Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Dissipation of Bifenthrin and Chlorothalonil in Crown Daisy during Cultivation and their Biological Half-lives

쑥갓 중 bifenthrin과 chlorothalonil의 경시적 잔류량 변화 및 생물학적 반감기

  • Kang, Min-Seong (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Park, Po-Hyun (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Kim, Ki-Yu (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Lim, Bu-Geon (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Ryu, Kyong-Shin (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Lee, You-Jin (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Lim, Jeong-Hwa (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Kang, Choong-Won (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Kim, Youn-Ho (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Lee, Sun-Young (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Seo, Jeong-Hwa (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Park, Yong-Bae (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri) ;
  • Yoon, Mi-Hye (Guri Agro-fishery Products Inspection Center, Gyeonggi province Institute of Health and Environment Guri)
  • 강민성 (경기도보건환경연구원 구리농수산물검사소) ;
  • 박포현 (경기도보건환경연구원 구리농수산물검사소) ;
  • 김기유 (경기도보건환경연구원 구리농수산물검사소) ;
  • 임부건 (경기도보건환경연구원 구리농수산물검사소) ;
  • 유경신 (경기도보건환경연구원 구리농수산물검사소) ;
  • 이유진 (경기도보건환경연구원 구리농수산물검사소) ;
  • 임정화 (경기도보건환경연구원 구리농수산물검사소) ;
  • 강충원 (경기도보건환경연구원 구리농수산물검사소) ;
  • 김윤호 (경기도보건환경연구원 구리농수산물검사소) ;
  • 이선영 (경기도보건환경연구원 구리농수산물검사소) ;
  • 서정화 (경기도보건환경연구원 구리농수산물검사소) ;
  • 박용배 (경기도보건환경연구원 구리농수산물검사소) ;
  • 윤미혜 (경기도보건환경연구원 구리농수산물검사소)
  • Received : 2019.02.13
  • Accepted : 2019.04.02
  • Published : 2019.04.30
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Abstract

This study investigated the residual characteristics of bifenthrin and chlorothalonil in crown daisy and suggested pre-harvest residue limits (PHRLs) based on their dissipation patterns and biological half-lives. The samples for residue analysis were harvested at 0 (3 hr), 1, 3, 5, 7, 9, 11, 13, 15, 18, 22 and 26 days after treatment, and analyzed by $GC/{\mu}-ECD$ and TOF/MS. The limit of quantitation (LOQs) of bifenthrin and chlorothalonil were 0.0046 mg/kg and 0.0007 mg/kg, respectively. Recoveries ranged from $88.67{\pm}7.97%$ and $99.90{\pm}16.03%$, showing that this method is appropriate for the analysis of the pesticide residues in crown daisy. Being well within first order kinetics, the biological half-lives of the pesticide residues in crown daisy were 9.63 days for bifenthrin and 6.54 days for chlorothalonil. The PHRLs of bifenthrin and chlorothalonil were recommended as 11.70 mg/kg and 24.10 mg/kg for 26 days before harvest, respectively.

쑥갓의 안전성 확보를 위하여 쑥갓 재배기간 중 bifenthrin 및 chlorothalonil의 잔류양상을 연구하였다. 쑥갓에 유효성분 bifenthrin을 살포 후 0일(3시간)은 $0.90{\pm}0.07mg/kg$을 보였고, 26일후에는 $0.14{\pm}0.01mg/kg$이었으며, chlorothalonil은 0일(3시간)에 $79.12{\pm}6.02mg/kg$, 26일후에는 $4.60{\pm}1.33mg/kg$으로 나타났다. 쑥갓 중 bifenthrin과 chlorothalonil의 회수율은 각각 $88.67{\pm}7.97%$와 $99.90{\pm}16.03%$로 유효한 회수율 범위를 보였다. 쑥갓의 생육 기간 중 농약의 잔류량 변화는 first order kinetics model을 적용하여 해석한 결과 bifenthrin의 반감기는 9.63일이었으며, chlorothalonil은 6.54일이었다. 산출된 두 농약의 감소상수를 이용하여 생산단계 잔류 허용기준을 산출한 결과 bifenthrin의 수확 26일 전 잔류량은 11.7 mg/kg이었으며, chlorothalonil은 24.1 mg/kg이었는데 수확 예정일에는 두 농약의 잔류허용기준이 모두 5.0 mg/kg 이하로 잔류하여 안전할 것으로 판단되었다. 쑥갓 생산단계 농산물의 농약 잔류허용기준을 설정하는데 기초자료로 활용하여 농산물 유통단계의 부적합에 따른 생산자의 경제적 손실을 최소화하고 소비자에 대한 안전성을 강화할 수 있다고 사료된다.

