Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제45권2호
  • /
  • Pages.97-107
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  • 2019
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
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Are critical size bone notch defects possible in the rabbit mandible?

  • Carlisle, Patricia L. (Department of Craniomaxillofacial Regenerative Medicine, Dental and Trauma Research Detachment) ;
  • Guda, Teja (Department of Biomedical Engineering, University of Texas at San Antonio) ;
  • Silliman, David T. (Department of Craniomaxillofacial Regenerative Medicine, Dental and Trauma Research Detachment) ;
  • Hale, Robert G. (Department of Craniomaxillofacial Regenerative Medicine, Dental and Trauma Research Detachment) ;
  • Baer, Pamela R. Brown (Department of Craniomaxillofacial Regenerative Medicine, Dental and Trauma Research Detachment)
  • 투고 : 2018.05.18
  • 심사 : 2018.08.03
  • 발행 : 2019.04.30
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초록

Objectives: Small animal maxillofacial models, such as non-segmental critical size defects (CSDs) in the rabbit mandible, need to be standardized for use as preclinical models of bone regeneration to mimic clinical conditions such as maxillofacial trauma. The objective of this study is the establishment of a mechanically competent CSD model in the rabbit mandible to allow standardized evaluation of bone regeneration therapies. Materials and Methods: Three sizes of bony defect were generated in the mandibular body of rabbit hemi-mandibles: $12mm{\times}5mm$, $12mm{\times}8mm$, and $15mm{\times}10mm$. The hemi-mandibles were tested to failure in 3-point flexure. The $12mm{\times}5mm$ defect was then chosen for the defect size created in the mandibles of 26 rabbits with or without cautery of the defect margins and bone regeneration was assessed after 6 and 12 weeks. Regenerated bone density and volume were evaluated using radiography, micro-computed tomography, and histology. Results: Flexural strength of the $12mm{\times}5mm$ defect was similar to its contralateral; whereas the $12mm{\times}8mm$ and $15mm{\times}10mm$ groups carried significantly less load than their respective contralaterals (P<0.05). This demonstrated that the $12mm{\times}5mm$ defect did not significantly compromise mandibular mechanical integrity. Significantly less (P<0.05) bone was regenerated at 6 weeks in cauterized defect margins compared to controls without cautery. After 12 weeks, the bone volume of the group with cautery increased to that of the control without cautery after 6 weeks. Conclusion: An empty defect size of $12mm{\times}5mm$ in the rabbit mandibular model maintains sufficient mechanical stability to not require additional stabilization. However, this defect size allows for bone regeneration across the defect. Cautery of the defect only delays regeneration by 6 weeks suggesting that the performance of bone graft materials in mandibular defects of this size should be considered with caution.

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참고문헌

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