Fig. 3. Primer sets and HRM curve analysis using the plastid intragenic regions of MatK and TrnL-F in the 4 kinds of thistle species. (A) MatK melting curves of sample from each thistle species. (B) TrnL-F and melting curves of sample from each thistle species.
Fig. 1. Sequence alignment of the MatK and TrnL-F chloroplast intergenic region in 4 kinds of thistle species. Korean species (C. japonicum and C. nipponicum), Canadian species (C. arvense) and European species S. marianum).
Fig. 2. Phylogenetic tree showing the genetic diversity of 8 plants of 4 kinds of thistle species. (A) MatK plastid intergenic regions, (B) TrnL-F plastid intergenic regions. The tree was produced using the neighbor-joining method based on intergenic sequences of the plastid intragenic regions of MatK and TrnL-F.
Table 1. List of plant materials used in this study
Table 2. Primer sequences for amplification of the MatK and TrnL-F fragments
Table 3. Primer sequences for HRM analysis of the MatK and TrnL-F fragments
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