Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characteristics of Phthalate Esters-exposed Boar Sperm during Boar Semen Storage

돼지 정액을 보관하는 동안 phthalate esters에 노출된 정자의 특성

  • Lee, A-Sung (College of Animal Life Science, Kangwon National University) ;
  • Lee, Sang-Hee (Institute of Animal Resources, Kangwon National University) ;
  • Lee, Seunghyung (College of Animal Life Science, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science, Kangwon National University)
  • 이아성 (강원대학교 동물생명과학대학) ;
  • 이상희 (동물자원공동연구소) ;
  • 이승형 (강원대학교 동물생명과학대학) ;
  • 양부근 (강원대학교 동물생명과학대학)
  • Received : 2018.11.19
  • Accepted : 2019.04.16
  • Published : 2019.04.30
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Abstract

Phthalate is a chemical endocrine disrupter and interfere with the action of hormones, estrogens, androgens and thyroid hormones. It also affect cardiovascular, metabolic, immune and reproductive system in the human and animals. Curcumin is antioxidant, anti-inflammatory activity and -cancer properties in the human. We studied whether phthalates damage viability, mitochondrial activity and membrane integrity of sperm in boar semen. We also treated curcumin with/without phthalates in the boar semen. Fresh boar semen was treated with phthalates and/or curcumin for examining sperm characteristics. Sperm characteristics, sperm motility, viability, mitochondrial activity, and membrane integrity were determined during storage of boar semen. Sperm motility and viability in dose-dependent manner decreased by di-n-butyl phthalate (DBP), mono-n-butyl phthalate (MBP) and di-2-ethylhexyl phthalate (DEHP, p<0.05). Phthalates also decreased mitochondrial activity and membrane integrity of sperm (p<0.05). However, sperm motility and viability were higher than untreated-curcumin when DBP, MBP and DEHP treated with a curcumin in boar semen (p<0.05). Mitochondrial activity and membrane integrity of sperm were higher in DBP- and MBP-treated semen with curcumin (p<0.05). In conclusion, phthalates can damage sperm viability and quality during the boar semen storage, and curcumin may protect the boar sperms from phthalates during storage term.

Phthalate는 내분비 교란물질로 호르몬의 변화, 에스토젠, 앤드로젠, 갑상선 호르몬의 분비를 방해한다. 또한, 인간과 동물에서 심혈관질환, 대사작용, 면역 및 번식체계에 영향을 끼친다. Curcumin은 항산화물질로 항염증 활성 및 항암작용에 영향을 미치는 것으로 알려져 있다. 본 연구는 phthalate가 정자의 운동성, 생존율, 미토콘드리아 활성 및 세포막 기능에 미치는 영향을 알아보고자 실시하였다. 또한, curcumin을 처리하여 phthalate에 노출된 정자에 미치는 영향을 분석하였다. 정자의 운동성과 생존율은 di-n-butyl phthalate (DBP), mono-n-butyl phthalate (MBP) 및 di-2-ethylhexyl phthalate (DEHP)을 처리하였을 때 감소하였다(p<0.05). Phthalate는 정자의 미토콘드리아 활성 및 세포막의 기능을 감소시켰다(p<0.05). 그러나, 정자의 운동성과 생존율은 curcumin을 처리하지 않은 것보다 처리한 정자에서 높게 나타났으며(p<0.05), 정자의 미토콘드리아 활성 및 세포막 기능에서도 높게 나타났다(p<0.05). 결론적으로, phthalate는 정자의 생존율과 세포의 기능에 영향을 미칠 수 있고, 이로부터 세포의 기능을 보호하기 위해서는 curcumin의 처리가 필요 할 것으로 생각된다.

Keywords

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Fig. 1. Effect of DBP, MBP, DEHP (50 μM) and/or curcumin (5 μM) on sperm motility in boar semen. Asterisks indicate significant differences compared with phthalate-treated sperm. Values represented as means ± SEM (p<0.05).

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Fig. 2. Effect of DBP, MBP, DEHP (50 μM) and/or curcumin (5 μM) on viability of sperm in boar semen. Asterisks indicate significant differences compared with phthalate-treated sample. Values represented as means ± SEM (p<0.05).

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Fig. 3. Effect of DBP, MBP, DEHP (50 μM) and/or curcumin (5 μM) on mitochondrial activity in boar semen. Asterisks indicate significant differences compared with phthalate-treated sample (p<0.05).

SMGHBM_2019_v29n4_395_f0004.png 이미지

Fig. 4. Effect of DBP, MBP, DEHP (50 μM) and/or curcumin (5 μM) on membrane integrity of sperm in boar semen. Asterisks indicate significant differences compared with phthalate-treated sperm. Values represented as means ± SEM (p<0.05).

Table 1. Composition of Modena extender

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Table 2. Effect of phthalates on sperm motility

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Table 3. Effect of phthalates on viability of sperm

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Table 4. Effect of phthalates on sperm mitochondrial activity

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Table 5. Effect of phthalates on membrane integrity of sperm

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