Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Serotype Distribution and Virulence Profile of Salmonella enterica Serovars Isolated from Food Animals and Humans in Lagos Nigeria

  • Abraham, Ajayi (Department of Microbiology, University of Lagos Akoka) ;
  • Stella, Smith (Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research Lagos) ;
  • Ibidunni, Bode-Sojobi (Department of Medical Microbiology, Lagos University Teaching Hospital) ;
  • Coulibaly, Kalpy Julien (Centre Nationale de Reference de Salmonella, Laboratoire de Bacteriologie et Virologie Institut Pasteur de Cote d'Ivoire) ;
  • Funbi, Jolaiya Tolulope (Department of Microbiology, University of Lagos Akoka) ;
  • Isaac, Adeleye Adeyemi (Department of Microbiology, University of Lagos Akoka)
  • Received : 2018.08.16
  • Accepted : 2018.10.26
  • Published : 2019.06.28
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Abstract

Distribution of Salmonella enterica serovars and their associated virulence determinants is wide-spread among food animals, which are continuously implicated in periodic salmonellosis outbreaks globally. The aim of this study was to determine and evaluate the diversity of five Salmonella serovar virulence genes (invA, pefA, cdtB, spvC and iroN) isolated from food animals and humans. Using standard microbiological techniques, Salmonella spp. were isolated from the feces of humans and three major food animals. Virulence determinants of the isolates were assayed using PCR. Clonal relatedness of the dominant serovar was determined via pulsed-field gel electrophoresis (PFGE) using the restriction enzyme, Xbal. Seventy one Salmonella spp. were isolated and serotyped into 44 serovars. Non-typhoidal Salmonella (NTS; 68) accounted for majority (95.8%) of the Salmonella serovars. Isolates from chicken (34) accounted for 47.9% of all isolates, out of which S. Budapest (14) was predominant (34.8%). However, the dominant S. Budapest serovars showed no genetic relatedness. The invA gene located on SPI-1 was detected in all isolates. Furthermore, 94% of the isolates from sheep harbored the spvC genes. The iroN gene was present in 50%, 100%, 88%, and 91% of isolates from human, chicken, sheep, and cattle, respectively. The pefA gene was detected in 18 isolates from chicken and a single isolate from sheep. Notably, having diverse Salmonella serovars containing plasmid encoded virulence genes circulating the food chain is of public health significance; hence, surveillance is required.

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