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단위동물 모델에서 In vitro 소화율 측정과 평가

Evaluation and Method of In Vitro Digestibility in Monogastric Animal Model

  • 강령인 (경상대학교 응용생명과학부(BK21 Plus) 축산생명학과(농업생명과학연구원)) ;
  • 김진선 (경상대학교 응용생명과학부(BK21 Plus) 축산생명학과(농업생명과학연구원)) ;
  • 이성실 (경상대학교 응용생명과학부(BK21 Plus) 축산생명학과(농업생명과학연구원)) ;
  • 추교문 (농협사료 사료기술연구소) ;
  • 김진욱 (경상대학교 응용생명과학부(BK21 Plus) 축산생명학과(농업생명과학연구원))
  • Kang, Lyeongin (Dept. of Animal Science & Biotechnology, Division of Applied Life Science(BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Jin Seon (Dept. of Animal Science & Biotechnology, Division of Applied Life Science(BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Sung Sill (Dept. of Animal Science & Biotechnology, Division of Applied Life Science(BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Chu, Gyo-Moon (Feed Technology Research Center, Nonghyup Feed Co., Ltd) ;
  • Kim, Jin Wook (Dept. of Animal Science & Biotechnology, Division of Applied Life Science(BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 투고 : 2018.10.15
  • 심사 : 2019.01.16
  • 발행 : 2019.04.30

초록

In vivo 측정법은 비용이 많이 들고 연구기간이 길며 동물복지 측면에서 여러 문제점이 있다. 이에 대한 대안으로 지난 수십년간 in vitro 소화율 측정법이 개발되어 왔다. 본 총설에서는 대표적인 단위 동물로써 사양되고 있는 돼지의 소화기관 특징 및 소화작용에 대한 논하였다. 또한, 최근에 개발된 daisy II incubator 를 이용한 in vitro 방법을 통해 돼지에서 주요 곡류사료로 이용되고 있는 옥수수, 쌀, 소맥 및 대맥의 회장 및 전분 건물소화율을 측정하였다.

An in vitro digestibility methods have been developed to mimic an in vivo system in the past decades. Because the in vivo techniques cause high cost, intensive labor, longer research periods and ethnical problems. In this review, the digestive systems from stomach to large intestine of pig as a monogastric animal were addressed to understand an in vivo digestion. The innovative in vitro technique using the Daisy II incubator was performed and the in vitro ileal and fecal dry matter (DM) digestibility of corn, rice, wheat and barley was determined.

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