Journal of Microbiology and Biotechnology

  • 제29권2호
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  • Pages.268-273
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  • 2019
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Altering UDP-Glucose Donor Substrate Specificity of Bacillus licheniformis Glycosyltransferase towards TDP-Glucose

  • Cho, Kye Woon (Department of Life Science and Biochemical Engineering, Sun Moon University) ;
  • Kim, Tae-Su (Department of Life Science and Biochemical Engineering, Sun Moon University) ;
  • Le, Tuoi Thi (Department of Life Science and Biochemical Engineering, Sun Moon University) ;
  • Nguyen, Hue Thi (Department of Life Science and Biochemical Engineering, Sun Moon University) ;
  • Oh, So Yeong (Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University Chungnam) ;
  • Pandey, Ramesh Prasad (Department of Life Science and Biochemical Engineering, Sun Moon University) ;
  • Sohng, Jae Kyung (Department of Life Science and Biochemical Engineering, Sun Moon University)
  • 투고 : 2018.11.08
  • 심사 : 2018.12.10
  • 발행 : 2019.02.28
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초록

The specificity of a Bacillus licheniformis uridine diphosphate (UDP) glycosyltransferase, YjiC, was increased towards thymidine diphosphate (TDP)-sugar by site-directed mutagenesis. The Arg-282 of YjiC was identified and investigated by substituting with Trp. Conversion rate and kinetic parameters were compared between YjiC and its variants with several acceptor substrates such as 7-hydroxyflavone (7-HF), 4',7-dihydroxyisoflavone, 7,8-dihydroxyflavone and curcumin. Molecular docking of TDP-glucose and 7-HF with YjiC model showed pi-alkyl interaction with Arg-282 and His-14, and pi-pi interaction with $His^{14}$ and thymine ring. YjiC (H14A) variant lost its glucosylation activity with TDP-glucose validating significance of His-14 in binding of TDP-sugars.

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참고문헌

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