Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지
DOI QR코드

DOI QR Code

Adjunctive hyperbaric oxygen therapy for irradiated rat calvarial defects

  • An, Heesuk (Department of Prosthodontics, Yonsei University College of Dentistry) ;
  • Lee, Jung-Tae (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry) ;
  • Oh, Seo-Eun (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry) ;
  • Park, Kyeong-mee (Department of Advanced General Dentistry, Yonsei University College of Dentistry) ;
  • Hu, Kyung-Seok (Division in Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Institute, BK21 PLUS Project, Yonsei University College of Dentistry) ;
  • Kim, Sungtae (Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry) ;
  • Chung, Moon-Kyu (Department of Prosthodontics, Yonsei University College of Dentistry)
  • Received : 2018.09.17
  • Accepted : 2019.01.30
  • Published : 2019.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The aim of this study was to conduct a histologic evaluation of irradiated calvarial defects in rats 4 weeks after applying fibroblast growth factor-2 (FGF-2) with hyaluronan or biphasic calcium phosphate (BCP) block in the presence or absence of adjunctive hyperbaric oxygen (HBO) therapy. Methods: Twenty rats were divided into HBO and non-HBO (NHBO) groups, each of which was divided into FGF-2 and BCP-block subgroups according to the grafted material. Localized radiation with a single 12-Gy dose was applied to the calvaria of rats to simulate radiotherapy. Four weeks after applying this radiation, 2 symmetrical circular defects with a diameter of 6 mm were created in the parietal bones of each animal. The right-side defect was filled with the materials mentioned above and the left-side defect was not filled (as a control). All defects were covered with a resorbable barrier membrane. During 4 weeks of healing, 1 hour of HBO therapy was applied to the rats in the HBO groups 5 times a week. The rats were then killed, and the calvarial specimens were harvested for radiographic and histologic analyses. Results: New bone formation was greatest in the FGF-2 subgroup, and improvement was not found in the BCP subgroup. HBO seemed to have a minimal effect on new bone formation. There was tendency for more angiogenesis in the HBO groups than the NHBO groups, but the group with HBO and FGF-2 did not show significantly better outcomes than the HBO-only group or the NHBO group with FGF-2. Conclusions: HBO exerted beneficial effects on angiogenesis in calvarial defects of irradiated rats over a 4-week healing period, but it appeared to have minimal effects on bone regeneration. FGF-2 seemed to enhance new bone formation and angiogenesis, but its efficacy appeared to be reduced when HBO was applied.

