Journal of Microbiology and Biotechnology

  • 제29권4호
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  • Pages.538-547
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  • 2019
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Opposite Effects of Vitamin C and Vitamin E on the Antifungal Activity of Honokiol

  • Sun, Lingmei (Department of Pharmacology, Medical School of Southeast University) ;
  • Ye, Xiaolong (Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University) ;
  • Ding, Dafa (Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing Medical University) ;
  • Kai, Liao (Department of Pathology and Pathophysiology, Medical School of Southeast University)
  • 투고 : 2019.01.07
  • 심사 : 2019.03.02
  • 발행 : 2019.04.28
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초록

The aim of the present study was to evaluate the effects of two well-known natural antioxidants, vitamin C (VC) and vitamin E (VE), on the antifungal activity of honokiol against Candida albicans. The broth microdilution method was employed to test the antifungal activities of honokiol with or without antioxidants in the medium against C. albicans strain. Intracellular reactive oxygen species and lipid peroxidation were determined by fluorescence staining assay. Mitochondrial dysfunction was assessed by detecting the mitochondrial DNA and the mitochondrial membrane potential. We observed that VC could significantly potentiate the antifungal activities of honokiol while VE reduced the effectiveness of honokiol against C. albicans. In addition, VC accelerated honokiol-induced mitochondrial dysfunction and inhibited glycolysis leading to a decrease in cellular ATP. However, VE could protect against mitochondrial membrane lipid peroxidation and rescue mitochondrial function after honokiol treatment. Our research provides new insight into the understanding of the action mechanism of honokiol and VC combination against C. albicans.

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