Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Simultaneous Analysis of Four Standards of The Herbal Formula, DF-02, of Ephedra intermedia and Rheum palmatum, using by High Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UVD)

  • Choi, Seong Yeon (College of Pharmacy, Kangwon National University) ;
  • Jeong, Birang (College of Pharmacy, Kangwon National University) ;
  • Jang, Hyeon Seok (College of Pharmacy, Kangwon National University) ;
  • Lee, Jiho (College of Pharmacy, Kangwon National University) ;
  • Kwon, Yong Soo (College of Pharmacy, Kangwon National University) ;
  • Yoon, Yoosik (Department of Microbiology, College of Medicine, Chung-Ang University) ;
  • Shin, Soon Shik (Department of Formula Sciences, College of Korean Medicine, Dong-Eui University) ;
  • Yang, Heejung (College of Pharmacy, Kangwon National University)
  • Received : 2018.11.11
  • Accepted : 2018.12.09
  • Published : 2019.06.30
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Abstract

The herbal formula, DF-02, consisting of Ephedra intermedia and Rheum palmatum are used for the treatment of the metabolic diseases such as obesity and liver fibrosis in Korean local clinics. We aimed to develop the simultaneous analytical conditions for four standards, (+)-pseudoephedrine (PSEP) and (-)-ephedrine (EP) for E. intermedia, and aloe-emodin (AE) and chrysophanol (CP) for R. palmatum using HPLC-UV techniques. The validated conditions yielded the high precision (relative standard deviation (RSD) < 3.65%) and the recoveries (94 - 106%) using the calibration curves with high linearity ($R^2$ > 0.9994). As a result, four standards of DF-02 were simultaneously determined under the developed method, which will be utilized for the quality control or evaluation of DF-02 and many herbal preparations containing E. intermedia and R. palmatum.

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References

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