Physiological activity and efficacy of cosmetic products in bio-converted soybean embryo extract

생물전환 콩 배아추출물의 생리활성 및 화장품의 효능 연구

  • Min, Kyung-Nam (Department of Cosmetics inventive Design, Anyang University) ;
  • Lee, Gun-Hee (Department of Microbio Engineering, Konkuk University) ;
  • Park, Sung-Jin (Department of Food and Biotechnology, Korea University) ;
  • Choe, Tae-Boo (Department of Cosmetics Engineering, Konkuk University)
  • 민경남 (안양대학교 화장품발명디자인학과) ;
  • 이건희 (건국대학교 생물공학과) ;
  • 박성진 (고려대학교 식품생명공학과) ;
  • 최태부 (건국대학교 화장품공학과)
  • Received : 2018.09.18
  • Accepted : 2019.03.20
  • Published : 2019.03.28


This study was to investigate the physiological activities of bioconversion soybean embryo extract and the effects of sleeping pack containing extract on skin. The aglycone isoflavones of the soybean embryo extract bioconversed with pectinase were identified by HPLC. in vitro tests showed that they were non-toxic, anti-inflammatory, anti-melanin and anti-aging activity. After 4 weeks of human body application with the sleeping pack using extract, the skin surface roughness and eye wrinkles were so improved by low stimulation that the sleeping pack containing bioconversion soybean embryo extract has a positive effect on the skin. We confirmed that the bioconversion soybean embryo extract was effective in enhancing the physiological activity and was useful as a cosmetic material of various formulations.

생물전환한 콩 배아추출물을 미용학적 측면에서의 생리활성을 연구하고 이를 함유한 수면 팩이 피부에 미치는 영향을 규명하고자 하였다. 이를 위해 Pectinase로 생물전환 한 콩 배아추출물을 HPLC로 비배당체 이소플라본을 확인하였고, in vitro 실험한 결과 무독성, 항염증, 항멜라닌, 항노화의 활성이 나타났다. 이후 이를 함유한 수면 팩을 제조하여 4주간 인체적용시험한 결과 저자극으로 피부의 거칠기, 눈가 주름을 유의미하게 개선하여 생물전환 콩 배아추출물을 함유한 수면팩은 피부에 긍정적인 효과가 있는 것으로 나타났다. 이 연구를 통해 생물전환 콩 배아추출물은 피부의 생리 활성을 증진시키는 효능이 있고 다양한 제형의 화장품 소재로 활용 가치가 있는 것으로 확인되었다.


OHHGBW_2019_v10n3_211_f0001.png 이미지

Fig. 1. HPLC chromatogram of extract of soybean germ. (A) Daidzin (B) Genistin (C) Daidzein (D) Genistein

OHHGBW_2019_v10n3_211_f0002.png 이미지

Fig. 2. Amount of isoflavone isolated from Soybean germ.

OHHGBW_2019_v10n3_211_f0003.png 이미지

Fig. 3. Bioconversioin yield of isoflavones extracted from soybean germ using Pectinase.

OHHGBW_2019_v10n3_211_f0004.png 이미지

Fig. 4. Effect of bioconversioned soybean germ extract on cell viability. Result is resented as mean ± SD. (A) RAW 264.7 cells (B) B16F10 cells (C) HDF cells.

OHHGBW_2019_v10n3_211_f0005.png 이미지

Fig. 5. Inhibitory effect of bioconversioned soybean germ extract on LPS-induced NO production by RAW 264.7 cells. Result is represented as mean ± SD. *p<0.05

OHHGBW_2019_v10n3_211_f0006.png 이미지

Fig. 6. Inhibition of melanin synthesis in B16F10 melanoma cells treated with bioconversioned soybean germ extract. Result is represented as mean ± SD. *p<0.05 **p<0.01 ***p<0.001

OHHGBW_2019_v10n3_211_f0007.png 이미지

Fig. 7. Inhibitory effects of bioconversioned soybean germ extract on UVB-induced MMP-1 in HDF cells. Result is represented as mean ± SD. **p<0.01 ***p<0.001

Table 1. Gradient condition for HPLC analysis

OHHGBW_2019_v10n3_211_t0001.png 이미지

Table 2. Formulation of wash-off pack containing bioconversioned soybean germ extract

OHHGBW_2019_v10n3_211_t0002.png 이미지

Table 3. Irritant classification criteria and skin irritation score system (A) Irritant classification criteria (B) skin rritation score systemA

OHHGBW_2019_v10n3_211_t0003.png 이미지

Table 4. Changes in roughness of skin.

