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Pueraria montana var. lobata Root Extract Inhibits Photoaging on Skin through Nrf2 Pathway

  • Heo, Hee Sun (Department of Food Science and Biotechnology, College of Life Sciences, CHA University) ;
  • Han, Ga Eun (Department of Food Science and Biotechnology, College of Life Sciences, CHA University) ;
  • Won, Junho (Department of Food Science and Biotechnology, College of Life Sciences, CHA University) ;
  • Cho, Yeonoh (Department of Food Science and Biotechnology, College of Life Sciences, CHA University) ;
  • Woo, Hyeran (Department of Food Science and Biotechnology, College of Life Sciences, CHA University) ;
  • Lee, Jong Hun (Department of Food Science and Biotechnology, College of Life Sciences, CHA University)
  • 투고 : 2018.12.11
  • 심사 : 2019.03.12
  • 발행 : 2019.04.28

초록

Pueraria montana var. lobata is a bioactive substance with various beneficial health effects and has long been extensively used as a traditional medication for the treatment of fever, acute dysentery, diabetes, and cardiovascular diseases in Northeast Asian countries. The purpose of this study was to evaluate the cytoprotective activity of Pueraria montana var. lobata ethanol extract (PLE) for ultraviolet B (UVB)-induced oxidative stress in human dermal fibroblasts (HDF). It was hypothesized that PLE treatment ($25-100{\mu}g/ml$) would reduce intracellular reactive oxygen species (ROS) levels as well as increase collagen production in UVB-irradiated HDF. The results confirmed this theory, with collagen production increasing in the PLE treatment group in a dose-dependent manner. In addition, regulators of cellular ROS accumulation, including HO-1 and NOQ-1, were activated by Nrf2, which was mediated by PLE. Hence, intracellular levels of ROS were also reduced in the PLE treatment group in a dose-dependent manner. In conclusion, PLE increases collagen production and maintains hyaluronic acid (HA) levels in human dermal fibroblasts exposed to UVB-irradiation, thereby inhibiting photoaging.

