고랭이류에서 분리한 내생균의 생물다양성 및 지리적 양상

Geographical Patterns and Biodiversity of Endophytic Fungi Isolated from Scirpus L. s.l. in Korea

Abstract

고랭이류의 숙주식물인 Bolboschoenus planiculmis와 Schoenoplectustriqueter에서 내생균을 조사하였다. 총 25개체의 숙주식물에서 85개의 내생균을 분리하였고, ITS 지역을 사용하여 동정한 결과 19개의 분류군을 확인하였다. 그 중 92%는 Dothideomycetes에 속하였고, 4.8%는 Sordariomyetes, 1.6%는 Agaricomycetes, 0.8%는 Eurotiomycetes에 속하였다. B. planiculmis에서 가장 많이 분리된 내생균은 Cladosporium perangustum이고 S. triqueter에서는 Macrospora scirpicola였다. 염생식물에서 내생균의 연구는 매우 드물며, 이전에 목본식물들을 대상으로 수행된 선행연구들과는 다른 내생균의 군집 구조를 확인하였다. 향후 본 연구결과는 내생균 의 생물다양성 연구에 매우 유용할 것으로 생각된다.

We investigated the biodiversity of endophytic fungi of Bolboschoenus planiculmis and Schoenoplectus triqueter in Korea. Twenty five of these host plants were collected from six sampling sites. Internal transcribed spacer analysis identified a total of eighty five isolates from the collected plants. The isolates comprised nineteen taxa; most of the isolates (92%) belonged to class Dothideomycetes, followed by Sordariomyetes (4.8%), Agaricomycetes (1.6%), and Eurotiomycetes (0.8%). The most prevalent fungus was Cladosporium perangustum in B. planiculmis and Macrospora scirpicola in S. triqueter. There are very few studies of endophytic fungi in halophytes and these have reported biodiversity patterns different from those of previous studies in Korea. Our results should provide useful information for future research in Korea.

Fig. 1. Sampling sites of Bolboschoenus planiculmis (A: Saeseom, B: Youngsu, C: Donggeom, D:Myeongji) and Schoenoplectus triquerter (E: Eulsukdo, F: Songlim).

Fig. 2. Phylogenic tree of endophytic fungi from Bolboschoenus planiculmis and Schoenoplectus triquerter in seaboard of western and southern sea in Korea. ITS 1, ITS 2 and 5.8S rDNA region were used for the sequence analysis to confirm the topological appropriation of isolates. Sphatularia flavida was used as an out-group.

Fig. 3. Bray-Curtis ordination analysis to describe relationships of host plants, endophytic fungi and locality. Radiating lines indicate endophytic fungi species vector that affected locality heavily. Rectangles indicate indicate individual sampling sties of Bolboschoenus planiculmis and Circles indicate the onees of Schoenoplectus triquerter .

Table 1. Molecular identification of endophytic fungi isolated from Bolboschoenus planiculmis in Korea. A: Saeseom, B: Youngsu, C: Donggeom, D: Myeongji. Relative abundance indicates the percentages of the number of isolates in the study sites of the total numbers of isolates.

Table 2. Molecular identifcation of endophytic fungi isolated from Schoenoplectus triquerter in Korea.