Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Culture condition for gametophyte and sporophyte masspropagation of bamboo fern (Coniogramme japonica) using tissue culture

조직배양을 이용한 가지고비고사리의 전엽체와 포자체 대량번식을 위한 배양조건

  • Park, Kyungtae (Division of Animal, Horticultural and Food Sciences, Chungbuk National University) ;
  • Jang, Bo Kook (Division of Animal, Horticultural and Food Sciences, Chungbuk National University) ;
  • Lee, Cheol Hee (Division of Animal, Horticultural and Food Sciences, Chungbuk National University)
  • 박경태 (충북대학교 축산.원예.식품공학부) ;
  • 장보국 (충북대학교 축산.원예.식품공학부) ;
  • 이철희 (충북대학교 축산.원예.식품공학부)
  • Received : 2019.03.18
  • Accepted : 2019.05.22
  • Published : 2019.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the optimal culture method for gametophyte and sporophyte propagation in Coniogramme japonica (Thunb.) Diels, which can be used in various fields. The propagation of prothallus were cultured in 1/4 - 1 Murashige and Skoog medium and Knop medium for 10 weeks. The results indicated that the fresh weight of prothallus was the highest (14.5 g) in 1MS medium. Subsequently, various concentrations of sucrose, activated charcoal and nitrogen source were also added to 1MS medium and cultured for 8 weeks. The results provided that the sucrose concentration was 3% and the fresh weight of prothallus was the highest 10.8 g. According to the concentration in the range of 8.8 ~ 10.8 g, in the case of activated charcoal, the four treatments showed no significant difference. The nitrogen source was added at a concentration of 30, 60 and 120 mM with the ratio of ${NH_4}^+:{NO_3}^-$ being 1 : 2. As a result, the fresh weight of all treatments increased to similar level but there was no significant difference. We investigated sporophyte formation according to soil type and the highest number of sporophytes at 228.0 was formed in soils mixed with horticultural substrate and decomposed granite at 2 : 1 (v : v). On the other hand, sporophyte was not formed in soils containing peatmoss except for the one with peatmoss and decomposed granite at 2 : 1 (v : v).

본 연구는 다양한 분야에서 활용이 가능한 봉의꼬리과 가지고비고사리[Coniogramme japonica (Thunb.) Diels]의 전엽체와 포자체의 대량번식을 위한 적정 배양방법을 구명하고자 수행되었다. 전엽체 증식을 위해 기내에서 발아하여 계대배양한 전엽체를 재료로 사용하여 1/4-1배로 조절한 MS 배지와 Knop 배지에 10주간 배양한 결과, 1MS 배지에서 전엽체의 생체중이 14.5 g으로 가장 많이 증가하였다. 이후 1MS 배지에 sucrose와 활성탄, 질소급원의 농도를 다르게 조절하여 8주간 배양한 결과, 3%의 sucrose 농도에서 전엽체의 생체중이 10.8 g으로 증식률이 가장 좋았다. 활성탄의 경우 네 처리구가 8.8 ~ 10.8 g 범위로 농도에 따른 유의적인 차이를 보이지 않았다. 질소급원은 ${NH_4}^+:{NO_3}^-$의 비를 1:2로 하여 30, 60, 120 mM의 농도로 첨가한 결과, 세 처리구 모두 생체중에 유의한 차이가 없었으며 비슷한 수준으로 증가하였다. 토양종류에 따른 포자체의 형성을 실험한 결과, 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 228.0개로 가장 많은 포자체가 형성되었다. 반면에 피트모스와 마사토를 2:1(v:v)로 혼합한 토양을 제외하고 피트모스가 첨가된 토양에서는 포자체가 형성되지 않았다.

