대한소아치과학회지 (Journal of the korean academy of Pediatric Dentistry)

  • 제46권1호
  • /
  • Pages.85-92
  • /
  • 2019
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  • 1226-8496(pISSN)
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  • 2288-3819(eISSN)
대한소아치과학회 (Korean Academy of Pediatric Dentistry)
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유치 줄기세포에 대한 다양한 규산칼슘계 재료의 세포독성

Cytotoxicity of Various Calcium Silicate-based Materials with Stem Cells from Deciduous Teeth

  • 윤지혜 (원광대학교 치과대학 소아치과학교실) ;
  • 유용욱 (원광대학교 치과대학 구강생화학교실) ;
  • 안은숙 (경복대학교 치위생과) ;
  • 이준 (원광대학교 치과대학 구강악안면외과학교실) ;
  • 안소연 (원광대학교 치과대학 소아치과학교실)
  • Yun, Jihye (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University) ;
  • You, Yong-Ouk (Department of Oral Biochemistry, College of Dentistry, Wonkwang University) ;
  • Ahn, Eunsuk (Department of Dental Hygiene, Kyungbok University) ;
  • Lee, Jun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Wonkwang University) ;
  • An, So-Youn (Department of Pediatric Dentistry, College of Dentistry, Wonkwang University)
  • 투고 : 2018.06.23
  • 심사 : 2018.09.06
  • 발행 : 2019.02.28
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초록

이 연구의 목적은 유치 줄기세포에 대해 다양한 Calcium silicate-based material (CSM)의 세포독성을 비교하고 평가하는 것이다. Retro $MTA^{(R)}$ (RM), $EZ-Seal^{TM}$ (EZ), ENDOCEM $Zr^{(R)}$ (EN)의 powder를 세포 배지를 이용하여 용출시키고 filtering 하였다. 유치 줄기세포가 다양한 농도의 용출액 하 배양되었다. MTS assay를 통해 CSM 용출액이 세포 증식에 미치는 영향을 분석하였고, Flow cytometry analysis를 통해 세포 표현형의 변화 여부를 관찰하였다. 10% 농도의 용출액에서 배양된 유치 줄기세포의 흡광도 값은 RM > EN > EZ 순이었다(p = 0.0439). 그러나 유치 줄기세포는 재료에 관계없이 간엽줄기세포의 표현형을 유지했다. 세 종류의 CSM이 줄기세포 marker를 변형시키진 않았지만, EZ가 RM과 EN보다 세포적합성이 낮음을 알 수 있었다.

The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro $MTA^{(R)}$ (RM), $EZ-Seal^{TM}$ (EZ) and ENDOCEM $Zr^{(R)}$ (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ (p = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.

키워드

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