Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Production of monoclonal antibodies against VP28 of white spot syndrome virus (WSSV)

White spot syndrome virus (WSSV)의 VP28에 대한 단클론 항체 생산

  • Bang, Ji-hyeong (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Choon-sup (EnbioGene) ;
  • Kim, Jong-Oh (Institute of Fisheries Sciences, Pukyong National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
  • 방지형 (전남대학교 수산생명의학과) ;
  • 김위식 (전남대학교 수산생명의학과) ;
  • 김춘섭 ((주)엔바이로젠) ;
  • 김종오 (부경대학교 수산과학연구소) ;
  • 오명주 (전남대학교 수산생명의학과)
  • Received : 2019.06.10
  • Accepted : 2019.06.18
  • Published : 2019.06.30
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Abstract

We developed and subsequently characterized mouse monoclonal antibodies (MAbs) against recombinant VP28 structural protein (rVP28) of white spot syndrome virus (WSSV). We established six hybridoma clones secreting MAbs against rVP28: 15A11, 20G6, 31H2, 34H6, 38D1 and 43A1. All six MAbs recognized the 25 kDa of protein in gill homogenates of WSSV-infected shrimp by western blot analysis, while no reactivity was observed in gill homogenates of normal shrimp. Moreover, high enzyme-linked immunosorbent assay (ELISA) optical density (OD) values (0.8-2.68) were observed in the hemolymphs from WSSV-infected shrimp, while low OD values (less than 0.24) were recorded in the hemolymphs from normal shrimp, by using these six MAbs produced in this study. These results suggest that these six MAbs are useful for the detection of WSSV.

Keywords

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Fig. 1. Western blot analysis using gill homogenates of WSSV-infected shrimp (a) and normal shrimp (b). M: molecular marker, 1: MAb 15A11, 2: MAb 20G6, 3: MAb 31H2, 4: MAb 34H6, 5: MAb 38D1, 6: MAb 43A1, 7: anti-WSSV VP28 MAb (positive control).

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Fig. 2. ELISA analysis using hemolymphs from WSSVinfected shrimp and normal shrimp and seven MAbs (15A11, 20G6, 31H2, 34H6, 38D1, 43A1, PC (positive control): anti-WSSV VP28 MAb).

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