한국균학회지 (The Korean Journal of Mycology)

  • 제47권1호
  • /
  • Pages.29-34
  • /
  • 2019
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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First Report of Allantophomopsiella pseudotsugae Isolated from Soil in Korea

  • Wajihi, Ally Hassan (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Das, Kallol (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education) ;
  • Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
  • 투고 : 2019.01.07
  • 심사 : 2019.03.15
  • 발행 : 2019.03.01
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초록

A fungal isolate designated 17E029 was isolated from a soil sample in Jeju, Korea. The strain was similar to other Allantophomopsiella species in its morphological characteristics such as grey mycelia, conidiophore, and conidia sizes. The isolate produced aerial mycelia, which appeared grey on the reverse side of the media surfaces and turned black on the front side of the colonies. The conidiophores emanating from the hyphae were hyaline, grey, aseptate, branched, and $6.7{\sim}9.2{\times}1.8{\sim}2.5{\mu}m$. Conidiogenous cells were ovoid to subcylindrical, discrete, guttulate, and hyaline. Conidia were hyaline, aseptate, smooth, guttulate, oval to subcylindrical, irregular in shape, and $6.0{\sim}7.8{\times}3.0{\sim}3.4{\mu}m$. The strain was confirmed based on phylogenetic analysis of the closest related organism, A. pseudotsugae CBS 288.37, using the partial 28S, internal transcribed spacer rDNA regions, and partial RNA polymerase II second largest subunit locus (RPB2) gene sequences along with its culture characteristics. Therefore, morphological observations and phylogenetic analysis revealed that strain 17E029 is similar to the previously identified A. pseudotsugae. Hence, this species was described as A. pseudotsugae strain 17E029, which is a new record in Korea.

키워드

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Fig. 1. Cultural characteristics of 17E029 (A~F), Colony on PDA (A, B), OA (C, D), and MEA (E, F)showing front and reverse sides.

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Fig. 2. Morphological characteristics of 17E029. A, Conidiophores; B, Conidiogenous cells; C, Conidia; Scale bars = 10 μm.

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Fig. 3. Neighbour-joining tree based on obtained sequences of the internal transcribed spacer rDNA region, LSU, and partial RNA polymerase II second largest subunit locus (RPB2). Sarcotrochila longispora strain CBS 273.74 was an outgroup. The fungal strain which examined in this study was indicated in bold and the bootstrap value below 70% is not shown. The scale bar, 0.01 indicates the number of nucleotide substitutions.

Table 1. GenBank codes of fungal strains used for phylogenetic analyses in this study.

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Table 2. Morphological characteristics of isolate 17E029 compared to the closest species Allantophomopsiella pseudotsugae

GNHHDL_2019_v47n1_29_t0002.png 이미지

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