Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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The Protective Effect of Ethanol Extract of Polygalae Radix against Oxidative Stress-Induced DNA Damage and Apoptosis in Chang Liver Cells

산화적 스트레스에 의한 간세포의 DNA 손상 및 세포사멸 유도에 미치는 원지 에탄올 추출물의 보호 효과

  • Kim, Hong Yun (Department of Internal Medicine, College of Korean Medicine, Dong-Eui University) ;
  • Park, Cheol (Department of Molecular Biology, College of Natural Sciences, Dong-Eui University) ;
  • Choi, Yung Hyun (Department of Biochemistry, College of Korean Medicine, Dong-Eui University) ;
  • Hwang, Won-Deok (Department of Internal Medicine, College of Korean Medicine, Dong-Eui University)
  • 김홍윤 (동의대학교 한의과대학 내과학교실) ;
  • 박철 (동의대학교 자연과학대학 분자생물학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 황원덕 (동의대학교 한의과대학 내과학교실)
  • Received : 2019.04.09
  • Accepted : 2019.05.24
  • Published : 2019.06.30
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Abstract

Objectives: The purpose of the present study was to evaluate the preventive effects of ethanol extract of Polygalae radix (EEPR) against oxidative stress (hydrogen peroxide, $H_2O_2$)-induced DNA damage and apoptosis in Chang liver cells. Methods: Chang liver cells were pretreated with various concentrations of EEPR and then challenged with 0.5 mM $H_2O_2$. The cell viability and apoptosis were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry analysis, respectively. The levels of reactive oxygen species (ROS), mitochondrial membrane potentials (MMPs) and adenosine tri-phosphate (ATP) contents were measured. Expression levels of Bcl-2 and Bax were also determined using Western blot analysis. Results: The results showed that the decreased survival rate induced by $H_2O_2$ could be attributed to the induction of DNA damage and apoptosis accompanied by the increased production of ROS, which was remarkably protected by EEPR. In addition, the loss of $H_2O_2$-induced MMPs and ATP contents was significantly attenuated in the presence of EEPR. The inhibitory effect of EEPR on $H_2O_2$-induced apoptosis was associated with up-regulation of Bcl-2 and down-regulation of Bax, thus reducing the Bax/Bcl-2 ratio. Conclusions: Our data prove that EEPR protects Chang liver cells against $H_2O_2$-induced DNA damage and apoptosis by scavenging ROS and thus suppressing the mitochondrial-dependent apoptosis pathway.

Keywords

References

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