생명과학회지 (Journal of Life Science)

  • 제29권4호
  • /
  • Pages.442-446
  • /
  • 2019
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

산국 에센셜오일을 함유한 화장품의 주름개선 임상효능에 관한 연구

A Study on the Anti-wrinkle Properties of Cosmetics Containing Essential Oil from Chrysanthemum boreale MAKINO

  • 최인호 (호서대학교 화장품생명공학부) ;
  • 황대일 (호서대학교 화장품생명공학부) ;
  • 김도윤 (호서대학교 화장품생명공학부) ;
  • 김하빈 (호서대학교 화장품생명공학부) ;
  • 이환명 (호서대학교 화장품생명공학부)
  • Choi, In Ho (Division of Cosmetic and Biotechnology, College of Life and Health Science, Hoseo University) ;
  • Hwang, Dae Il (Division of Cosmetic and Biotechnology, College of Life and Health Science, Hoseo University) ;
  • Kim, Do Yoon (Division of Cosmetic and Biotechnology, College of Life and Health Science, Hoseo University) ;
  • Kim, Ha Bin (Division of Cosmetic and Biotechnology, College of Life and Health Science, Hoseo University) ;
  • Lee, Hwan Myung (Division of Cosmetic and Biotechnology, College of Life and Health Science, Hoseo University)
  • 투고 : 2018.10.29
  • 심사 : 2018.12.15
  • 발행 : 2019.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

산국 에센셜오일의 채취시기별 특성과 in vitro 항산화, 미백, 피부재생 및 주름개선 활성이 본 연구진에 의해 최근 보고되었다. 본 연구는 산국 에센셜오일(0.1%, v/v)이 함유된 크림제형의 화장품에 대해 주름개선 효능 및 안전성을 확인하였다. 피시험자는 자연주름이 형성되어 있는 42세에서 60세까지의 22명의 여성을 대상으로 8주 동안 산국 에센셜오일 함유 화장품을 눈가주변에 도포하였다. 인체 피부 안전성은 피부과 전문의의 육안관찰 소견을 확보하고, 피부 주름개선 효능은 visiometer를 이용하여 roughness를 측정 하였다. 산국 에센셜오일 함유 화장품 및 대조시료는 시험기간 동안 인체에 홍반, 알러지 등의 이상반응을 유발하지 않았으며, 시험부위(test)는 대조부위(placebo)에 비해 roughness 지수 감소의 폭이 더 우수한 것으로 확인되었다. 또한 R1, R2, R3 지수는 크림 사용 2주 후부터 시험부위가 대조부위에 비해 통계적으로 유의(p<0.05)한 감소를 나타내었다. 따라서 "산국에센셜오일 함유 화장품"은 인체 안전성이 확보되었으며, 피부주름 개선 활성을 나타내는 것으로 확인되어 향후 비고시 주름개선 기능성화장품으로 개발이 가능할 것으로 사료된다.

We recently reported the characteristics of Chrysanthemum boreale MAKINO essential oil (CBMEO) at different harvesting stages and its in vitro antioxidant, whitening, skin regeneration, and antiwrinkle properties. In the present study, we investigated the effectiveness of cosmetic creams containing 0.1% of CBMEO in terms of wrinkle reduction and safety. The participants were 22 women aged 42 to 60 y with natural wrinkles. All the participants applied the CBMEO-containing cream to the eye area for 8 weeks. Visual observations of the skin by a dermatologist were used to assess the safety of the product, and improvements in skin wrinkles (roughness) were analyzed using a skin visiometer. Neither the CBMEO-containing cream nor the placebo caused abnormal reactions, such as erythema or allergies, during the test period. The CBMEO-containing cream-exposed test regions showed a greater reduction in the roughness index as compared with those of the placebo-exposed control regions. The R1, R2, and R3 indices showed a statistically significant decrease in the test regions as compared with the placebo control regions after using the cream for 2 weeks (p<0.05). Therefore, this study demonstrated that CBMEO-containing cosmetics are safe for human use and that they have skin wrinkle improvement properties. In the future, CBMEO may be a promising product for use in the development of functional anti-wrinkle cosmetics.

키워드

Table 1. Basic information for the Participant

SMGHBM_2019_v29n4_442_t0001.png 이미지

Table 2. Parameters used in assessment of Skin Visiometer SV 600

SMGHBM_2019_v29n4_442_t0002.png 이미지

Table 3. The result of Visiometer SV600–Roughness for test and placebo (8 weeks)

SMGHBM_2019_v29n4_442_t0003.png 이미지

Table 4. Statistical values for variation of Visiometer SV600–Roughness - Comparative evaluation with placebo

