Fig. 1. MTT analysis in various human cell lines treated with H2O2.
Fig. 2. Analysis of cell viability by MTT assay in 10S (A), 50S (B) and aged 10S (C) fibroblasts treated with H2O2.
Fig. 3. Analysis of cell viability by MTT assay in 10S bone marrow (10S BMSC, A), third molar (10S DSC, B) and adipose (10S, ASC, C) tissue-derived mesenchymal stem cells treated with H2O2.
Fig. 4. Determination of mean IC50 values by MTT assay in 10S fibroblasts, 50S fibroblasts, aged 10S fibroblasts, 10S BMSC, DSC and ASC treated with H2O2. a, b, c and d indicate significant (P<0.05) difference among each cell lines, respectively.
Fig. 5. A. Analysis of population doubling time (PDT) in 10S fibroblasts, 50S fibroblasts, aged 10S fibroblasts, 10S BMSC, DSC and ASC treated with H2O2. a and b indicate significant (P<0.05) difference between untreated control (■) and treatment (■), respectively.
Fig. 6. Morphological alterations and senescence-associated-β-galactosidase activity in 10S fibroblasts, 50S fibroblasts, aged 10S fibroblasts, 10S BMSC, DSC and ASC treated with H2O2 under inverted microscope (×200). The cells with high β-galactosidase activity were stained to blue color. Scale bars; 50 μm.
Fig. 7. Expression level of GPX (■) and CAT (■) transcripts analyzed by RT-PCR in 10S fibroblasts, 50S fibroblasts, aged 10S fibroblasts, 10S BMSC, DSC and ASC. A, B and C indicate significant (P<0.05) difference on GPX transcript among cell lines, respectively. a, b, c and d indicate significant (P<0.05) difference on CAT transcript among cell lines, respectively.
Table 1. Primer sequence for RT-PCR
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