Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
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Effect of Hot Water and Lime-Sulfur Mixture Treatment for Disinfection of Seeds for Organic Lettuce

유기농 상추 재배를 위한 온탕침지와 석회유황합제의 종자소독 효과

  • Kim, Min-Jeong (Organic Agriculture Division, National Institute of Agricultural Sciences, RDA) ;
  • Shim, Chang-Ki (Organic Agriculture Division, National Institute of Agricultural Sciences, RDA) ;
  • Ko, Byeong-Gu (Organic Agriculture Division, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Ju (Organic Agriculture Division, National Institute of Agricultural Sciences, RDA) ;
  • Park, Jong-Ho (Organic Agriculture Division, National Institute of Agricultural Sciences, RDA) ;
  • Yoon, Ji-Young (Organic Agriculture Division, National Institute of Agricultural Sciences, RDA)
  • 김민정 (국립농업과학원 농업환경부 유기농업과) ;
  • 심창기 (국립농업과학원 농업환경부 유기농업과) ;
  • 고병구 (국립농업과학원 농업환경부 유기농업과) ;
  • 김주 (국립농업과학원 농업환경부 유기농업과) ;
  • 박종호 (국립농업과학원 농업환경부 유기농업과) ;
  • 윤지영 (국립농업과학원 농업환경부 유기농업과)
  • Received : 2019.03.16
  • Accepted : 2019.05.29
  • Published : 2019.06.30
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Abstract

The purpose of this study was to investigate the effect of hot water treatment and pH corrected lime sulfur combination treatment on the fungicidal and bacterial disinfection effects and germination rate of organic lettuce seeds. Among the followers, Alternaria sp. was infected 53.3% and Aspergillus sp. and Cladosporium sp. Infected 14.5% and 5.4%, respectively. Bacteria were isolated only Pseudomonas sp., and infected with 16.5%. In order to investigate the effect of disinfection on the temperature of hot water (45℃, 50℃, 55℃ and 60℃). The seed germination rate sharply decreased with increasing temperature and treatment time. The germination effect and germination rate of the follower were highest when hot water treatment was carried out for 20 minutes in hot water at 50℃. In the case of combined treatment of 50℃ hot water for 10 min and 0.4% pH adjusted lime sulfur mixture, showed the highest sterilization effect and germination rate with 100% and 97.6%, respectively. The results of this study can contribute to the development of technology to sterilize not only seed surface but also fungi and bacteria inside of seed.

본 연구의 목적은 온탕침지와 pH교정석회유황합제 처리에 의한 상추 유기종자의 곰팡이와 세균의 소독효과 및 발아율에 미치는 영향을 검정하고자 하였다. 상추종자에 Alternaria sp.가 53.3% 감염되어 있었고 Aspergillus sp.와 Cladosporium sp.은 각각 14.5%와 5.4% 감염되어 있었다. 세균은 형태적으로 Pseudomonas sp., 한 종만 분리되었으며 16.5%가 감염되어 있었다. 상추종자 소독에 효과적인 온탕침지조건을 탐색하고자 온탕의 온도조건(45℃, 50℃, 55℃, 60℃)에 따른 소독효과를 조사하였다. 온탕침지 온도와 처리시간이 증가함에 따라 곰팡이와 세균에 대한 살균효과는 증가하였으나 종자의 발아율은 급격히 감소하였다. 온탕침지 단독처리는 50℃ 온탕에서 20분간 처리할 경우 상추종자의 살균효과와 발아율이 각각 91.1%로 가장 우수하였다. 상추종자 소독을 위한 온탕침지와 pH교정석회유황합제교호처리는 50℃의 온탕침지 조건에서는 10분간 처리하는 것이 상추종자의 살균효과와 발아율이 각각 100%와 97.6%로 가장 높았다. 본 연구결과는 종자 표면뿐만 아니라 종자내의 곰팡이나 세균을 살균할 수 있는 기술개발에 기여할 수 있을 것으로 생각한다.

Keywords

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