SMGHBM_2019_v29n4_442_t0004.png 이미지

Table 5. Abnormal symptoms of skin during test period

SMGHBM_2019_v29n4_442_t0005.png 이미지

참고문헌

  1. Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods a review. Int. J. Food Microbiol. 94, 223-253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  2. Choi, S. K., Park, K. D., Kim, D. A., Lee, D. W. and Kim, Y. J. 2014. Preparation of camel milk liposome and its antiaging effects. J. Soc. Cosmet. Scientists Korea 40, 155-162. https://doi.org/10.15230/SCSK.2014.40.2.155
  3. Figueiredo, A. C., Barroso, J. G., Pedro, L. G. and Scheffer, J. J. C. 2008. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour Frag. J. 23, 213-226. https://doi.org/10.1002/ffj.1875
  4. Kang, S. J., Kim, A. J., Lee, M. S. and Lee, Y. H. 2010. Anti-wrinkle effect of oriental medicine cosmetics containing black ginseng. JKAIS. 11, 3325-3329.
  5. Kim, B. S., Park, S. J., Kim, M. K., Kim, Y. H., Lee, S. B., Lee, K. H., Choi, N. Y., Lee, Y. R., Lee, Y. E. and You, Y. O. 2015. Inhibitory effects of Chrysanthemum boreale essential oil on biofilm formation and virulence factor expression of Streptococcus mutans. Evid.-based Complement Altern. Med. 2015, 1-11.
  6. Kim, D. Y. 2016. Study on characterization and biological activities of essential oil from Korean indigenous plants. Ph. D. dissertation, Hoseo University, Asan, Korea.
  7. Kim, D. Y., Hwang, D. I., Yoon, M. S., Choi, I. H. and Lee, H. M. 2016. Effect of hydrosol extracted from chrysanthemum boreale Makino flower on proliferation and migration in human skin keratinocyte. J. Soc. Cosmet. Scientists Korea 42, 95-101. https://doi.org/10.15230/SCSK.2016.42.1.95
  8. Kim, D. Y., Won, K. J., Hwang, D. I., Park, S. M., Kim, B. and Lee, H. M. 2018. Chemical composition, antioxidant and anti-melanogenic activities of essential oils from Chrysranthemum boreale makino at different harvesting stages. Chem. Biodivers. 15, 1-13.
  9. Kim, D. Y., Won, K. J., Yoon, M. S., Hwang, D. I., Yoon, S. W., Park, J. H., Kim, B. and Lee, H. M. 2015. Chrysanthemum boreale flower floral water inhibits platelet-derived growth factor-stimulated migration and proliferation in vascular smooth muscle cells. Pharm. Biol. 53, 725-734. https://doi.org/10.3109/13880209.2014.941882
  10. Kim, D. Y., Won, K. J., Yoon, M. S., Hwang, D. I., Yoon, S. W., Park, J. H., Kim, B. and Lee, H. M. 2015. Chrysanthemum boreale Makino essential oil induces keratinocyte proliferation and skin regeneration. Nat. Prod. Res. 29, 562-564. https://doi.org/10.1080/14786419.2014.952231
  11. Kim, J. M., Jeon, S. W., Lee, W. G., Nam, H. J. and Kim, Y. B. 2010. Study of preventing methods for skin aging and wrinkles. J. Physiol. Pathol. Kor. Med. 24, 533-542.
  12. Kim, W. S., Park, B. S., Park, S. H., Kim, H. K. and Sung, J. H. 2009. Anti-wrinkle effect of adipose-derived stem cell: activation of dermal fibroblast by secretory factors. J. Dermatol. Sci. 53, 96-102. https://doi.org/10.1016/j.jdermsci.2008.08.007
  13. Lee, H. H. 1999. The study of ageing phenomena and Anti-wrinkle treatment. J. Kor. Soc. Cosm. 5, 615-631.
  14. Lee, H. K., Back, J. H., Koh, J. S., Park, D. H., Lee, J. S. and Jung, E. S. 2006. Correlation between skin roughness and dermal density of skin wrinkle evaluation. J. Soc. Cosmet. Scientists Korea 32, 123-127.
  15. Lee, S. H., Hong, S. J., Kim, S. Y., Yang, H. I., Lee, J. D., Choi, D. Y., Lee, D. I. and Lee, Y. H. 2003. Randomized controlled double blind study of bee venom therapy on rheu matoid arthritis. J. Acupunct. Res. 20, 9-17.
  16. Sangwan, N., Farooqi, A., Shabif, F. and Sangwan, R. 2001. Regulation of essential oil production in plants. Plant Growth Regul. 34, 3-21. https://doi.org/10.1023/A:1013386921596
  17. Yang, G., Lee, K., An, D. G., Lee, M. H., Ham, I. H. and Choi, H. Y. 2012. Effect of Chrysanthemi borealis flos on atopic dermatitis induced by 1-chloro 2, 4-dinitrobenzene in NC/ Nga mouse. Immunopharmacol. Immunotoxicol. 34, 413-418. https://doi.org/10.3109/08923973.2011.613401
  18. Yeo, J. J., Hsing, L. C., Yang, C. S., Jeong, S. I., Seo, E. S. and Jang, I. S. 2007. Effect of mahuang for weight loss in healthy adults : A double-blind, controlled, randomized, clinical Trial. J. Kor. Med. 28, 63-71.