Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Evaluation of Absorption and Release, Survival Efficiency and Recovery Rate of Transport Medium according to the CLSI M40-A2 Standard

CLSI M40-A2 기준에 따른 수송배지의 흡수 및 배출, 생존 효율, 회수율 평가

  • Ha, Sung-Il (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Suk, Hyun-Soo (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Shin, Jeong-Seob (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Heo, Woong (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital) ;
  • Park, Kang-Gyun (Department of Laboratory Medicine, The Catholic University of Korea, Uijeongbu Saint Mary's Hospital) ;
  • Park, Yeon-Joon (Department of Laboratory Medicine, The Catholic University of Korea, College of Medicine)
  • 하성일 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 석현수 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 신정섭 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 허웅 (가톨릭대학교 서울성모병원 진단검사의학과) ;
  • 박강균 (가톨릭대학교 의정부성모병원 진단검사의학과) ;
  • 박연준 (가톨릭대학교 의과대학 진단검사의학과)
  • Received : 2019.05.03
  • Accepted : 2019.05.25
  • Published : 2019.06.30
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Abstract

The absorption and release capacities, survival efficiency, and recovery rates of five kinds of transport media were evaluated based on the swab elution method (Quantitative) of CLSI (Clinical and Laboratory Standards Institute) M40-A2. Liquid media showed mostly better results than semi-solid media in the three evaluations. The flocked swabs had better ability to absorption and discharge bacteria than the standard swabs. The liquid medium (S4) had the best survival efficiency. Pneumococcal strains with poor growth had a higher survival efficiency and recovery rate in liquid media (S4, S5). The results of microbial recovery showed that S. pyogenes met all the CLSI standards in all media. S. pneumoniae was inadequate in the semi- solid media (S2, S3) and all the remaining media met the criteria. H. influenzae was unsuitable in semi-solid media (S1, S3) and met the criteria in semi-solid medium (S2) and liquid medium (S4, S5). The viability of the H. influenzae, pneumococcal strain causing respiratory disease, was poor in most media. Overgrowth of P. aeruginosa was observed at room temperature. The combination of liquid medium and flocked swab confirmed the best results in the three evaluation methods.

5종류의 수송배지를 흡수 및 배출 능력, 생존 효율, 회수율을 CLSI (Clinical and Laboratory Standards Institute) M40-A2의 Swab elution method (Quantitative) 기준으로 평가하였다. 액체배지가 반유동 배지보다 3가지 평가에서 대부분 우수한 결과값을 보여주었다. Flocked swab이 standard swab 형태보다 균의 흡수 및 배출의 능력 또한 우수하였다. 생존 효율에 대한 평가 결과는 액체배지(S4)가 가장 우수한 결과값을 보였다. 성장이 좋지 않은 S. pneumoniae는 액체배지(S4, S5)에서 생존효율과 회수율이 높았다. 균 회수율 평가 결과는 S. pyogenes는 모든 배지에서 CLSI 기준에 적합하였다. S. pneumoniae는 반유동 배지(S2, S3)에서 부적합하였고, 나머지 배지는 모두 기준에 적합하였다. H. influenzae는 반유동 배지(S1, S3)에서 부적합하였고, 반유동 배지(S2), 액체배지(S4, S5)에서 기준에 적합하였다. 호흡기 질환을 유발하는 S. pneumonia, H. influenzae의 생존 능력은 대부분의 배지에서 좋지 않았다. P. aeruginosa는 실온에서 과성장이 관찰되었다. 액체배지와 flocked swab의 조합이 3가지 평가 방법에서 가장 뛰어난 결과를 평가를 통해 확인하였다.

Keywords

References

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