Fig. 1. Agarose gel electrophoretic analysis of one-tube multiplex RT-PCR products amplified from total RNA preparations of virusinfected pear nursery tree tissues. RT-PCR products amplified from samples non-infected pear leaf (line 1); and water control (line 2); infected with different pome fruit viruses and viroid (lines 3-8). The bands corresponding to each virus have the following lengths: 202 bp for Apple scar skin viroid; 273 bp for Apple stem grooving virus; 367 bp for Apple stem pitting virus; 509 bp for Apple chlorotic leaf spot virus.
Table 1. Primer sequences and expected size for detection of pear viruses and viroid by multiplex RT-PCR
Table 2. The incidence of viral infection in pear nursery trees according to different locations and pear cultivars
Table 3. Single and mixed viral infection rates (%) in pear nursery trees according to different locations and pear cultivars
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