amaral IF, Pego AP, Garcia AJ, Jang YC. Synthetic matrix enhances transplanted satellite cell engraftment in dystrophic and aged skeletal muscle with comorbid trauma. Sci Adv 2018;4:eaar4008 https://doi.org/10.1126/sciadv.aar4008
  12. Kang JM, Han M, Park IS, Jung Y, Kim SH, Kim SH. Adhesion and differentiation of adipose-derived stem cells on a substrate with immobilized fibroblast growth factor. Acta Biomater 2012;8:1759-1767 https://doi.org/10.1016/j.actbio.2012.01.005
  13. Kang J, Park HM, Kim YW, Kim YH, Varghese S, Seok HK, Kim YG, Kim SH. Control of mesenchymal stem cell phenotype and differentiation depending on cell adhesion mechanism. Eur Cell Mater 2014;28:387-403 https://doi.org/10.22203/eCM.v028a27
  14. Kim JH, Park Y, Jung Y, Kim SH, Kim SH. Combinatorial therapy with three-dimensionally cultured adipose-derived stromal cells and self-assembling peptides to enhance angiogenesis and preserve cardiac function in infarcted hearts. J Tissue Eng Regen Med 2017;11:2816-2827 https://doi.org/10.1002/term.2181
  15. Motohashi N, Asakura Y, Asakura A. Isolation, culture, and transplantation of muscle satellite cells. J Vis Exp 2014 Apr 8 [Epub]. doi: 10.3791/50846
  16. Michailovici I, Harrington HA, Azogui HH, Yahalom-Ronen Y, Plotnikov A, Ching S, Stumpf MP, Klein OD, Seger R, Tzahor E. Nuclear to cytoplasmic shuttling of ERK promotes differentiation of muscle stem/progenitor cells. Development 2014;141:2611-2620 https://doi.org/10.1242/dev.107078
  17. Han XH, Jin YR, Seto M, Yoon JK. A WNT/beta-catenin signaling activator, R-spondin, plays positive regulatory roles during skeletal myogenesis. J Biol Chem 2011;286:10649-10659 https://doi.org/10.1074/jbc.M110.169391
  18. Asfour HA, Allouh MZ, Said RS. Myogenic regulatory factors: the orchestrators of myogenesis after 30 years of discovery. Exp Biol Med (Maywood) 2018;243:118-128 https://doi.org/10.1177/1535370217749494
  19. Humphries JD, Wang P, Streuli C, Geiger B, Humphries MJ, Ballestrem C. Vinculin controls focal adhesion formation by direct interactions with talin and actin. J Cell Biol 2007;179:1043-1057 https://doi.org/10.1083/jcb.200703036
  20. Atherton P, Stutchbury B, Jethwa D, Ballestrem C. Mechanosensitive components of integrin adhesions: role of vinculin. Exp Cell Res 2016;343:21-27 https://doi.org/10.1016/j.yexcr.2015.11.017

피인용 문헌

  1. Heparin-Mimicking Polymer-Based In Vitro Platform Recapitulates In Vivo Muscle Atrophy Phenotypes vol.22, pp.5, 2021, https://doi.org/10.3390/ijms22052488