Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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The Incidence of Virus Diseases in Rehmannia glutinosa in Korea

국내 지황에 발생하는 바이러스병 발생 현황

  • Kwon, Sun-Jung (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoon, Ju-Yeon (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Choi, Gug-Seoun (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA)
  • 권선정 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 윤주연 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 조인숙 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 최국선 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Received : 2019.02.27
  • Accepted : 2019.03.16
  • Published : 2019.03.31
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Abstract

While rehmannia (Rehmannia glutinosa Libosch) was identified as a host of at least five viruses, including Rehmannia mosaic virus (ReMV), Youcai mosaic virus (YoMV), Broad bean wilt virus 2 (BBWV2), Plantago asiatica mosaic virus (PlAMV), and Rehmannia virus 1 (ReV1), viral incidence surveys have not been performed yet in rehmannia fields in Korea. In this study, we performed field surveys during 2017-2018 to investigate the incidence of 5 major viruses in rehmannia. A total of 145 symptomatic samples were collected from the rehmannia fields in major cultivation areas of Korea. Molecular diagnosis assays showed that all the collected leaf samples were infected with more than two viruses. Particularly, two species of Tobamovirus, ReMV and YoMV, were detected in all the samples. In addition, our analysis showed that the root stocks of 4 rehmannia cultivars were infected with at least two viruses. Since rehmannia is propagated by vegetative propagation, it is highly important to produce virus-free root stocks of rehmannia to control virus diseases in rehmannia.

최근 국내 지황에서는 Rehmannia mosaic virus (ReMV), Youcai mosaic virus (YoMV), Broad bean wilt virus 2 (BBWV2), Plantago asiatica mosaic virus (PlAMV), Rehamnnia virus 1 (ReV1) 등 5종의 바이러스 감염이 확인되었다. 국내 주요 지황 재배지역의 포장에서 지황 바이러스의 발병률을 조사하기 위해 2017년부터 2018년까지 여섯 지역의 포장에서 바이러스병 조사를 실시하였다. 지황에 나타나는 주요 바이러스 증상은 잎에 모자이크와 노란 반점 및 변색이었으며 생육이 저조해지는 증상이 관찰되었다. 총 145개의 시료를 채집하여 5종 바이러스에 대한 유전자 진단을 실시한 결과, 전 시료에서 2종 이상의 바이러스가 복합 감염되어 있음을 확인하였다. 지황은 뿌리로 영양번식하는 재배 특성 상 품종별 종근에서도 바이러스 감염여부를 조사하였으며, 종근에서도 5종의 바이러스가 검정됨에 따라 지황에서의 바이러스병 관리를 위해서는 무병 종근의 생산 및 보급이 매우 중요할 것으로 판단된다.

Keywords

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Fig. 1. Various virus symptoms on Rehmannia glutinosa (A) Yellow mosaic symptoms induced in plants co-infected with ReMV, YoMV, BBWV2, and ReV1. (B) Mosaic and leaf curl symptoms induced in plants co-infected with ReMV, YoMV, and BBWV2. (C) Vein necrosis and yellowing symptoms induced in plants co-infected with ReMV, YoMV, BBWV2, PlAMV, and ReV1.

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Fig. 2. Detection of virus particles from leaves (A) and roots (B) of Rehmannia glutinosa samples by transmission electron microscopy. Three types of virus particles (tobamovirus-like, potexvirus-like, and closterovirus-like particles as indicated with open arrows, triangle arrows and diamond arrows, respectively) were observed. Scale bar, 200 nm.

Table 1. List of primers used to detect viruses in rehmannia

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Table 2. Viral incidence in six rehmannia fields in Korea during 2017~2018

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Table 3. Types of Mixed infection of 5 viruses in rehmannia in Korea

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Table 4. Incidence of virus infection in tuberous root stocks of rehmannia

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