미생물학회지 (Korean Journal of Microbiology)

  • 제55권1호
  • /
  • Pages.17-24
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  • 2019
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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Sphingomonas 속 세균의 명조건 생장에서 티아민의 필수적인 역할

Novel insight into the role of thiamine for the growth of a lichen-associated Arctic bacterium, Sphingomonas sp., in the light

  • 팜눙 (대구대학교 의생명과학과) ;
  • 팜코이 (대구대학교 의생명과학과) ;
  • 이창우 (대구대학교 의생명과학과) ;
  • 장세헌 (대구대학교 의생명과학과)
  • Pham, Nhung (Department of Biomedical Science and Center for Bio-Nanomaterials, Daegu University) ;
  • Pham, Khoi (Department of Biomedical Science and Center for Bio-Nanomaterials, Daegu University) ;
  • Lee, ChangWoo (Department of Biomedical Science and Center for Bio-Nanomaterials, Daegu University) ;
  • Jang, Sei-Heon (Department of Biomedical Science and Center for Bio-Nanomaterials, Daegu University)
  • 투고 : 2019.01.28
  • 심사 : 2019.03.14
  • 발행 : 2019.03.31
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초록

극지에 서식하는 세균은 강한 빛과 자외선을 받는다. 이 연구에서 우리는 북극에 서식하는 지의류 Cetraria sp.에서 분리한 호냉성 세균 Sphingomonas sp. PAMC 26621의 생장에 빛이 미치는 영향을 조사하였다. 이 세균은 암조건에서 명조건에서 보다 생장이 느렸다. 놀랍게도, 이 세균은 M9 최소배지에 티아민 혹은 아스코브르산을 첨가하면 명조건에서 생장이 증가하였지만, N-acetylcysteine을 첨가한 배지에서는 생장의 변화가 없었다. 첨가한 티아민과 아스코브르산은 포도당-6 인산 탈수소효소와 항산화 효소의 활성을 증가시켰다. 이 연구의 결과는 지의류와의 공생에서 제공된 티아민이 Sphingomonas sp. PAMC26621의 빛에 의한 산화적 스트레스를 완화시키는 항산화제 역할을 함을 의미한다. 이 연구는 강한 빛과 자외선이 만연한 북극에 서식하는 세균에 대한 생리적, 생화학적 관점에서 고찰할 점을 제시한다.

Bacteria in the polar region are under strong light and ultraviolet radiation. In this study, we investigated the effects of light on the growth of a psychrophilic bacterium, Sphingomonas sp. PAMC 26621, isolated from an Arctic lichen Cetraria sp. The growth of the strain in the light was lower than that in the dark. Surprisingly, thiamine increased the growth of Sphingomonas sp. PAMC 26621 in M9 minimal medium under light conditions. Thiamine increased the growth of the strain in a concentration-dependent manner along with ascorbic acid. N-acetylcysteine had no effect on the growth of the strain in the light. Thiamine and ascorbic acid also increased the activities of glucose-6-phosphate dehydrogenase and superoxide dismutase. The results of this study indicate that thiamine provided by the lichen symbiosis system plays an important role in light-induced oxidative stress in this Arctic bacterium as an antioxidant. Our study provide insight into the biochemistry and physiology of Arctic bacteria under strong light and ultraviolet radiation.

키워드

MSMHBQ_2019_v55n1_17_f0001.png 이미지

Fig. 1. The growth of Sphingomonas sp. PAMC 26621 in M9 medium and MT medium under light condition (A) and dark condition (B).

MSMHBQ_2019_v55n1_17_f0002.png 이미지

Fig. 2. The effects of antioxidants on the growth of Sphingomonas sp. PAMC 26621.

MSMHBQ_2019_v55n1_17_f0003.png 이미지

Fig. 3. Activities of G6PDH at mid-log and stationary phase on MT medium, M9 + thiamine, M9 + ascorbic acid, M9 (light), M9 (dark) (A).

MSMHBQ_2019_v55n1_17_f0004.png 이미지

Fig. 4. Native polyacrylamide gel stained for SOD activity of samples from Sphingomonas sp. PAMC 26621.

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