Keywords

References

  1. Cho JS, Han JH, Lee CH (2017) Effects of medium components and composition on mass propagation of Arachniodes aristata (G. Forst.) Tindale. Korean J Hortic Sci 35:131-141
  2. Cho JS, Lee CH (2017) Several factors affecting mass production of Microlepia strigosa (Thunb.) C. Presl sporophytes. Korean J Hortic Sci 35:46-58
  3. Dyer AF (1979) The Experimental Biology of Ferns. Academic Press, London
  4. Fernandez H, Bertrand AM, Sanchez-Tames R (1999) Biological and nutritional aspects involved in fern multiplication. Plant Cell Tiss Org 56: 211-214 https://doi.org/10.1023/A:1006277229136
  5. Fernandez H, Revilla MA (2003) In vitro culture of ornamental ferns. Plant Cell Tiss Org 73:1-13 https://doi.org/10.1023/A:1022650701341
  6. Grzyb M, Kalandyk A, Waligorski P, Mikula A (2017) The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii sternb. Plant Cell Tiss Org 129:387-397 https://doi.org/10.1007/s11240-017-1185-8
  7. Hirsch AM (1975) The effect of sucrose on the differentiation of excised fern leaf tissue into either gametophytes of sporophytes. Plant Physiol 56:390-393 https://doi.org/10.1104/pp.56.3.390
  8. Jang BK, Cho JS, Kwon HJ, Lee CH (2019a) Optimal conditions for spore germination and gametophyte and sporophyte production in the autumn fern Dryopteris erythrosora. Hortic Environ Biote 60:115-123 https://doi.org/10.1007/s13580-018-0097-9
  9. Jang BK, Cho JS, Lee CH (2019b) Propagation methods for gametophyte proliferation and sporophyte formation in silver cloak fern (Cheilanthes argentea). Hortic Environ Biote 60:435-442 https://doi.org/10.1007/s13580-019-00128-6
  10. Korea Biodiversity Information System (KBIS) (2019) Korea National Arboretum. Pocheon, Korea. Avaliable via http://www.nature.go.kr/kbi/plant/pilbk/selectPlantPilbkDtl.do?plantPilbkNo=35172 (accessed data: 20 Feb. 2019)
  11. Korea Fern Society (KFS) (2005) Illustration Fern Native of Korea. Geobook, Seoul, Korea
  12. Kwon HJ, Han JH, Lee CH, Kim SY (2017) Conditions of in vitro spore germination and prothallium culture for sporophyte propagation of Polystichum braunii (Spenn.) Fee. J Plant Biol 44:454-461
  13. Lee CS, Lee KH (2018) Pteridophytes of Korea: Lycophytes & Ferns. Geobook, Seoul, Korea
  14. Liu Y, Wujisguleng W, Long C (2012) Food uses of ferns in China: A review. Acta Soc Bot Pol 81:263-270 https://doi.org/10.5586/asbp.2012.046
  15. Makowski D, Tomiczak K, Rybczynski JJ, Mikula A (2016) Integration of tissue culture and cryopreservation methods for propagation and conservation of the fern Osmunda regalis L. Acta Physiol Plant 38:19 https://doi.org/10.1007/s11738-015-2037-y
  16. Mikula A, Pozoga M, Tomiczak K, Rybczynski JJ (2015) Somatic embryogenesis in ferns: A new experimental system. Plant Cell Rep 34:783-794 https://doi.org/10.1007/s00299-015-1741-9
  17. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  18. Shin SL, Lee MY, Choi JS, Lee CH (2010) Effect of plant growth regulators on sporophyte formation from in vitro cultured prothallus of bracken fern. Korean J Hortic Sci 28:301-306
  19. Shin SL, Lee CH (2009) In vitro medium composition and culture method affecting masspropagation of Osmunda japonica Thunb. prothalli. Korean J Hortic Sci 27:299-304
  20. Teng WL (1997) Activated charcoal affects morphogenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum. Plant Cell Rep 17:77-83 https://doi.org/10.1007/s002990050356
  21. Thomas TD (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618-631 https://doi.org/10.1016/j.biotechadv.2008.08.003