Keywords

References

  1. Anderson L, Meraw S, Al-Hezaimi K, Wang HL. The influence of radiation therapy on dental implantology. Implant Dent 2013;22:31-8. https://doi.org/10.1097/ID.0b013e31827e84ee
  2. Granstrom G, Jacobsson M, Tjellstrom A. Titanium implants in irradiated tissue: benefits from hyperbaric oxygen. Int J Oral Maxillofac Implants 1992;7:15-25.
  3. Jacobsson MG, Jonsson AK, Albrektsson TO, Turesson IE. Short- and long-term effects of irradiation on bone regeneration. Plast Reconstr Surg 1985;76:841-50. https://doi.org/10.1097/00006534-198512000-00006
  4. Hu WW, Ward BB, Wang Z, Krebsbach PH. Bone regeneration in defects compromised by radiotherapy. J Dent Res 2010;89:77-81. https://doi.org/10.1177/0022034509352151
  5. Marx RE. Osteoradionecrosis: a new concept of its pathophysiology. J Oral Maxillofac Surg 1983;41:283-8. https://doi.org/10.1016/0278-2391(83)90294-X
  6. Londahl M. Hyperbaric oxygen therapy as adjunctive treatment of diabetic foot ulcers. Med Clin North Am 2013;97:957-80. https://doi.org/10.1016/j.mcna.2013.04.004
  7. Marx RE, Ames JR. The use of hyperbaric oxygen therapy in bony reconstruction of the irradiated and tissue-deficient patient. J Oral Maxillofac Surg 1982;40:412-20. https://doi.org/10.1016/0278-2391(82)90076-3
  8. Thom SR. Hyperbaric oxygen: its mechanisms and efficacy. Plast Reconstr Surg 2011;127 Suppl 1:131S-141S. https://doi.org/10.1097/PRS.0b013e3181fbe2bf
  9. Sawai T, Niimi A, Takahashi H, Ueda M. Histologic study of the effect of hyperbaric oxygen therapy on autogenous free bone grafts. J Oral Maxillofac Surg 1996;54:975-81. https://doi.org/10.1016/S0278-2391(96)90396-1
  10. Marx RE, Ehler WJ, Tayapongsak P, Pierce LW. Relationship of oxygen dose to angiogenesis induction in irradiated tissue. Am J Surg 1990;160:519-24. https://doi.org/10.1016/S0002-9610(05)81019-0
  11. Pitak-Arnnop P, Hemprich A, Dhanuthai K, Pausch NC. A systematic review in 2008 did not show value of hyperbaric oxygen therapy for osteoradionecrosis. J Oral Maxillofac Surg 2010;68:2644-5. https://doi.org/10.1016/j.joms.2010.06.195
  12. Fritz GW, Gunsolley JC, Abubaker O, Laskin DM. Efficacy of pre- and postirradiation hyperbaric oxygen therapy in the prevention of postextraction osteoradionecrosis: a systematic review. J Oral Maxillofac Surg 2010;68:2653-60. https://doi.org/10.1016/j.joms.2010.04.015
  13. Annane D, Depondt J, Aubert P, Villart M, Gehanno P, Gajdos P, et al. Hyperbaric oxygen therapy for radionecrosis of the jaw: a randomized, placebo-controlled, double-blind trial from the ORN96 study group. J Clin Oncol 2004;22:4893-900. https://doi.org/10.1200/JCO.2004.09.006
  14. Tibbles PM, Edelsberg JS. Hyperbaric-oxygen therapy. N Engl J Med 1996;334:1642-8. https://doi.org/10.1056/NEJM199606203342506
  15. Nevins M, Nevins ML, Schupbach P, Kim SW, Lin Z, Kim DM. A prospective, randomized controlled preclinical trial to evaluate different formulations of biphasic calcium phosphate in combination with a hydroxyapatite collagen membrane to reconstruct deficient alveolar ridges. J Oral Implantol 2013;39:133-9. https://doi.org/10.1563/AAID-JOI-D-12-00185
  16. Mangano C, Perrotti V, Shibli JA, Mangano F, Ricci L, Piattelli A, et al. Maxillary sinus grafting with biphasic calcium phosphate ceramics: clinical and histologic evaluation in man. Int J Oral Maxillofac Implants 2013;28:51-6. https://doi.org/10.11607/jomi.2667
  17. Choi H, Park NJ, Jamiyandorj O, Choi KH, Hong MH, Oh S, et al. Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with two concentrations of expressed recombinant human bone morphogenetic protein 2. J Periodontal Implant Sci 2012;42:119-26. https://doi.org/10.5051/jpis.2012.42.4.119
  18. Choi H, Park NJ, Jamiyandorj O, Hong MH, Oh S, Park YB, et al. Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with synthetic cell binding peptide sequences. J Periodontal Implant Sci 2012;42:166-72. https://doi.org/10.5051/jpis.2012.42.5.166
