Evaluation of the Effect of Oriental Medicinal Herbs with Growth Factors on Bone Development using the SD Strain Rat Model in the Growth Period

뼈 발달에 관한 성장인자를 가진 한약재가 성장기 흰쥐 동물모델에 미치는 영향

Abstract

The purpose of this study was to examine the growth plate, femoral bone length, bone mineral density, and blood composition in various experimental animals fed with oriental medicinal herbs containing growth factors. First, the lengths of the bone growth plates of the positive control (PC) group (fed with Astragalus membranaceus) and the Gh-199 and Sh-188 groups were increased when compared to group N. The Gh-199 group showed a greater increase in bone growth when compared with the PC group. In terms of the femoral bone length and bone mineral density, the effect of both Gh-199 and Sh-188 powders were as good as those of the PC group, and the Gh-199 powder showed a positive effect. Conversely, in the PC group, unlike the Gh-199 and Sh-188 groups, the aspartate aminotransferase(AST) and alanine aminotransferase(ALT) activities in the blood were increased, indicating that A. membranaceus is toxic to the body. Both the PC and Sh-188 groups also showed higher insulin-like growth factor-1(IGF-1) activity when compared with the Gh-199 group. Overall, the bone growth plate, femoral bone length, and bone mineral density measurements, and the blood analysis showed positive results in the group treated with Gh-199, and no specific toxicity of the herbal medicine in the body was evident.

본 연구는 성장 유도 인자가 포함된 여러 가지의 한약재의 조성물을 활용하여 성장기 흰쥐에서 골성장판 길이, 대퇴부 길이, 골밀도(Bone mineral density) 및 혈액분석을 통해 키 성장 효과를 검증하고자 한다. 먼저, 골성장판 길이 분석 결과, N군에 비해 PC군과 Gh-199군 및 Sh-188군의 골성장판 길이가 전반적으로 증가하였고, 특히 Gh-199군의 경우 PC군보다 더 우수한 골성장판 길이 성장률을 보였다. 대퇴부 길이 및 골밀도 경우 Gh-199군에서 보다 긍정적인 효과를 나타내었다. 반면에 황기분말을 급여한 PC군의 경우, Gh-199군 및 Sh-188군과 달리 높은 혈중 AST 및 ALT 수치를 나타내었다. 성장호르몬 인자 중 하나인 IGF-1의 결과, PC군과 Sh-188군은 비슷한 경향이었으며, Gh-199군에서 보다 증가한 것으로 나타났다. 따라서, 이상의 결과 골성장판, 대퇴부길이, 골밀도 및 혈액분석 결과 모두 Gh-199군에서 긍정적인 결과를 나타내어 본 실험에 사용한 한약재 조성물은 키 성장에 효과가 있을 것으로 기대된다.

Fig. 2. Fluorescence microscopy method to measure of growth plate from male rats.

Fig. 3. Effects of samples on the femoral bone length in male rats fed Astragalus membranaceus and Gh-199, Sh-188.

Fig. 4. Comparison of bone mineral density (mg HA/ccm) and bone volume/total volume (%) in male rats fed Astragalus membranaceus and Gh-199, Sh-188.

Fig. 5. Comparison on the activities IGF-1 in male rats fed Astragalus membranaceus and Gh-199, Sh-188.

Fig. 6. Comparison on the activities AST and ALT in male rats fed Astragalus membranaceus and Gh-199, Sh-188.

Fig. 1. Ten steps of experimental material manufacturing process.

Table 1. Compositions of experimental diets

Table 2. Experimental design

Table 3. Change in body weight gain and water consumption of rats fed Astragalus membranaceus and Gh-199, Sh-188 in in vivo experiment (I)

Table 4. Change in body weight gain and water consumption of rats fed Astragalus membranaceus and Gh-199, Sh-188 in in vivo experiment (II)