Fig. 2. Effect of methanol extract from Psidium guajava L. leaf on the intracellular reactive oxygen species (ROS) formation induced by tert-butyl hydroperoxide (TBHP).
Fig. 3. Effect of methanol extract from Psidium guajava L. leaf on tert-butyl hydroperoxide (TBHP)-induced cellular redox state impairment.
Fig. 4. Antioxidant enzyme activity of glutathione reductase (A) and glutathione peroxidase (B) were evaluated in HepG2 cells treated with 1 mM tert-butyl hydroperoxide (TBHP).
Fig. 1. (A) Cytotoxicity and (B) cell protective effect of methanol extract from Psidium guajava L. leaf methanol extracts in HepG2 cells.
Table 1. Total polyphenols and flavonoid contents of the methanolic extract of Psidium guajava L. leaf.
Table 2. ABTS and DPPH free radical scavenging activity of the methanolic extract of Psidium guajava L. leaf.
참고문헌
- Aruoma OI. 1998. Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc 75:199-212 https://doi.org/10.1007/s11746-998-0032-9
- Baker MA, Cerniglia GJ, Zaman A. 1990. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal Biochem 190: 360-365 https://doi.org/10.1016/0003-2697(90)90208-Q
- Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200 https://doi.org/10.1038/1811199a0
- Cederbaum AI, Lu Y, Wu D. 2009. Role of oxidative stress in alcohol-induced liver injury. Arch Toxicol 83:519-548 https://doi.org/10.1007/s00204-009-0432-0
- Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12:239-243 https://doi.org/10.1016/S0021-9258(18)88697-5
- Halliwell B, Gutteridge JMC. 1999. Free Radicals in Biology and Medicine. 3rd ed. pp.105-350. Oxford University Press
- Hsieh CL, Huang CN, Lin YC, Peng RY. 2007. Molecular action mechanism against apoptosis by aqueous extract from guava budding leaves elucidated with human umbilical vein endothelial cell (HUVEC) model. J Agric Food Chem 55: 8523-8533 https://doi.org/10.1021/jf071858b
- Jeong EJ, Kim KP, Bang BH. 2012. Quality characteristics of cookies added with guava (Psidium guajava L.) leaf powder. Korean J Food Nutr 25:317-323 https://doi.org/10.9799/ksfan.2012.25.2.317
- Jiao HL, Ye P, Zhao BL. 2003. Protective effects of green tea polyphenols on human HepG2 cells against oxidative damage of fenofibrate. Free Radical Biol Med 35:1121-1128 https://doi.org/10.1016/S0891-5849(03)00506-9
- Jin YJ, Kang SH, Choi SY, Park SY, Park JG, Moon SW, Park DB, Kim SJ. 2006. Effect of fermented guava (Psidium guajava L.) leaf extract on hyperglycemia in low dose streptozotocin-induced diabetic mice. Korean J Food Sci Technol 38:679-683
- Jo YH, Ok DL, Lee SC. 2009. Antimicrobial characteristics of different parts of guava against food-borne bacteria. J Korean Soc Food Sci Nutr 38:1773-1778 https://doi.org/10.3746/jkfn.2009.38.12.1773
- Lee KM, Kwon TY, Kang U, Seo EK, Yun JH, Nho CW, Kim YS. 2017. Tussilagonone-induced Nrf2 pathway activation protects HepG2 cells from oxidative injury. Food Chem Toxicol 108:120-127 https://doi.org/10.1016/j.fct.2017.07.035
- Lozoya X, Reyes-Morales H, Chavez-Soto MA, Martinez-Garcia MDC, Soto-Gonzalez Y, Doubova SV. 2002. Intestinal antispasmodic effect of a phytodrug of Psidium guajava folia in the treatment of acute diarrheic disease. J Ethnopharmacol 83:19-24 https://doi.org/10.1016/S0378-8741(02)00185-X
- Maisuthisakul P, Suttajit M, Pongsawatmanit R. 2007. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem 100:1409-1418 https://doi.org/10.1016/j.foodchem.2005.11.032
- Ministry of Food and Drug Safety. 2016. Health Functional Food Code. p.2,11,96. Ministry of Food and Drug Safety
- Mosbah H, Louati H, Boujbiha MA, Chahdoura H, Snoussi M, Flamini G, Ascrizzi R, Bouslema A, Achour L, Selmi B. 2018. Phytochemical characterization, antioxidant, antimicrobial and pharmacological activities of Feijoa sellowiana leaves growing in Tunisia. Ind Crops Prod 112:521-531 https://doi.org/10.1016/j.indcrop.2017.12.051
- Moskaug JO, Carlsen H, Myhrstad MCW, Blomhoff R. 2005. Polyphenols and glutathione synthesis regulation. Am J Clin Nutr 81:277-283 https://doi.org/10.1093/ajcn/81.1.277S
- Nantitanon W, Yotsawimonwat S, Okonogi S. 2010. Factors influencing antioxidant activities and total phenolic content of guava leaf extract. LWT-Food Sci Technol 43:1095-1103 https://doi.org/10.1016/j.lwt.2010.02.015
- Nishida K, Ohta Y, Ishiguro I. 1997. Modulating role of endogenous reduced glutathione in tert-butyl hydroperoxide- induced cell injury in isolated rat hepatocytes. Free Radical Biol Med 23:453-462 https://doi.org/10.1016/S0891-5849(97)00116-0
- Pandey KB, Rizvi SI. 2009. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev 2:270-278 https://doi.org/10.4161/oxim.2.5.9498
- Park BJ, Onjo M. 2008. Antioxidant activities and tyrosinase inhibitory effects of guava (Psidium guajava L.) leaf. Korean J Plant Resour 21:408-412
- Paul LW. 1999. Biochemical diagnosis of liver disease. Indian J. Clin Biochem 14:59-90 https://doi.org/10.1007/BF02869152
- Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
- Rees MD, Kennett EC, Whitelock JM, Davies MJ. 2008. Oxidative damage to extracellular matrix and its role in human pathologies. Free Radical Biol Med 44:1973-2001 https://doi.org/10.1016/j.freeradbiomed.2008.03.016
- Sies H. 1999. Glutathione and its role in cellular functions. Free Radical Biol Med 27:916-921 https://doi.org/10.1016/S0891-5849(99)00177-X
- Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S. 2007. Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chem 103:381-388 https://doi.org/10.1016/j.foodchem.2006.07.034
- Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44-84 https://doi.org/10.1016/j.biocel.2006.07.001
- Yeon SH, Ham H, Sung J, Kim Y, Namkoong S. Jeong HS, Lee J. 2013. Antioxidant activities of hot water extract from Cornus walteri Wanger against oxidative stress induced by tert-butyl hydroperoxide in HepG2 cells. J Korean Soc Food Sci Nutr 42:1525-1532 https://doi.org/10.3746/jkfn.2013.42.10.1525
- Yoon TJ, Jo SY. 2010. Effect of acanthopanax senticosus extracts on alcohol degradation and anti-inflammatory activity in mice. Korean J Food Nutr 4:542-548
- Zhang XH, Choi SK, Seo JS. 2009. Effect of dietary grape pomace on lipid oxidation and related enzyme activities in rats fed high fat diet. Korean J Nutr 42:415-422 https://doi.org/10.4163/kjn.2009.42.5.415
- Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
피인용 문헌
- 산화적 스트레스에 대한 여주(Momordica charantia Linn.)잎의 항산화 활성 및 간세포 보호능 vol.35, pp.6, 2019, https://doi.org/10.7318/kjfc/2020.35.6.597