  19. Shin YS, Seo JY, Oh SH, Kim JH, Kim ST, Park YB, et al. The effects of ErhBMP-2-/EGCG-coated BCP bone substitute on dehiscence around dental implants in dogs. Oral Dis 2014;20:281-7. https://doi.org/10.1111/odi.12109
  20. Clergeau LP, Danan M, Clergeau-Guerithault S, Brion M. Healing response to anorganic bone implantation in periodontal intrabony defects in dogs. Part I. Bone regeneration. A microradiographic study. J Periodontol 1996;67:140-9. https://doi.org/10.1902/jop.1996.67.2.140
  21. Hartman GA, Arnold RM, Mills MP, Cochran DL, Mellonig JT. Clinical and histologic evaluation of anorganic bovine bone collagen with or without a collagen barrier. Int J Periodontics Restorative Dent 2004;24:127-35.
  22. Kigami R, Sato S, Tsuchiya N, Yoshimakai T, Arai Y, Ito K. FGF-2 angiogenesis in bone regeneration within critical-sized bone defects in rat calvaria. Implant Dent 2013;22:422-7. https://doi.org/10.1097/ID.0b013e31829d19f0
  23. Anzai J, Kitamura M, Nozaki T, Nagayasu T, Terashima A, Asano T, et al. Effects of concomitant use of fibroblast growth factor (FGF)-2 with beta-tricalcium phosphate (${\beta}$-TCP) on the beagle dog 1-wall periodontal defect model. Biochem Biophys Res Commun 2010;403:345-50. https://doi.org/10.1016/j.bbrc.2010.11.032
  24. Fakhari A, Berkland C. Applications and emerging trends of hyaluronic acid in tissue engineering, as a dermal filler and in osteoarthritis treatment. Acta Biomater 2013;9:7081-92. https://doi.org/10.1016/j.actbio.2013.03.005
  25. Radomsky ML, Aufdemorte TB, Swain LD, Fox WC, Spiro RC, Poser JW. Novel formulation of fibroblast growth factor-2 in a hyaluronan gel accelerates fracture healing in nonhuman primates. J Orthop Res 1999;17:607-14. https://doi.org/10.1002/jor.1100170422
  26. Kawada S, Wada E, Matsuda R, Ishii N. Hyperbaric hyperoxia accelerates fracture healing in mice. PLoS One 2013;8:e72603. https://doi.org/10.1371/journal.pone.0072603
  27. Sawai T, Niimi A, Takahashi H, Ueda M. Histologic study of the effect of hyperbaric oxygen therapy on autogenous free bone grafts. J Oral Maxillofac Surg 1996;54:975-81. https://doi.org/10.1016/S0278-2391(96)90396-1
  28. Sirin Y, Olgac V, Dogru-Abbasoglu S, Tapul L, Aktas S, Soley S. The influence of hyperbaric oxygen treatment on the healing of experimental defects filled with different bone graft substitutes. Int J Med Sci 2011;8:114-25. https://doi.org/10.7150/ijms.8.114
  29. Jan AM, Sandor GK, Iera D, Mhawi A, Peel S, Evans AW, et al. Hyperbaric oxygen results in an increase in rabbit calvarial critical sized defects. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:144-9. https://doi.org/10.1016/j.tripleo.2005.08.032
  30. Jan A, Sandor GK, Brkovic BB, Peel S, Evans AW, Clokie CM. Effect of hyperbaric oxygen on grafted and nongrafted calvarial critical-sized defects. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;107:157-63. https://doi.org/10.1016/j.tripleo.2008.07.010
  31. Jan A, Sandor GK, Brkovic BB, Peel S, Kim YD, Xiao WZ, et al. Effect of hyperbaric oxygen on demineralized bone matrix and biphasic calcium phosphate bone substitutes. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:59-66. https://doi.org/10.1016/j.tripleo.2009.07.036
  32. Wang X, Ding I, Xie H, Wu T, Wersto N, Huang K, et al. Hyperbaric oxygen and basic fibroblast growth factor promote growth of irradiated bone. Int J Radiat Oncol Biol Phys 1998;40:189-96. https://doi.org/10.1016/S0360-3016(97)00563-4

Cited by

  1. Effective administration of cranial drilling therapy in the treatment of fourth degree temporal, facial and upper limb burns at high altitude: A case report vol.8, pp.20, 2019, https://doi.org/10.12998/wjcc.v8.i20.5062
  2. The effect of hyperbaric oxygen therapy on bone macroscopy, composition and biomechanical properties after ionizing radiation injury vol.15, pp.1, 2019, https://doi.org/10.1186/s13014-020-01542-2