Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Improvement of the osteogenic potential of ErhBMP-2-/EGCG-coated biphasic calcium phosphate bone substitute: in vitro and in vivo activity

  • Hwang, Jae-ho (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry) ;
  • Oh, Seunghan (Department of Dental Biomaterials, Institute of Biomaterials-Implant, Wonkwang University School of Dentistry) ;
  • Kim, Sungtae (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry)
  • Received : 2019.02.06
  • Accepted : 2019.03.27
  • Published : 2019.04.30
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Abstract

Purpose: The aim of this study was to evaluate the enhancement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). Methods: The cell viability, differentiation, and mineralization of osteoblasts was tested with ErhBMP-2-/EGCG solution. Coated BCP surfaces were also investigated. Standardized, 6-mm diameter defects were created bilaterally on the maxillary sinus of 10 male New Zealand white rabbits. After removal of the bony windows and elevation of sinus membranes, ErhBMP-2-/EGCG-coated BCP was applied on one defect in the test group. BCP was applied on the other defect to form the control group. The animals were sacrificed at 4 or 8 weeks after surgery. Histologic and histometric analyses of the augmented graft and surrounding tissue were performed. Results: The 4-week and 8-week test groups showed more new bone (%) than the corresponding control groups (P<0.05). The 8-week test group showed more new bone (%) than the 4-week test group (P<0.05). Conclusions: ErhBMP-2-/EGCG-coated BCP was effective as a bone graft material, showing enhanced osteogenic potential and minimal side effects in a rabbit sinus augmentation model.

Keywords

References

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