Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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A Study of the Diversity and Profile for Extracellular Enzyme Production of Aerobically Cultured Bacteria in the Gut of Muraenesox cinereus

갯장어(Muraenesox cinereus) 장으로부터 호기적 조건에서 분리된 미생물의 다양성 및 세포외 효소 생산능 분석에 관한 연구

  • Lee, Yong-Jik (Department of Cosmetics, Seowon University) ;
  • Oh, Do-Kyoung (Major in Food Biotechnology and Research Center for Extremophiles & Marine Microbiology, Silla University) ;
  • Kim, Hye Won (Major in Food Biotechnology and Research Center for Extremophiles & Marine Microbiology, Silla University) ;
  • Nam, Gae-Won (Department of Cosmetics, Seowon University) ;
  • Sohn, Jae Hak (Major in Food Biotechnology and Research Center for Extremophiles & Marine Microbiology, Silla University) ;
  • Lee, Han-Seung (Major in Food Biotechnology and Research Center for Extremophiles & Marine Microbiology, Silla University) ;
  • Shin, Kee-Sun (Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Sang-Jae (Major in Food Biotechnology and Research Center for Extremophiles & Marine Microbiology, Silla University)
  • 이용직 (서원대학교 화장품학부) ;
  • 오도경 (신라대학교 식품공학전공 & 해양극한미생물연구소) ;
  • 김혜원 (신라대학교 식품공학전공 & 해양극한미생물연구소) ;
  • 남개원 (서원대학교 화장품학부) ;
  • 손재학 (신라대학교 식품공학전공 & 해양극한미생물연구소) ;
  • 이한승 (신라대학교 식품공학전공 & 해양극한미생물연구소) ;
  • 신기선 (한국생명공학연구원 산업바이오 소재연구센터) ;
  • 이상재 (신라대학교 식품공학전공 & 해양극한미생물연구소)
  • Received : 2019.01.26
  • Accepted : 2019.02.27
  • Published : 2019.02.28
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Abstract

This research confirmed the diversity and characterization of gut microorganisms isolated from the intestinal organs of Muraenesox cinereus, collected on the Samcheonpo Coast and Seocheon Coast in South Korea. To isolate strains, Marine agar medium was basically used and cultivated at $37^{\circ}C$ and pH7 for several days aerobically. After single colony isolation, totally 49 pure single-colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rRNA gene DNA sequencing, indicating that isolated strains were divided into 3 phyla, 13 families, 15 genera, 34 species and 49 strains. Proteobacteria phylum, the main phyletic group, comprised 83.7% with 8 families, 8 genera and 26 species of Aeromonadaceae, Pseudoalteromonadaceae, Shewanellaceae, Enterobacteriaceae, Morganellaceae, Moraxellaceae, Pseudomonadaceae, and Vibrionaceae. To confirm whether isolated strain can produce industrially useful enzyme or not, amylase, lipase, and protease enzyme assays were performed individually, showing that 39 strains possessed at least one enzyme activity. Especially the Aeromonas sp. strains showed all enzyme activity tested. This result indicated that isolated strains have shown the possibility of the industrial application. Therefore, this study has contributed for securing domestic genetic resources and the expansion of scientific knowledge of the gut microbial community in Muraenesox cinereus of South Korea.

국내 삼천포 및 서천에서 채집한 갯장어(Muraenesox cinereus)의 장내기관으로부터 분리된 미생물들의 다양성 및 특성에 관하여 조사하였다. 장내 미생물의 순수 분리를 위하여 marine agar 배지를 사용하였으며 $37^{\circ}C$에서 호기적으로 배양하였다. 순수 분리 후, 49 균주를 분리하였으며 16S rRNA 염기서열 분석 결과를 바탕으로 계통학적 분석을 실시한 결과, 3문, 13과, 14속, 34종으로 구성되어 있는 것을 확인하였다. 특히, Proteobacteria 문은 83.7%의 분포를 나타내었으며 8과, 8속, 26종으로 Aeromonadaceae, Pseudoalteromonadaceae, Shewanellaceae, Enterobacteriaceae, Morganellaceae, Moraxellaceae, Pseudomonadaceae와 Vibrionaceae로 분포하는 것을 확인하였다. 그리고 분리한 균주들이 amylase, lipase, protease와 같은 산업적으로 유용한 효소를 생산하는지 확인하기 위하여 효소 활성 평가를 실시하였으며, 39 균주가 최소 한 종류 이상의 효소 활성을 가지고 있는 것을 확인하였다. 특히 Aeromonas 속의 균주들은 테스트한 모든 효소의 활성을 나타내는 것을 확인하였다. 이러한 결과는 본 연구를 통하여 분리한 미생물들의 산업적 활용 가능성을 나타내었다. 그러므로 이번 연구는 국내 유전자원 확보 및 갯장어의 장내마이크로비옴의 과학적 지식 확장에 도움이 될 것으로 생각된다.

Keywords

SMGHBM_2019_v29n2_248_f0001.png 이미지

Fig. 1. Neighbour-joining phylogenetic tree determined from the 16S rDNA sequences of bacteria from the gut of Muraenesox cinereus. Bootstrap values calculated (from 1,000 replications).

Table 1. Isolation and identification of aerobically cultured microorganisms isolated from the gut microbiomes of Muraenesox cinereus

SMGHBM_2019_v29n2_248_t0001.png 이미지

Table 2. Isolation and identification of aerobically cultured microorganisms isolated from the gut microbiomes of Muraenesox cinereus

SMGHBM_2019_v29n2_248_t0002.png 이미지

Table 3. Representative sequences of an aerobically isolated strains from the gut microbiomes of Muraenesox cinereus

SMGHBM_2019_v29n2_248_t0003.png 이미지

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