키워드

참고문헌

  1. Baumann L. 2007. Skin ageing and its treatment. J. Pathol. 211: 241-251. https://doi.org/10.1002/path.2098
  2. Debacq-Chainiaux F, Leduc C, Verbeke A, Toussaint O. 2012. UV, stress and aging. Dermatoendocrinol. 4: 236-240. https://doi.org/10.4161/derm.23652
  3. Makrantonaki E, Bekou V, Zouboulis CC. 2012. Genetics and skin aging. Dermatoendocrinol. 4: 280-284. https://doi.org/10.4161/derm.22372
  4. Wlaschek M, Tantcheva-Poor I, Naderi L, Ma W, Schneider LA, Razi-Wolf Z, et al. 2001. Solar UV irradiation and dermal photoaging. J. Photochem. Photobiol. B. 63: 41-51. https://doi.org/10.1016/S1011-1344(01)00201-9
  5. Ma W, Wlaschek M, Tantcheva-Poor I, Schneider LA, Naderi L, Razi-Wolf Z, et al. 2001. Chronological ageing and photoageing of the fibroblasts and the dermal connective tissue. Clin. Exp. Dermatol. 26: 592-599. https://doi.org/10.1046/j.1365-2230.2001.00905.x
  6. Yaar M, Eller MS, Gilchrest BA. 2002. Fifty years of skin aging. J. Investig. Dermatol. Symp. Proc. 7: 51-58. https://doi.org/10.1046/j.1523-1747.2002.19636.x
  7. Helfrich YR, Sachs DL, Voorhees JJ. 2008. Overview of skin aging and photoaging. Dermatol. Nurs. 20: 177-183; quiz 184.
  8. Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, et al. 1996. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379: 335-339. https://doi.org/10.1038/379335a0
  9. Kim J, Lee C-W, Kim EK, Lee S-J, Park N-H, Kim H-S, et al. 2011. Inhibition effect of Gynura procumbens extract on UVB-induced matrix-metalloproteinase expression in human dermal fibroblasts. J. Ethnopharmacol. 137: 427-433. https://doi.org/10.1016/j.jep.2011.04.072
  10. Kohl E, Steinbauer J, Landthaler M, Szeimies RM. 2011. Skin ageing. J. Eur. Acad. Dermatol. Venereol. 25: 873-884. https://doi.org/10.1111/j.1468-3083.2010.03963.x
  11. Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M. 2016. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int. J. Mol. Sci. 17(6).
  12. Espinosa-Diez C, Miguel V, Mennerich D, Kietzmann T, Sanchez-Perez P, Cadenas S, et al. 2015. Antioxidant responses and cellular adjustments to oxidative stress. Redox. Biol. 6: 183-197. https://doi.org/10.1016/j.redox.2015.07.008
  13. Loft S, Poulsen HE. 1996. Cancer risk and oxidative DNA damage in man. J. Mol. Med (Berl). 74: 297-312. https://doi.org/10.1007/BF00207507
  14. Rabilloud T, Chevallet M, Luche S, Leize-Wagner E. 2005. Oxidative stress response: a proteomic view. Expert Rev. Proteomics. 2: 949-956. https://doi.org/10.1586/14789450.2.6.949
  15. Zhang DD. 2006. Mechanistic studies of the Nrf2-Keap1 signaling pathway. Drug Metab. Rev. 38: 769-789. https://doi.org/10.1080/03602530600971974
  16. Giudice A, Arra C, Turco MC. 2010. Review of molecular mechanisms involved in the activation of the Nrf2-ARE signaling pathway by chemopreventive agents. Methods Mol. Biol. 647: 37-74. https://doi.org/10.1007/978-1-60761-738-9_3
  17. Kumar H, Kim IS, More SV, Kim BW, Choi DK. 2014. Natural product-derived pharmacological modulators of Nrf2/ARE pathway for chronic diseases. Nat. Prod. Rep. 31: 109-139. https://doi.org/10.1039/C3NP70065H
  18. Dinkova-Kostova AT, Talalay P. 2008. Direct and indirect antioxidant properties of inducers of cytoprotective proteins. Mol. Nutr. Food Res. 52 Suppl 1: S128-138.
  19. Lee JH, Khor TO, Shu L, Su ZY, Fuentes F, Kong AN. 2013. Dietary phytochemicals and cancer prevention: Nrf2 signaling, epigenetics, and cell death mechanisms in blocking cancer initiation and progression. Pharmacol. Ther. 137: 153-171. https://doi.org/10.1016/j.pharmthera.2012.09.008
  20. He CH, Gong P, Hu B, Stewart D, Choi ME, Choi AM, et al. 2001. Identification of activating transcription factor 4 (ATF4) as an Nrf2-interacting protein. Implication for heme oxygenase-1 gene regulation. J. Biol. Chem. 276: 20858-20865. https://doi.org/10.1074/jbc.M101198200
  21. Li N, Alam J, Venkatesan MI, Eiguren-Fernandez A, Schmitz D, Di Stefano E, et al. 2004. Nrf2 is a key transcription factor that regulates antioxidant defense in macrophages and epithelial cells: protecting against the proinflammatory and oxidizing effects of diesel exhaust chemicals. J. Immunol. 173: 3467-3481. https://doi.org/10.4049/jimmunol.173.5.3467
  22. Siegel D, Bolton EM, Burr JA, Liebler DC, Ross D. 1997. The reduction of alpha-tocopherolquinone by human NAD(P)H: quinone oxidoreductase: the role of alpha-tocopherolhydroquinone as a cellular antioxidant. Mol. Pharmacol. 52: 300-305. https://doi.org/10.1124/mol.52.2.300
  23. Bebrevska L, Foubert K, Hermans N, Chatterjee S, Van Marck E, De Meyer G, et al. 2010. In vivo antioxidative activity of a quantified Pueraria lobata root extract. J. Ethnopharmacol. 127: 112-117. https://doi.org/10.1016/j.jep.2009.09.039
  24. Park HJ, Cho DH, Kim HJ, Lee JY, Cho BK, Bang SI, et al. 2009. Collagen synthesis is suppressed in dermal fibroblasts by the human antimicrobial peptide LL-37. J. Invest. Dermatol. 129: 843-850. https://doi.org/10.1038/jid.2008.320
  25. Pawlikowska-Pawlęga B, Ignacy Gruszecki W, Misiak L, Paduch R, Piersiak T, Zarzyka B, et al. 2007. Modification of membranes by quercetin, a naturally occurring flavonoid, via its incorporation in the polar head group. Biochim. Biophys. Acta 1768: 2195-2204. https://doi.org/10.1016/j.bbamem.2007.05.027
  26. Matzinger M, Fischhuber K, Heiss EH. 2018. Activation of Nrf2 signaling by natural products-can it alleviate diabetes? Biotechnol. Adv. 36: 1738-1767. https://doi.org/10.1016/j.biotechadv.2017.12.015
  27. Kwon JE, Lim J, Bang I, Kim I, Kim D, Kang SC. 2019. Fermentation product with new equol-producing Lactobacillus paracasei as a probiotic like product candidate for prevention of skin and intestinal disorder. J. Sci. Food Agric. doi: 10.1002/jsfa.9648. [Epub ahead of print]
  28. Lee J-H, Jeon Y-D, Lee Y-M, Kim D-K. 2018. The suppressive effect of puerarin on atopic dermatitis-like skin lesions through regulation of inflammatory mediators in vitro and in vivo. Biochem. Biophys. Res. Commun. 498: 707-714. https://doi.org/10.1016/j.bbrc.2018.03.018
  29. Han E, Chang B, Kim D, Cho H, Kim S. 2015. Melanogenesis inhibitory effect of aerial part of Pueraria thunbergiana in vitro and in vivo. Arch. Dermatol. Res. 307: 57-72. https://doi.org/10.1007/s00403-014-1489-z

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