Mycobiology

The Korean Society of Mycology (한국균학회)
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Genetic Diversity of the Pear Scab Fungus Venturia nashicola in Korea

  • Choi, Eu Ddeum (Pear Research Institute, National Institute of Horticultural & Herbal Science) ;
  • Kim, Gyoung Hee (Department of Plant Medicine, Sunchon National University) ;
  • Park, Sook-Young (Department of Plant Medicine, Sunchon National University) ;
  • Song, Jang Hoon (Pear Research Institute, National Institute of Horticultural & Herbal Science) ;
  • Lee, Young Sun (Department of Biology, Sunchon National University) ;
  • Jung, Jae Sung (Department of Biology, Sunchon National University) ;
  • Koh, Young Jin (Department of Plant Medicine, Sunchon National University)
  • Received : 2018.08.28
  • Accepted : 2018.12.24
  • Published : 2019.03.01
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Abstract

Scab disease caused by Venturia nashicola is of agroeconomic importance in cultivation of Asian pear. However, little is known about the degree of genetic diversity in the populations of this pathogen. In this study, we collected 55 isolates from pear scab lesions in 13 major cultivation areas in Korea and examined the diversity using sequences of internal transcribed spacer (ITS) region, ${\beta}$-tubulin (TUB2), and translation elongation factor-$1{\alpha}$ ($TEF-1{\alpha}$) genes as molecular markers. Despite a low level of overall sequence variation, we found three distinctive subgroups from phylogenetic analysis of combined ITS, TUB2, and $TEF-1{\alpha}$ sequences. Among the three subgroups, subgroup 1 (60% of isolates collected) was predominant compared to subgroup 2 (23.6%) or subgroup 3 (16.4%) and was distributed throughout Korea. To understand the genetic diversity among the subgroups, RAPD analysis was performed. The isolates yielded highly diverse amplicon patterns and none of the defined subgroups within the dendrogram were supported by bootstrap values greater than 30%. Moreover, there is no significant correlation between the geographical distribution and the subgroups defined by molecular phylogeny. Our data suggest a low level of genetic diversification among the populations of V. nashicola in Korea.

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References

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