황칠나무 발효 추출물의 육모효과

The Effect of Fermented Extracts of Korean Dendropanax Morbifera Levéille on Hair Growth

Abstract

이전 연구에서 우리는 Lactobacillus plantarum ilchiwhangchil 1785와 Lactobacillus plantarum ilchiwhangchil 2020 균주로 발효시킨 황칠나무 잎 가지 추출물의 효과적인 항균 활성을 확인하였다. 본 연구에서는 황칠나무 잎 가지 추출물을 L. plantarum ilchiwhangchil 1785와 L. plantarum ilchiwhangchil 2020 균주로 발효시킨 발효액이 인간모유두세포에 미치는 육모효과를 알아보았다. 황칠나무 잎 가지 발효 추출물의 농도가 1, $10{\mu}g/ml$ 범위에서 인간모유두세포는 세포사멸 증가 없이 유의적으로 세포 성장이 농도의존적으로 증가하였다. 또한 황칠나무 잎 가지 발효물로 처리한 세포가 대조군보다 세포성장이 활발함을 이중염색법으로 확인하였다. 더욱이 황칠나무 잎 가지 발효 추출물 처리한 세포는 처리하지 않은 세포에 비해 각각 18.84%, 23.31% 세포 이동성이 증가하였다. 고성능액체크로마토그래피(HPLC)를 이용하여 육모효과의 활성 물질을 분석하였다. 모발성장효과가 알려진 ${\beta}$-sitosterol 함량이 황칠나무 잎 가지 추출물에 비해 황칠나무 잎 가지 발효 과정에서 약 10배 증가되는 것을 확인하였다. 결과를 종합하면, 우리는 본 연구에서 황칠나무 잎 가지 추출물 발효액이 모발 성장을 촉진시킴을 확인하였다.

In previous studies, we confirmed the effective antimicrobial activity of fermented Dendropanax morbifera leaf/branch extracts with Lactobacillus plantarum ilchiwhangchil 1785 and Lactobacillus plantarum ilchiwhangchil 2020. In this study, we investigated the hair growth effect of D. morbifera leaf/branch extracts fermented with L. plantarum ilchiwhangchil 1785 and L. plantarum ilchiwhangchil 2020 on human hair dermal papilla cells. The growth rate of human hair dermal papilla cells treated with fermented extracts in the range of 1 to $10{\mu}g/ml$ significantly increased in a concentration-dependent manner, without increasing cell death. Double staining studies showed that the growth of cells treated with fermented D. morbifera leaf/branch extracts was more active than that of control cells. Moreover, the cells treated with the fermented D. morbifera leaf/branch extracts exhibited a 18.84% and 23.31% increase in cell mobility, respectively, as compared with that of the untreated cells. High-performance liquid chromatography (HPLC) was used to determine the active agents responsible for hair growth. The results showed that the content of ${\beta}$-sitosterol, which is known to affect hair growth, increased about 10 times in the fermentation process of D. morbifera leaf/branch extracts. Taken together, the findings confirm that fermented Dendropanax morbifera leaf/branch extracts promote hair growth.

Fig. 1. Cell viability of human hair dermal papilla cells with Dendropanax morbifera leave/branch extracts, fermented Dendropanax morbifera leave/branch extracts with Lactobacillus plantarum ilchiwhangchil 1785, and Lactobacillus plantarum ilchiwhangchil 2020. Minoxidil (0.1 μg/ml) was used as a positive control. Data are represented as mean±SEM of three independent experiments. P-values: *p<0.05, **p<0.01, ***p<0.001.

Fig. 2. Visual identification for living and dead cells of human hair dermal papilla cells by fluorescent double staining after 24hr incubation (A) in the absence of fermented Dendropanax morbifera leave/branch extracts, (B) in the presence of fermented Dendropanax morbifera leave/branch extracts with Lactobacillus plantarum ilchiwhangchil 1785, (C) in the presence of fermented Dendropanax morbifera leave/branch extracts with Lactobacillus plantarum ilchiwhangchil 2020, and (D) in the presence of minoxidil (0.1 μg/ml).

Fig. 3. Human dermal hair papilla cells migration test during 24 hr incubation, (A) optical images and (B) analytical data. Minoxidil (0.1 μg/ml) was used as a positive control. Data are represented as mean ± SEM of three independent experiments. P-values: *p<0.05, **p<0.01, ***p<0.001.

Fig. 4. HPLC analysis of β-sitosterol, (A) Raw HPLC data at various concentration of authentic β-sitosterol, (B) calibration curve of β-sitosterol, (C) β-sitosterol analysis of Dendropanax morbifera leave/branch extracts and fermented Dendropanax morbifera leave/branch extracts. *The β-sitosterol peaks (retention time, 2.7 min) are shown as an arrow in A and C.