Keywords

References

  1. Jung E.B. and Shin M.K.:In 'Hyang Yak Dae Jun', Young Lim Sa(3rd Ed.) Seoul, Korea, (1990).
  2. Kameoka, H. Kitagawa, C. Husebe, Y.:The constituents of the steam volatile oil from Chrysanthemum coronarium L. var spatiosum. J. Agri. Chem. Soc., 49, 652-657 (1975).
  3. Anyos, T. Steelink, C.:Fluresent petal constituents of Chrysanthemum coronarium L. Arch. Biochem. Biophys., 90, 63-67 (1960). https://doi.org/10.1016/0003-9861(60)90612-3
  4. Mahahiro, T. Kazuhiro, C.:Novel plant growth inhibitors and an insect antifeedant from Chrysanthemum coronarium L. Agric Biol. Chem., 48, 1367-1369 (1984). https://doi.org/10.1080/00021369.1984.10866324
  5. Fenik, J. Tankiewicz, M. Biziuk, M.:Properties and determination of pesticides in fruits and vegetables, Trac-Trend anal. Chem., 30, 814-826 (2011). https://doi.org/10.1016/j.trac.2011.02.008
  6. Jeong Y.H., Kim J.U., Lee Y.D., Lim C.H., Hur J.H.:Modern pesticide. first edition, Sigma-press, Seoul, Korea, pp. 5 (2004).
  7. Park J.H., Park J.S., Abd El-Aty., A.M. Rahman., M.M. Na., T.W. Shim J.H.:Analysis of imidacloprid and pyrimethanil in shallot(Allium ascalonicum) grown under greenhouse conditions using tandem mass spectrometry: establishment of pre-harvest residue limits, Biomed. Chromat., 27, 451-457 (2012). https://doi.org/10.1002/bmc.2812
  8. Lee J.H., Park Y.S., Kwon C.H., Lee Y.D., Kim, J. H.:Dissipation pattern of boscalid in cucumber under greenhouse condition. J. Pestic. Sci., 12, 67-73 (2008).
  9. Lee D.Y., Kim Y.J., Lee S.J., Cho K.S., Kim S.G., Park M.H., Kang K.Y.:Establishment of pre-harvest residue limit of fungicide pyrimethanil and trifloxystrobin during cultivation of persimmon. J. Environ. Agric., 31, 45-51 (2012). https://doi.org/10.5338/KJEA.2012.31.1.45
  10. Kim Y.S., Park J.H., Lee Y.D., Lee K,S., Kim J.E.:Persistence and dislodgeable residues of chlorpyrifos and procymidone in lettuce leaves under greenhouse condition. J. Environ. Agric., 21, 149-155 (2002). https://doi.org/10.5338/KJEA.2002.21.2.149
  11. Ko G.Y., Kim K.H., Lee K.S.:Residual pattern of procymidone and chlorthalonil in grape during the period of cultivation and storage. J. Environ. Agri., 23, 47-51 (2004).
  12. Ministry of Food and Drug Safety.:pre-harvest residue limits, (2018).
  13. Korea Crop Protection Association.:Guideline of Pesticide Use. Republic of Korea, 244-515 (2018).
  14. Ministry of Food and Drug Safety.:Analytical Practices Manual for Pesticide Residues in Foods, 5th Ed. 3, 80-81, (2017).
  15. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use.:Quality Guidelines, Validation of Analytical Procedures, Available from http://www.ich.org/fileadmin/PublicWebSite/ICH_Products/Guidelines/Quality/Q2R1Guideline.pdf.
  16. Lee D.Y., Kim Y.J., Park M.H., Lee S.H., Kim S.G., Kim N.J., Kang K.Y.:Establishment of pre-harvest residue limit (PHRL) of fungicides azoxystrobin and difenoconazole on prunus mume fruits. J. Pestic. Sci., 17, 307-313 (2013).
  17. Hwang E.J., Park J,E., Do J.A., Chung H.W., Chang H.R.:Residual Dissipation based on crop commodities classification of boscalid and spinetoram on crown daisy and sweet pepper under green houses., J. Environ. Agri., 36, 184-192 (2017).
  18. Bae B.J., Lee H.K., Son K.A., Im G.J., Kim J.B., Kim T.H., Park J.W.:The residue property of fungicide boscalid and fluidioxonil at the same time harvest leafy-vegetables. J. pestic. Sci., 16, 98-108 (2012).
  19. Lim J.S., Hong J.H., Lee C.R., Han K.T., Lee Y.R., Lee K.S.:Establishment of pre-harvest residue limit(PHRL) of insecticide chlorfenapyr and fungicide fenarimol during cultivation of chwinamul(Aster scaber). J. environ. Agric., 30, 52-59 (2011). https://doi.org/10.5338/KJEA.2011.30.1.52
  20. Ahn K.G., Kim G.H., Kim G.P., Kim M.J., Hong S.B., Hwang Y.S., Kwon C.H., Son Y.W., Lee Y.D., Choung M.G.:Establishment of pre-harvest residue limit(PHRL) of the fungicide amisulbrom during cultivation of winter-grown cabbage. J. Environ. Agric., 34, 120-127 (2015). https://doi.org/10.5338/KJEA.2015.34.2.15
  21. Marin, A. Oliva, J. Garcia, C. Navarro, S. Barba, A.:Dissipation rates of cyprodinil and fludioxonil in lettuce and table grape in the field and under cold storage conditions. J. Agric. Food Chem., 51, 4708-4711 (2003). https://doi.org/10.1021/jf021222e