OHHGBW_2019_v10n3_211_t0004.png 이미지

Table 5. Changes in roughness of skin.

OHHGBW_2019_v10n3_211_t0005.png 이미지


  1. J. H. Son, M. G. Choung, H. J. Choi, U. B. Jang, G. M. Son, M. W. Byun, C. Choi. (2001). Physiological Effect of Korean Black Soybean Pigment. Korean Journal of Food Science and Technology. 33(6), 764-768.
  2. C. H. Kim, J. S. Park, H. S. Sphn, C. W. Chung. (2002). Determination of Isoflavone, Total Saponin, Dietary Fiber, Soy Oligosaccharides and Lecithins from Commercial Soy Products Based on the One Serving Size - Some bioactive compounds from commercialized soy products. Korean Journal of Food Science and Technology. 34(1), 96-102.
  3. J. H. Shin, N. M. Joo. (2016). Component Changes in Antioxidant Activity and Isoflavones (${\beta}$-glucoside & aglycone) Contents of Small Black Bean According to Different Cooking Methods. Korean Journal of Food & Cookery Science, 32(2), 10. DOI : 9724/kfcs.2016.32.2.197
  4. H. Y. Park, E. J. Cho. (2008). Radical Scavenging Effects and Physicochemical Properties of Seolitae Chungkukjang Added with Green Tea. Journal of the Korean Society of Food Science and Nutrition, 37(4), 401-404. DOI : 10.3746/jkfn.2008.37.4.401
  5. H. Y. Cho, J. L. Yang, K. H. Noh, J. J. Kim, Y. H. Kim, K. H. Huh, Y. S. Song. (2007). Anti-atherogenic Effect of Isoflavone through Hypolipidemic, Anti-oxidative and Anti-inflammatory Actions in C57BL/6 Mice. Korean society of food and nutrition, 36(3), 276-283. DOI : 10.3746/jkfn.2007.36.3.276
  6. H. G. Kim, M. J. Lee, H. J. Kim, K. C. Kim, Shambhunath Bose. (2012). Effects of Fermented Soybean upon Anti.inflammation and Intestinal Mucous Membrane Permeability. Journal of Society of Korean Medicine for Obesity Research, 12(1), 33-47.
  7. Xin Zhao, J. H. Ju, K. Y. Park. (2017). Comparisons of Properties, In Vitro Anti-Mutagenicity, and Anti-Cancer Effects of Short-Term Fermented Soybean Foods (Chungkukjang, Shuidouchi, and Natto). The Korean Society of Food Science and Nutrition, 46(10), 1253-1257.
  8. S. Y. Lim, K. Y. Park, M. S. Bae, K. H. Kim. (2009). Effect of Doenjang with Black Soybean on Cytokine Production and Inhibition of Tumor Metastasis. Journal of Life Science, 19(2), 264-270. DOI :10.5352/jls.2009.19.2.264
  9. J. H. Lee, Y. S. Park, H. M. Park, J. M. Heo. (2012). Survey on the Consumption of the Phytoestrogen Isoflavone in Postmenopausal Korean Women. Journal of Menopausal Medicine, 18(3), 163-173. DOI : 10.6118/jksm.2012.18.3.163
  10. K. W. Kim, B. S. Chun. (2001). Optimum Conversion to the Aglycone Form Using ${\beta}$-glucosidase and Isoflavone Extraction from Soybean. KSBB Journal, 16(2), 174-178.
  11. D. S. Lee, S. Y. Byun. (2001). Effects of the Dietary Mixture of Eucommia ulmoides Oliver on Osteoporosis. KSBB Journal, 16(6), 614-619.
  12. B. G. Kim, C. K. Oh, J. H. Jo, J. B. Lee, M. B. Kim, H. S. Jang, K. S. Kwon. (2003). Effects of Estrogens and Isoflavones on the Collagen Synthesis. Korean Journal of Dermatology. 41(8), 1041-1048.
  13. M. S. Lee, S. H. Li. (2010). Research on the perception of fermented cosmetics and the satisfaction level of 'Korean Medicine Herb' cosmetics. Korean journal of aesthetics and cosmetics society, 8(3), 285-292.
  14. M. S. Joo, S. H. Li. (2012). The Effects on Middle-aged Women's Skin with Resveratrol Cosmetics and Supplement Intake. Korean journal of aesthetics and cosmetics society, 18(3), 643-650.
  15. K. H. Park, X. M. Piao, E. K. Jang, Y. E. Yoo, T. Y. Hwang, S. L. Kim, J. H. Jong, H. M. Shin. (2012). Variation of Isoflavone Contents in Korean Soybean Cultivars Released from 1913 to 2006. Korean Journal of Breeding Science, 44(2), 149-159
  16. A. NAKAMURA, H. FURUTA, H. MAEDA, Y. NAGAMATSU, A. YOSHIMOTO. (2001). Analysis of Structural Components and Molecular Construction of Soybean Soluble Polysaccharides by Stepwise Enzymatic Degradation. Biotechnology and Biochemistry, 65(10), 2249-2258. DOI : 10.1271/bbb.65.2249
  17. G. Repetto, Ana del Peso, J. L Zurita. (2008). Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature Protocols, 3, 1125-1131. DOI : 10.1038/nprot.2008.75
  18. David S Chi, Min Qui, Guha Krishnaswamy, Chuanfu Li, William Stone. (2003). Regulation of nitric oxide production from macrophages by lipopolysaccharide and catecholamines. Nitric Oxide. 8(2). 127-132. DOI : 10.1016/s1089-8603(02)00148-9
  19. D. S. Kim, Y. M. JEONG, I. K. PARK, H. G. HAHN, H. K. LEE, S. B. KWON, J. H. JEONG, S. J. YANG, U. D. SOHN, K. C. PARK. (2007). A New 2-Imino-1,3-thiazoline Derivative, KHG22394, Inhibits Melanin Synthesis in Mouse B16 Melanoma Cells. Biol. Pharm. Bull. 30(1), 180-183. DOI :10.1248/bpb.30.180
  20. R. Korhonen, A. Lahti, H. Kankaanranta, E. Moilanen. (2005). Nitric oxide production and signaling in inflammation. Current Drug Targets, 4(4), 471-479. DOI : 10.2174/1568010054526359
  21. Hamalainen M1, Nieminen R, Vuorela P, Heinonen M, Moilanen E. (2007). Anti-Inflammatory Effects of Flavonoids: Genistein, Kaempferol, Quercetin, and Daidzein Inhibit STAT-1 and $NF-{\kappa}B$ Activations, Whereas Flavone, Isorhamnetin, Naringenin, and Pelargonidin Inhibit only $NF-{\kappa}B$ Activation along with Their Inhibitory Effect on iNOS Expression and NO Production in Activated Macrophages. Mediators of Inflammation. DOI : 10.1155/2007/45673
  22. DENISE J. SCHWAHN, WEIDONG XU, AMEY B. HERRIN, ELISE S. BALES, ESTELA E. MEDRANO. (2002). Tyrosine Levels Regulate the Melanogenic Response to alpha-Melanocyte-Stimulating Hormone in Human Melanocytes: Implications for Pigmentation and Proliferation. Pigment Cell Research.. 14(1), 32-39. DOI : 10.1034/j.1600-0749.2001.140106
  23. SewonKangJin, HoChung1Joo, HeungLee2Gary, J.FisherYian, ShengWanElizabeth, A.DuellJohn J.Voorhees. (2016). Molecular mechanisms of flavonoids in melanin synthesis and the potential for the prevention and treatment of melanoma. Nutrition and Cancer. 60(6), 1264-1274. DOI : 10.1002/mnfr.201500822
  24. Kelly K. Dong, Niusha Damaghi, Stephanie D. Picart, Nelli G. Markova, Kei Obayashi, Yuri Okano, Hitoshi Masaki, Susanne Grether-Beck, Jean Krutmann, Kenneth A. Smiles, Daniel B. Yarosh. (2008). UVinduced DNA damage initiates release of MMP-1 in human skin. Experimental Dermatology, 17(12), 1037-1044. DOI : 10.1111/j.1600-0625.2008.00747
  25. Sewon Kang Jin, Ho Chung Joo, Heung Lee, Gary. J. Fisher Yian, Sheng Wan Elizabeth, A. DuellJohn, J. Voorhees. (2002). Topical N-Acetyl Cysteine and Genistein Prevent Ultraviolet-Light-Induced Signaling That Leads to Photoaging in Human Skin in vivo. Nutrition and Cancer. 60(6), 1264-1274. DOI : 10.1046/j.1523-1747.2003.12122.x
  26. B. Y. Sin, H. P. Kim. (2005). Inhibition of collagenase by naturally-occurring flavonoids. Archives of Pharmacal Research. 28(10), 1152- 1155. DOI : 10.1007/BF02972978